Molecularly Imprinted Dispersive-Based Extraction Method for Melamine in Milk (MIDE HPLC DAD)

Authors

  • Leandro Figueiredo LEPABE – Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465, Porto
  • Lúcia Santos LEPABE – Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465, Porto
  • Arminda Alves LEPABE – Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465, Porto

DOI:

https://doi.org/10.24297/jac.v10i6.882

Keywords:

Melamine, molecularly imprinted dispersive extraction, molecularly imprinted polymer.

Abstract

An easy and selective molecularly imprinted dispersive extraction method (MIDE) coupled with HPLC-DAD was developed for melamine´s analysis in commercial milk samples.  The molecularly imprinted polymer (MIP) was synthetized by suspension polymerisation with melamine as template, while methacrylic acid, ethylene glycol dimethacrylate and chloroform were used as monomer, cross-linker and porogenic solvent, respectively.  The analytical methodology was validated presenting a limit of detection of 0.6 mg/kg. The recovery percentage in spiked milk samples ranged from 92.9% to 102.0% with an intermediate precision of 5.0%. The analysis of real samples with MIDE revealed melamine´s presence in 20% of the cases with a maximum concentration of 9.3 ± 0.3 mg/kg, which exceeds the maximum recommended level of 2.5 mg/kg.

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Published

2014-07-03

How to Cite

Figueiredo, L., Santos, L., & Alves, A. (2014). Molecularly Imprinted Dispersive-Based Extraction Method for Melamine in Milk (MIDE HPLC DAD). JOURNAL OF ADVANCES IN CHEMISTRY, 10(6), 2775–2785. https://doi.org/10.24297/jac.v10i6.882

Issue

Vol. 10 No. 6 (2014)

Section

Articles

License

Creative Commons License All articles published in Journal of Advances in Linguistics are licensed under a Creative Commons Attribution 4.0 International License.