Mutational Analysis of DNASE I Gene in Diabetic Patients

Authors

  • Nageen Hussain Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan.
  • Hateem Zafar Kayani Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan.

DOI:

https://doi.org/10.24297/jbt.v3i3.5025

Keywords:

Deoxyribonuclease I, Polymerase Chain Reaction, Insulin-Dependent Diabetes Mellitus, Non-Insulin Dependent Diabetes Mellitus.

Abstract

The main aim is to analyze the mutations of DNASE I gene in diabetic patients.A total of 120 diabetes patients and 120 controls were sampled. The total number of male diabetic patients included in the study was 79 (66%) while female patients were 41 (34%) in number. Exon 8 of the DNASE I gene was amplified by using thermocycler. The possible band of interest was located at 165 base pairs. Two samples showed similar missense mutations at 127th position of exon 8 which replaced amino acid Arginine (Arg) to Glutamine (Gln). All controls showed no mutations. The association of diabetes with different levels of blood pressure and body mass index (BMI) were found to be significant.

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Author Biographies

Nageen Hussain, Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan.

Department of Microbiology and Molecular Genetics.

Hateem Zafar Kayani, Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan.

Department of Microbiology and Molecular Genetics.

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Published

2014-07-18

How to Cite

Hussain, N., & Kayani, H. Z. (2014). Mutational Analysis of DNASE I Gene in Diabetic Patients. JOURNAL OF ADVANCES IN BIOTECHNOLOGY, 3(3), 242–247. https://doi.org/10.24297/jbt.v3i3.5025

Issue

Vol. 3 No. 3

Section

Articles

License

Creative Commons License All articles published in Journal of Advances in Linguistics are licensed under a Creative Commons Attribution 4.0 International License.