Detection of luxS gene expression under stressing factors for biofilm formation by Propionibacterium acidipropionici and Propionibacterium freudenreichii

Authors

  • Danitza Xiomara Romero Instituto de Investigaciones Fármaco Bioquímicas – IIFB, Universidad Mayor de San Andrés. La Paz, Bolivia.
  • Oscar Víctor Cárdenas Instituto de Investigaciones Fármaco Bioquímicas – IIFB, Universidad Mayor de San Andrés. La Paz, Bolivia
  • Víctor Hugo Cavero Olguin Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden.
  • María Teresa Álvarez Aliaga Instituto de Investigaciones Fármaco Bioquímicas – IIFB, Universidad Mayor de San Andrés. La Paz, Bolivia.

DOI:

https://doi.org/10.24297/jbt.v5i2.1558

Keywords:

Biofilm, FISH expression modified, P. freudenreichii subsp, shermanii DSM 4902T, P, acidipropionici DSM 4900T, stressing factors and luxS.

Abstract

Gene expression constitutes an important role in cellular communication, setting mechanisms for biofilm formation. Genes can be used as molecular markers to monitor viability, stability and maintenance of biofilm eg. in biofilm reactors, bioremediation and biotransformation frequently under stressing conditions to enhance or limit the biofilm formation.
In the present study, no pathogenic microorganisms of industrial interest were used. Propionibacterium freudenreichiisubsp. shermanii DSM 4902T and P. acidipropionici DSM 4900Tstrains cannot produce biofilm in culture conditions previously reported. In this regard, chemical culture conditions were modified to stimulate biofilm formation in both strains and determine that under stressing conditions such as 0.6 M NaCl, 1.8 M glucose and 10 gL-1 yeast extract both Propionibacterium produce biofilm. Finally, luxS expression was identified in biofilm of both strains by modified fluorescent in situ hybridization expression (FISH expression).

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Author Biography

Víctor Hugo Cavero Olguin, Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden.

Department of Biotechnology

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Published

2015-11-16

How to Cite

Romero, D. X., Cárdenas, O. V., Olguin, V. H. C., & Aliaga, M. T. Álvarez. (2015). Detection of luxS gene expression under stressing factors for biofilm formation by Propionibacterium acidipropionici and Propionibacterium freudenreichii. JOURNAL OF ADVANCES IN BIOTECHNOLOGY, 5(2), 604–613. https://doi.org/10.24297/jbt.v5i2.1558

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