Population Structure and Genetic Diversity of the Medicinal and Aromatic Plant Cunila spicata Benth. (Lamiaceae) Based on Inter-Simple Sequence Repeat (ISSR) Markers

Authors

  • Ana Paula Longaray Delamare Institute of Biotechnology, University of Caxias do Sul, Box 1132, Caxias do Sul 95001-970
  • Sergio Echeverrigaray Institute of Biotechnology, University of Caxias do Sul, Box 1132, Caxias do Sul 95001-970
  • Marcos Albuquerque Institute of Biotechnology, University of Caxias do Sul, Box 1132, Caxias do Sul 95001-970
  • Jucimar Zacaria Institute of Biotechnology, University of Caxias do Sul, Box 1132, Caxias do Sul 95001-970

DOI:

https://doi.org/10.24297/jbt.v5i2.1568

Keywords:

Cunila spicata, ISSR, genetic diversity, conservation

Abstract

Cunila spicata is an endangered aromatic and medicinal plant of South Brazil. In the present paper, the ISSR technique was employed to study the intra- and inter-population genetic diversity of this species. Nine primers generated a total of 109 amplification products, most of which were polymorphics. Low genetic diversity at population level (HE= 0.053) and species level (HT=0.196), and high differentiation among populations (GST= 0.727) were detected in C. spicata. The genetic diversity, low estimated genetic flow and absence of correlation between genetic distances, geographic distances and chemical composition, indicates that genetic drift and inbreeding may be the main factors involved in the genetic structure of C. spicata. Based on these findings, strategies are proposed for the genetic conservation and management of this species.

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Author Biographies

Ana Paula Longaray Delamare, Institute of Biotechnology, University of Caxias do Sul, Box 1132, Caxias do Sul 95001-970

Institute of Biotechnology

Sergio Echeverrigaray, Institute of Biotechnology, University of Caxias do Sul, Box 1132, Caxias do Sul 95001-970

Institute of Biotechnology

Marcos Albuquerque, Institute of Biotechnology, University of Caxias do Sul, Box 1132, Caxias do Sul 95001-970

Institute of Biotechnology

Jucimar Zacaria, Institute of Biotechnology, University of Caxias do Sul, Box 1132, Caxias do Sul 95001-970

Institute of Biotechnology

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Published

2016-02-23

How to Cite

Delamare, A. P. L., Echeverrigaray, S., Albuquerque, M., & Zacaria, J. (2016). Population Structure and Genetic Diversity of the Medicinal and Aromatic Plant Cunila spicata Benth. (Lamiaceae) Based on Inter-Simple Sequence Repeat (ISSR) Markers. JOURNAL OF ADVANCES IN BIOTECHNOLOGY, 5(2), 652–658. https://doi.org/10.24297/jbt.v5i2.1568

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