Preparation, Characterization and Antibacterial Activity of Pomegranate Peel Extract and Chitosan-Silver Nanoparticles Using Electrochemical Method.


  • Afaf Sarhan Faculty of Science, Mansoura University
  • M. I. Abdel Hamid Faculty of Science, Mansoura University
  • Sabrien A. Omar faculty of Agriculture, Mansoura University
  • R. Hanie Faculty of Agriculture, Mansoura University



Chitosan, Pomegranate Peel Extract, Silver Nanoparticles, Antibacterial Activity, Electrochemical Method


The present work involves the development of chitosan-pomegranate peel extract and silver nanoparticles (Cs-PPE-AgNPs) using electrochemical process followed by UV irradiation reduction. Formation of silver nanoparticles characterize by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential thermal analysis (DTA), SEM, Swelling and thermal gravimetric analysis (TGA). The obtain nanoparticles average size was 34.6 nm. UV Spectroscopy show new peak at 405nm indicate the formation of AgNPs particles. Cs-PPE and Cs-PPE-AgNPs also demon started a relativity high antibacterial against Escherichia coli and Staphylococcus aureus bacteria.


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Author Biographies

Afaf Sarhan, Faculty of Science, Mansoura University

Department, Faculty of Science.

M. I. Abdel Hamid, Faculty of Science, Mansoura University

Department, Faculty of Science, Mansoura University.

Sabrien A. Omar, faculty of Agriculture, Mansoura University

Department, faculty of Agriculture.

R. Hanie, Faculty of Agriculture, Mansoura University

Department, Faculty of Agriculture.


Acosta, N., Sánchez, E., Calderón, L., Cordoba-Diaz, M., Cordoba-Diaz, D., Dom, S., & Heras, Á. (2015). Physical stability studies of semi-solid formulations from natural compounds loaded with chitosan microspheres. Marine drugs, 13(9), 5901-5919.

Akhtar, S., Ismail, T., Fraternale, D., & Sestili, P. (2015). Pomegranate peel and peel extracts: Chemistry and food features. Food chemistry, 174, 417-425.

Bui, V. K. H., Park, D., & Lee, Y.-C. (2017). Chitosan combined with ZnO, TiO2 and Ag nanoparticles for antimicrobial wound healing applications: a mini review of the research trends. Polymers, 9(1), 21.

Cao, X., Cheng, C., Ma, Y., & Zhao, C. (2010). Preparation of silver nanoparticles with antimicrobial activities and the researches of their biocompatibilities. Journal of Materials Science: Materials in Medicine, 21(10), 2861-2868.

De Leonardis, A., Aretini, A., Alfano, G., Macciola, V., & Ranalli, G. (2008). Isolation of a hydroxytyrosol-rich extract from olive leaves (Olea Europaea L.) and evaluation of its antioxidant properties and bioactivity. European Food Research and Technology, 226(4), 653-659.

Fares, R., Bazzi, S., Baydoun, S. E., & Abdel-Massih, R. M. (2011). The antioxidant and anti-proliferative activity of the Lebanese Olea europaea extract. Plant Foods for Human Nutrition, 66(1), 58-63.

Francis, L., Balakrishnan, A., Sanosh, K., & Marsano, E. (2010). Hydroxy propyl cellulose capped silver nanoparticles produced by simple dialysis process. Materials Research Bulletin, 45(8), 989-992.

Goldsmith, C. D., Vuong, Q. V., Sadeqzadeh, E., Stathopoulos, C. E., Roach, P. D., & Scarlett, C. J. (2015). Phytochemical properties and anti-proliferative activity of Olea europaea L. leaf extracts against pancreatic cancer cells. Molecules, 20(7), 12992-13004.

Goldsmith, C. D., Vuong, Q. V., Stathopoulos, C. E., Roach, P. D., & Scarlett, C. J. (2014). Optimization of the aqueous extraction of phenolic compounds from olive leaves. Antioxidants, 3(4), 700-712.

Haerudin, H., Pramono, A. W., Kusuma, D. S., Jenie, A., Voelcker, N. H., & Gibson, C. (2010). Preparation and characterization of chitosan/ontmorillonite MMT nanocomposite systems University of Indonesia].

Ismail, E. H., Khalil, M. M., Al Seif, F. A., El-Magdoub, F., Bent, A., Rahman, A., & Al, U. (2014). Biosynthesis of gold nanoparticles using extract of grape (Vitis vinifera) leaves and seeds. Prog Nanotechnol Nanomater, 3, 1-12.

Joshi, J., Kalainathan, S., Joshi, M., & Parikh, K. (2020). Crystal growth, spectroscopic, second and third order nonlinear optical spectroscopic studies of L-phenylalanine doped ammonium dihydrogen phosphate single crystals. Arabian Journal of Chemistry.

Kaur, P., Choudhary, A., & Thakur, R. (2013). Synthesis of chitosan-silver nanocomposites and their antibacterial activity. Int J Sci Eng Res, 4(4), 869.

Liu, J., Lu, J.-f., Kan, J., Tang, Y.-q., & Jin, C.-h. (2013). Preparation, characterization and antioxidant activity of phenolic acids grafted carboxymethyl chitosan. International Journal of Biological Macromolecules, 62, 85-93.

Loo, Y. Y., Chieng, B. W., Nishibuchi, M., & Radu, S. (2012). Synthesis of silver nanoparticles by using tea leaf extract from Camellia sinensis. International journal of nanomedicine, 7, 4263.

López-Mata, M. A., Ruiz-Cruz, S., Silva-Beltrán, N. P., Ornelas-Paz, J. D. J., Zamudio-Flores, P. B., & Burruel-Ibarra, S. E. (2013). Physicochemical, antimicrobial and antioxidant properties of chitosan films incorporated with carvacrol. Molecules, 18(11), 13735-13753.

Morsi, M., Oraby, A., Elshahawy, A., & El-Hady, R. A. (2019). Preparation, structural analysis, morphological investigation and electrical properties of gold nanoparticles filled polyvinyl alcohol/carboxymethyl cellulose blend. Journal of Materials Research and Technology, 8(6), 5996-6010.

Neto, C. d. T., Giacometti, J., Job, A., Ferreira, F., Fonseca, J., & Pereira, M. (2005). Thermal analysis of chitosan based networks. Carbohydrate polymers, 62(2), 97-103.

Papathanassiou, A. (1999). Thermal depolarization studies in leukolite (polycrystalline magnesite, MgCO3). Journal of Physics and Chemistry of Solids, 60(3), 407-414.

Pereira, F. S., da Silva Agostini, D. L., Job, A. E., & González, E. R. P. (2013). Thermal studies of chitin–chitosan derivatives. Journal of thermal analysis and calorimetry, 114(1), 321-327.

Qi, L., Xu, Z., Jiang, X., Hu, C., & Zou, X. (2004). Preparation and antibacterial activity of chitosan nanoparticles. Carbohydrate research, 339(16), 2693-2700.

Qin, Y.-Y., Zhang, Z.-H., Li, L., Yuan, M.-L., Fan, J., & Zhao, T.-R. (2015). Physio-mechanical properties of an active chitosan film incorporated with montmorillonite and natural antioxidants extracted from pomegranate rind. Journal of food science and technology, 52(3), 1471-1479.

Romainor, A. N. B., Chin, S. F., Pang, S. C., & Bilung, L. M. (2014). Preparation and characterization of chitosan nanoparticles-doped cellulose films with antimicrobial property. Journal of Nanomaterials, 2014.

Silverstein, R. M., & Bassler, G. C. (1962). Spectrometric identification of organic compounds. Journal of Chemical Education, 39(11), 546.

Suntres, Z. E., Coccimiglio, J., & Alipour, M. (2015). The bioactivity and toxicological actions of carvacrol. Critical reviews in food science and nutrition, 55(3), 304-318.

Zghal, E., Namouchi, F., & Guermazi, H. (2014). Study of polarization parameters effect on dipolar relaxation in epoxy-based polymer using thermally stimulated depolarization current. The European Physical Journal-Applied Physics, 65(3).

Zhang, J., Xia, W., Liu, P., Cheng, Q., Tahi, T., Gu, W., & Li, B. (2010). Chitosan modification and pharmaceutical/biomedical applications. Marine drugs, 8(7), 1962-1987.




How to Cite

Sarhan, A., Hamid, M. I. A. ., Omar, S. A. ., & Hanie, R. . (2020). Preparation, Characterization and Antibacterial Activity of Pomegranate Peel Extract and Chitosan-Silver Nanoparticles Using Electrochemical Method. JOURNAL OF ADVANCES IN PHYSICS, 17, 222–244.