A 100% Water Mobile Phase HPLC-PDA Analysis of Selected Neonicotinoid Insecticides
DOI:
https://doi.org/10.24297/jac.v10i9.878Keywords:
Internal harmonized analytical method, Acetamiprid, Imidacloprid, High-Performance Liquid Chromatography, Photo-diode arrayAbstract
This paper describes a reserved-phase HPLC method for detecting frequently-used neonicotinoid insecticides, acetamiprid (ATP) and imidacloprid (ICP), using an isocratic 100 % water mobile phase. Chromatographic separations were performed an Inertsil® WP300 C4 with water mobile phase and a photodiode-array detector. The total run time was < 7 min. The system suitability was well within the international acceptance criteria.  The detection limits were 0.013 μg ml-1 for ATP and 0.015 μg ml-1 for ICP, respectively. A harmless HPLC method for simultaneous detecting ATP and ICP was developed and may be further applied to the quantification in foods.
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