JOURNAL OF ADVANCES IN BIOTECHNOLOGY 2020-01-02T15:38:43+00:00 Editorial Office Open Journal Systems Micropropagation and ex situ conservation of Silene fabaria (L.) Sm. in Sibth. & Sm. subsp. domokina Greuter (Caryophyllaceae); an important endemic plant in Greece with medicinal and ornamental value 2019-04-10T07:49:47+00:00 Virginia Sarropoulou Eleni Maloupa <p><em>Silene</em> species (Caryophyllaceae) are sources of important secondary metabolites with extensive use in traditional medicine and potential applications as ornamentals. The present study was conducted to assess the regeneration potential of <em>Silene fabaria</em> subsp. <em>domokina </em>to produce massive clonal <em>in vitro</em> plants. Two experiments were conducted. The basal culture medium used was the MS. In the first experiment, the effect of 3 cytokinins; BA, KIN and 2-ip applied alone and in combination with 3 auxins; IBA, NAA and IAA was studied. In the second experiment, the effect of 3 auxins; IBA, NAA and IAA, each applied in 3 different concentrations (0.1, 0.25 and 0.5 mg/l) was studied. Shoot proliferation 100%, highest shoot proliferation rate (4.83) and shoot number (3.67) were achieved with 0.25 mg/l BA and 0.1 mg/l IAA (5 weeks). IAA at 0.5 mg/l was the most effective in stimulating shoot elongation (80.63 mm). Rooting 100% was obtained with 0.1 mg/l IBA yielding 7.3 roots 22.91 mm long (4 weeks). <em>In vitro</em> plants were successfully acclimatized with 92.31% survival rate. This study is the first micropropagation report of <em>S. fabaria</em> subsp. <em>domokina</em> that could be exploited for rapid, large-scale production and future germplasm maintenance of this valuable prioritized species-subspecies.</p> 2019-02-28T00:00:00+00:00 Copyright (c) 2019 Virginia Sarropoulou, Eleni Maloupa Therapeutic effectiveness of Hydroxyapatite Nanoparticles and Pulsed Electromagnetic Field in Osteoporosis and Cancer 2019-07-03T06:38:46+00:00 Divya Prakash Shikha S Chauhan Jitendra Behari <p>The emergence of nanotechnology has had a profound effect on many areas of healthcare and scientific research. Several studies reported the importance Hydroxyapetite Nanoparticles in the biomedical field in general, and in emerging areas such as implants, drug delivery, cancer, composites, coatings, and ceramic materials in particular. On the other hand, low level Pulsed electromagnetic field (PEMF) therapy presents several potential advantages including non-invasiveness, safety, highly influential in the fracture repair process, lack of toxicity for non-cancerous cells, and the possibility of being combined with other available therapies. It has also been observed that the combined effect of these two can accelerate the osteognic and anticancer activity in the osteoporotic and carcinoma cell lines respectively. The objective of this review is to provide a broad recount of the applications of PEMFs and Hydroxyapatite nanoparticles in osteoporosis and cancer and to then demonstrate what is further required for enhanced therapeutic outcomes.</p> 2019-02-28T00:00:00+00:00 Copyright (c) 2019 Divya Prakash, Shikha S Chauhan, Jitendra Behari Extraction and Purification of Antimicrobial Proteins from Datura Stramonium Seed 2019-05-30T09:51:11+00:00 Surayya Mustapha Muhammad Ibrahim A. Sabo Ahmed Mohammed Gumel Fatima I, Alkali <p>Resistant to antibiotics by microbes have become a major global challenge incurring economic and public health burden. Hence, research to develop new effective anti-biotics should be a major consideration to pharmaceutical industries, scientific researchers, Amanda ‘scientific researchers and world health organizations at large. This study is conducted to investigate the antimicrobial property of proteins present in the seeds of Datura stramonium. The extraction was carried out using Tris-HCL buffer prepared from 50M Tris and 0.3M NaCL, the proteins were isolated using ammonium sulphate precipitation to obtain 80% fraction. The isolated and extracted proteins sample were subjected to dialysis in which all the salt was removed and then purified using Ion-exchange chromatography. Acidic and basic fractions of the proteins obtained were subjected to SDS-PAGE electrophoresis to visualize their different molecular weight. More protein band was observed in the basic fraction between 9-45kDa. Antibacterial activities of both acidic and basic proteins were carried out using the paper disc diffusion method against clinical bacterial isolates of E. coli and Klebsiella pneumoniae. More activity was observed in basic protein with a diameter 8mm compared to the acidic fraction of 7mm in diameter.&nbsp;</p> 2019-04-17T00:00:00+00:00 Copyright (c) 2019 Surayya Mustapha Muhammad, Ibrahim A. Sabo, Ahmed Mohammed Gumel, Fatima I, Alkali Graphic Model-based Gene Regulatory Network Reconstruction using RNA Sequencing Count Data 2019-05-31T03:56:58+00:00 Liliana Lopez-Kleine Cristian Andres Gonzalez-Prieto <p>Interactions between genes, such as regulations are best represented by gene regulatory networks (GRN). These are often constructed based on gene expression data. Few methods for the construction of GRN exist for RNA sequencing count data. One of the most used methods for microarray data is based on graphical Gaussian networks. Considering that count data have different distributions, a method assuming RNA sequencing counts distribute Poisson has been proposed recently. Nevertheless, it has been argued that the most likely distribution of RNA sequencing counts is not Poisson due to overdispersion. Therefore, the negative binomial distribution is much more likely. For this distribution, no model-based method for the construction of GRN has been proposed until now. Here, we present a graphical, model-based method for the construction of GRN assuming a negative binomial distribution of the RNA sequencing count data. The R code is available under request. We used the method proposed both on simulated RNA sequencing count data and on real data. The graph is showed, and its descriptive measurements were assessed. They were found some interesting biological conclusions. We confirm that using negative binomial distribution for fitting the model is suitable because RNA sequencing data present overdispersion.</p> 2019-05-30T00:00:00+00:00 Copyright (c) 2019 Liliana Lopez-Kleine, Cristian Andres Gonzalez-Prieto Effect of The Ethylene Inhibitor “Agno3”, Vitamin B9 “Folic Acid” And Thiol Compound “GSH” On in Vitro Propagation of Sideritis Syriaca L. Subsp. Syriaca (Hellenic Mountain Tea of The Crete Island) 2019-08-10T10:42:59+00:00 Virginia Sarropoulou Eleni Maloupa <p>The aim of this research was to study the effect of the ethylene inhibitor “silver nitrate (AgNO3)” and vitamin B9 "folic acid" in different concentrations combined with cytokinin BA as well as the antioxidant thiol compound “L-glutathione reduced (GSH)” in different concentrations simultaneously with auxins (IBA+NAA) on micropropagation efficiency of the endemic <em>Sideritis syriaca </em>L. subsp. <em>syriaca</em> (Hellenic mountain tea of Crete/ Malotira) using shoot tip explants. The culture medium used was the MS supplemented with 30 g/l sucrose. The simultaneous application 5 ?M AgNO<sub>3</sub> + 2.2 ?M BA promoted best the initial shoot induction stage exhibiting 4.5 shoots/ explant and 100% shoot multiplication (5 weeks). Folic acid applied at 0.25 mg/l in combination with 0.5 mg/l BA exhibited the highest shoot multiplication percentage (90%) (4 weeks). GSH at 10 ?M with 10.7 ?M NAA + 4.92 ?M IBA gave the greatest root length (13.68 mm), at 25 ?M caused a 3-fold increase in rooting (90%) and 250 ?M GSH raised by 20% shoot multiplication (80%). An 89% final survival rate of rooted microplants to <em>ex vitro </em>unheated greenhouse conditions was recorded within 8 weeks period during mid-late spring. Thus, the acclimatization and hardening process was successfully completed.</p> 2019-08-07T00:00:00+00:00 Copyright (c) 2019 Virginia Sarropoulou, Eleni Maloupa The Induction of High-Frequency Callus with an Elite Cotton (Gossypium Hirsutum L.) from Côte d'Ivoire 2020-01-02T15:38:43+00:00 Affoué Sylvie Rachelle N'GUESSAN Koutouan AYOLIE Sopie Edwige Salomé YAPO Yao Kouakou Francois KONAN Achi Laurent N’CHO Kouakou Désiré N’GORAN Dramane KONE Yatty Justin KOUADIO Tanoh Hilaire KOUAKOU <p>In cotton, factors such as carbon sources, hormonal combination, and type of explant influence the responses to callus induction. In this study callus induction was optimized to get high-frequency callus in cotton. Different carbon sources were tested, and among them, glucose at 3% was found to be the best carbohydrate in callus induction. Culture medium supplemented with 2,4-D (0.1 mg.L<sup>-1</sup>) and KIN (0.5 mg.L<sup>-1</sup>) gave a high callogenesis response, unlike other hormonal combinations. With explants, hypocotyl&nbsp;(72.93%) appeared easier to induce callus than cotyledon (48.75%) following root (37.5%). So, carbone sources, hormone, and their concentration affect significantly callus induction. The highest frequency of callus induction was then observed when hypocotyl explants were cultured on medium supplemented with 0.1 mg.L<sup>-1 </sup>of 2,4-D in combination with 0.5 mg.L<sup>-1 </sup>of KIN and 3% glucose. The induced-callus obtained were friable and of high quality. The other types of combinations resulted in the formation of compact callus with sometimes roots emerging from it. The present study may provide an efficient protocol to improve the callogenesis responses by adjusting the carbohydrates, the hormonal regime, and the type of explant to be cultured for a high-frequency callus induction of the Y331B-R5 cotton cultivar.</p> <p>&nbsp;</p> 2019-11-07T00:00:00+00:00 Copyright (c) 2019 Affoué Sylvie Rachelle N'GUESSAN, Koutouan AYOLIE, Sopie Edwige Salomé YAPO, Yao Kouakou Francois KONAN, Achi Laurent N’CHO, Kouakou Désiré N’GORAN, Dramane KONE, Yatty Justin KOUADIO, Tanoh Hilaire KOUAKOU Editorial, Volume 8 (2019) 2019-12-27T08:57:35+00:00 Dr. James John <p>On behalf of the Editorial Board, it is with great pride and sincere privilege that I am writing this message to present the volume 8 (2019) of <em>the&nbsp;JOURNAL OF ADVANCES IN BIOTECHNOLOGY</em>. The issue comes from a long process, and we took all the necessary steps to make it a high-caliber scientific publication. We are relying on the collaboration of all our Editors, reviewers, and contributors to make it a contemporary, lively, and relevant publication.</p> 2019-12-27T00:00:00+00:00 Copyright (c) 0 Annual Reviewer Acknowledgement 2019-12-27T05:30:11+00:00 Dr. James John <p>The Editorial team of the journal would like to thank the reviewers for their work in refereeing manuscripts during 2019</p> 2019-12-27T00:00:00+00:00 Copyright (c) 0