JOURNAL OF ADVANCES IN BIOTECHNOLOGY 2022-06-08T05:26:27+00:00 Editorial Office Open Journal Systems Response of haustorium tissues and coconut water in somatic embryos induction for the coconut palm (Cocos nucifera L.) variety PB121 2022-02-10T17:20:16+00:00 Arnaud AGBIDINOUKOUN Euloge Rimson Somakpe Jerome Anani Houngue Serge S. HouƩdjissin Corneille Ahanhanzo <p>The coconut tree (<em>Cocos nucifera </em>L.) is a fruit plant that contributes significantly to improved nutrition, food security, job creation, and household income in Benin. However, its production is suffering from the unavailability of certified seedlings. The present work aimed to optimize the propagation of coconut trees through the somatic embryogenesis technique. Zygotic embryos were cultured <em>in vitro</em> on Y3 medium supplemented with 0.7% Agar; 2.5 g/l activated charcoal, 5% sucrose to obtain haustorium, and the radicle explants for somatic embryogenesis. Three months after, callus and somatic embryos were induced from haustorium and radicle on medium Y3 supplemented with different doses of 2,4-D (0.3 and 0.35 mM) and coconut water (0, 50, 100 and 150 ml/l). 80% of callus was induced of induced with haustorium explant on Y3 medium supplemented with 0.7 mM 2,4-D. The combination of 2,4-D and coconut water resulted in the highest average number of somatic embryos with 59 and 63 embryos obtained respectively on Y3 medium enriched with 150 ml/l coconut water and supplemented with 0.3 mM and 0.35 mM 2,4-D. Using haustorium explant for mass propagation through somatic embryogenesis remains an exploring way for <em>in vitro</em> seedling of coconuts.</p> 2022-07-01T00:00:00+00:00 Copyright (c) 2022 Arnaud AGBIDINOUKOUN, Euloge Rimson Somakpe , Jerome Anani Houngue , Serge S. HouƩdjissin, Corneille Ahanhanzo Comparative study on oyster mushroom grown on composited substrate: The effect on yield, growing period and fruiting body size 2022-03-07T05:29:04+00:00 Fimanekeni Ndaitavela Shivute <p>Cultivation of the oyster mushroom <em>on horse manure and wheat straw compost</em> without nutrient supplementation was investigated. The growing, yield and fruiting body size effects on open trays and substrate bags were determined. Incubation and fruiting period on trays and inoculated bags were compared. The bagged compost yielded higher mushroom growth rate and yield than the tray compost. The fruiting bodies of the mushroom on trays were smaller, pile and thinner as compared to the mushrooms on the bags, which were bigger, fresh and strong. However, it was found that when oyster mushroom are grown on trays, the yield decrease, there is less moisture in the tray and substrate is exposed to heat, the pin head dries as they develop and those that succeed to grown further will grow as thin with a little head due to lack of oxygen. Comparing compost in bags with compost substrate in trays, bags yielded about 20% more mushrooms than trays under the same cultivation conditions. Conversely, the incubation period of compost in bags took longer, as compared to the incubation of compost in trays. Trays gave their first flash 10% earlier than the bags.</p> 2022-06-08T00:00:00+00:00 Copyright (c) 2022 Fimanekeni Ndaitavela Shivute