Association Between ACE I/D Gene Polymorphism and Hypertension in Smokers

Authors

  • Hateem Zafar Kayani Department of Microbiology and Molecular Genetics, Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan.
  • Sana Riaz Department of Microbiology and Molecular Genetics, Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan.
  • Nageen Hussain Department of Microbiology and Molecular Genetics, Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan.

DOI:

https://doi.org/10.24297/jbt.v4i1.5006

Keywords:

Angiotensin converting enzyme, Polymerase chain reaction, Genotype, Polymorphism.

Abstract

The main aim is to study the association of ACE I/D polymorphism with healthy smokers. A total of 49 smokers and 49 healthy controls were enrolled in this study. Intron 16 of the ACE gene was amplified by using nested PCR. The possible banding patterns were as follows; DD, 210 bp fragments; II, 264bp and 498 bp fragments; ID, 210 bp, 264 bp, and 498 bp fragments. The subjects comprised of males only. Among 49 subjects the frequency of ACE DD genotype was 75.5% (37/49), II 24.5% (12/49), while no ID genotype was found in the study. All controls showed DD genotype. The association of ACE I/D polymorphism with different levels of blood pressure were found to be significant.

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Published

2014-09-01

How to Cite

Zafar Kayani, H., Riaz, S., & Hussain, N. (2014). Association Between ACE I/D Gene Polymorphism and Hypertension in Smokers. JOURNAL OF ADVANCES IN BIOTECHNOLOGY, 4(1), 336–342. https://doi.org/10.24297/jbt.v4i1.5006

Issue

Vol. 4 No. 1

Section

Articles

License

Creative Commons License All articles published in Journal of Advances in Linguistics are licensed under a Creative Commons Attribution 4.0 International License.