Micropropagation of Elite Genotypes of Castanea Sativa (MILL.)

Authors

  • Filippos A. (Phil) Aravanopoulos Aristotle University of Thessaloniki, Thessaloniki, PO Box 238, GR54124.
  • Dorothy T. Tchatchoua Animal Husbandry and Derived Products, Higher institute of the Sahel (ISS), University of Maroua.
  • Evangelos Barbas Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, PO Box 238, GR54124.

DOI:

https://doi.org/10.24297/jbt.v3i2.1679

Keywords:

European chestnut, plus-trees, MS media, rooting media, multiplication traits, clonal testing.

Abstract

Sprouted buds of eight mature plus-trees of Castanea sativa were collected from two natural populations (Mt. Paiko and Mt. Hortiatis, Greece). Explants were cultured on Murashige and Skoog (MS) medium (three concentrations of macro and micro-elements) combined with three concentrations of BAP (6-Benzylaminopurine). Results were evaluated based on three multiplication traits: (a) number of shoots produced, (b) length of the longest shoot segments and (c) percentage of responsive explants. A high genotype-dependant variation for the number of shoots produced per explant was detected. MS medium at full salt strength and 0.2 mg l-1 BAP provided the best results based on the multiplication traits measured. Elite mature tree P-2 proved to be highly favourable for multiplication. Rooting was attempted on ½ MS medium at five different NAA concentrations. Successful rooting (56%) was obtained by using 0.50 mg l-1 NAA in vermiculite medium after eight weeks. These results indicate that the P-2 genotype can be micropropagated from the protocol developed for ornamental purposes, clonal testing and tree improvement applications.

Downloads

Download data is not yet available.

Author Biographies

Filippos A. (Phil) Aravanopoulos, Aristotle University of Thessaloniki, Thessaloniki, PO Box 238, GR54124.

Professor of forest Genetics and Tree Breeding, Faculty of Agriculture, Forestry and Natural Environment.

Dorothy T. Tchatchoua, Animal Husbandry and Derived Products, Higher institute of the Sahel (ISS), University of Maroua.

Department of Agriculture.

Evangelos Barbas, Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, PO Box 238, GR54124.

Laboratory of Forest Genetics and Tree Breeding, Faculty of Agriculture.

Downloads

Published

2014-06-21

How to Cite

Aravanopoulos, F. A. (Phil), Tchatchoua, D. T., & Barbas, E. (2014). Micropropagation of Elite Genotypes of Castanea Sativa (MILL.). JOURNAL OF ADVANCES IN BIOTECHNOLOGY, 3(2), 200–209. https://doi.org/10.24297/jbt.v3i2.1679

Issue

Vol. 3 No. 2

Section

Articles

License

Creative Commons License All articles published in Journal of Advances in Linguistics are licensed under a Creative Commons Attribution 4.0 International License.