JOURNAL OF ADVANCES IN BIOTECHNOLOGY

Comparative study on oyster mushroom grown on composited substrate: The effect on yield, growing period and fruiting body size

Fimanekeni Ndaitavela Shivute — 2022-06-08

Cultivation of the oyster mushroom on horse manure and wheat straw compost without nutrient supplementation was investigated. The growing, yield and fruiting body size effects on open trays and substrate bags were determined. Incubation and fruiting period on trays and inoculated bags were compared. The bagged compost yielded higher mushroom growth rate and yield than the tray compost. The fruiting bodies of the mushroom on trays were smaller, pile and thinner as compared to the mushrooms on the bags, which were bigger, fresh and strong. However, it was found that when oyster mushroom are grown on trays, the yield decrease, there is less moisture in the tray and substrate is exposed to heat, the pin head dries as they develop and those that succeed to grown further will grow as thin with a little head due to lack of oxygen. Comparing compost in bags with compost substrate in trays, bags yielded about 20% more mushrooms than trays under the same cultivation conditions. Conversely, the incubation period of compost in bags took longer, as compared to the incubation of compost in trays. Trays gave their first flash 10% earlier than the bags.

Anti-Inflammatory Activity of Aqueous Extract of Stem Bark of Cassia Sieberiana (Caesalpiniaceae)

SORO Tianga Yaya — 2022-12-31

Cassia sieberiana is a tree of 8 to 10 meters in height, used to treat various diseases including malaria, dysmenorrhea and many others. Our objective is the scientific valorisation of Cassia sieberiana, a plant used in therapy in Ivory Coast, by evaluating the anti-inflammatory activity of the bark of the root of Cassia sieberiana. To do this, the phytochemical study was carried out in order to determine the main chemical constituents with therapeutic potential, then the acute toxicity by gavage and intraperitoneal injection were carried out and finally the anti-inflammatory activity was verified.

The phytochemical study revealed the presence of sterols, polyterpenes, polyphenols, flavonoids, catechic tannins, saponosides and alkaloids. As for the toxicity study, it allowed us to determine by per os route an LD50 > 5000 mg/kg PC, and by IP an LD50 = 524, 807 mg/kg PC graphically and by calculation an LD50 = 400 mg/kg PC. The anti-inflammatory activity of the aqueous extract of Cassia sieberiana at a dose of 200 mg/kg CP is higher than that of diclofenac at a dose of 10 mg/kg CP from the 3rd to the 6th hour of the experiment. Cassia sieberiana is non-toxic by oral administration but toxic by intraperitoneal injection. Cassia sieberiana also has anti-inflammatory activity.

Haemoglobin and white blood cells (WBC) as key haematological indicators of malaria infection in a population in Côte d'Ivoire

KASSI Bohoussou Georges — 2022-10-07

Malaria is the main cause of consultation and hospitalisation in health centres and the leading cause of morbidity and mortality in Côte d'Ivoire. To reduce malaria-related deaths, rapid diagnosis and treatment strategies should be adopted. The aim of this study was to determine the relationship between parasite density and selected haematological parameters in infected patients in cities located in southern Côte d'Ivoire. Blood samples were collected in Jacqueville and Tiassalé. The different haematological parameters were identified by an automated haematological analyser. The coefficient of determination (R2) was determined to show the proportion of variation in parasite density and each haematological parameter. A total of 69 patients were sampled. A negative correlation was observed between parasite density and haemoglobin in the general population, in Jacqueville and in Tiassalé. However, this correlation is significant in the general population (p1 = 0.046; Confidence Interval (CI= [-0.0047; -0.00010]) with a correlation intensity (r) different from zero (p2 = 0.05) and in Jacqueville (p1 = 0. 041; CI= [-0.0025; -0.00012]) with a correlation intensity (r) different from zero (p2 = 0.04). In Tiassalé the correlation was not significant. Haemoglobin and WBC can be considered as key haematological indicators of malaria infection in the study population.

Response of haustorium tissues and coconut water in somatic embryos induction for the coconut palm (Cocos nucifera L.) variety PB121

Arnaud AGBIDINOUKOUN — 2022-07-01

The coconut tree (Cocos nucifera L.) is a fruit plant that contributes significantly to improved nutrition, food security, job creation, and household income in Benin. However, its production is suffering from the unavailability of certified seedlings. The present work aimed to optimize the propagation of coconut trees through the somatic embryogenesis technique. Zygotic embryos were cultured in vitro on Y3 medium supplemented with 0.7% Agar; 2.5 g/l activated charcoal, 5% sucrose to obtain haustorium, and the radicle explants for somatic embryogenesis. Three months after, callus and somatic embryos were induced from haustorium and radicle on medium Y3 supplemented with different doses of 2,4-D (0.3 and 0.35 mM) and coconut water (0, 50, 100 and 150 ml/l). 80% of callus was induced of induced with haustorium explant on Y3 medium supplemented with 0.7 mM 2,4-D. The combination of 2,4-D and coconut water resulted in the highest average number of somatic embryos with 59 and 63 embryos obtained respectively on Y3 medium enriched with 150 ml/l coconut water and supplemented with 0.3 mM and 0.35 mM 2,4-D. Using haustorium explant for mass propagation through somatic embryogenesis remains an exploring way for in vitro seedling of coconuts.