Salt stress induced accumulation of biomolecules in groundnut genotypes


  • D. E. Jharna Professor, Department of Biochemistry and Food Analysis, 2Professor, Department of Agronomy,
  • S. C. Samanta Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh



Groundnut, Proline, Salinity stress


Salinity is one of the environmental limiting factors in agricultural production. The aim of this study was to find out one of more salt tolerant groundnut genotypes through monitoring the growth and changes in biomolecules under salt stress condition. Purposively four groundnut genotypes, including a Traditional variety, Zhingabadam, Binachinabadam-1 and Dacca-1 were grown under three salinity levels viz. 0, 3 and 5 dSm-1. The experiment was laid out in two factorial completely randomized design with three replications. This experiment was done in soil based pot culture up to 40 days. Increasing salt concentration drastically reduced all the growth parameters, and increase proline and sugar content of leaf. Among the varieties Traditional variety, Zhingabadam and Dacca-1 had statistically similar shoot and root dry weight. The leaves of the Traditional variety contain the highest amount of proline of 14.52 and 36.24 mg/100g fresh leaves in 3 and 5 dS/m salinity, respectively which was 236 and 737 % higher than that of respective control. At EC of 3 and 5 dS/m, the variety Binachinabadam-1 was appeared to be susceptible, having an increase of 6 and 113% proline content over the respective control. Based on the shoot dry weight, root dry weight, proline content, total sugar, reducing sugar and relative water content, the Traditional variety was strongly recommended to be grown in the coastal salt affected soils. The Zhingabadam and Dacca-1 variety also could be recommended as they had comparable performance of the Traditional variety.


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Aazami, M. A., Torabi, M. and Jalili, E. (2010). In vitro response of promising tomato genotypes for tolerance to osmotic stress. Afr. J. Biotechnol. 9(26): 4014- 4017.

Ashraf, M., Karim, F. and Rasul, E. (2002). Interactive effects of gibberellic acid (GA3) and salt stress on growth, ion accumulation and photosynthetic capacity of two spring wheat (Triticumaestivum L.) cultivars differing in salt tolerance. Plant Growth Regul. 36: 49-59.

Azad, M. A. K., Alam, M. S. and Hamid, M. A. (2013). Modification of Salt Tolerance Level in Groundnut ( Arachis hypogaea l.) through Induced Mutation. Legume Res. 36(3): 224 – 233.

Bates, L. S., Waldren, R. P., and Tear, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39: 205-7.

Dubey, R. S. and Singh, A. K. (1999). Salinity induces accumulation of soluble sugars and alters the activity of sugar metabolising enzymes in rice plants. Biologia Plantarum. 42: 233-239.

Dubois, M. and Gilles, K. A. (1956). Colorimetric method for determination of sugars and related substances. Annals of Chemistry. 3: 350-354.

Ghassemi, F., Jakeman, A. J., and Nix, H. A. (1995). Salinisation of land and water resources, University of New South Wales Press, Sydney.

Ghoulam, C., Foursy, A. and Fares. K. (2002). Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environ. Exp. Bot. 47: 39-50.

Girija, C., Smith, B. N. and Swamy, P. M. (2002). Interactive effects of sodium chloride and calcium chloride on the accumulation of proline and glycine betaine in peanut (Arachis hypogaea L.), Environ. Expl. Bot. 47: 1-10.

Gounipalli Veeranagamallaiah. (2013). Effect of salt stress on osmolyte accumulation in two groundnut cultivars (Arachis hypogaea L.) with contrasting salt tolerance. African Journal of Plant Science. 7(12):586-592

Karsten, H. D. and MacAdam, J. W. (2001). Effect of drought on growth carbohydrates and soil water used by Perennial Rye grass, Tall Fescue, and white clover, Crop Sci. 41:156- 166.

Lydia, A., Shtereva, Roumiana, D., Vassilevska-Ivanova and Karceva, T. V. (2015). Effect Of Salt Stress On Some Sweet Corn (Zea Mays L. Var. Saccharata) Genotypes. Research Journal of Agriculture and Forestry Sciences. 1(11): 1-8.

Mata, C. G., Lamattina, L. L. (2001). Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Plant physiology. 126: 1196-1204.

Miller, G. L. (1972). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 31: 426-428.

Munns, R. and James, R. A. (2003). Screening methods for salinity tolerance: a case study with tetraploid wheat. Plant Soil. 253: 201-218

Munns, R., Goyal, S. S. and Passioura, J. (2002). Comparative physiology of salt and water stress. Plant. Cell Evn. 25: 239-250.

Muthukumarasamy, M. and Panneerselvam, R. (1997). Amelioration of NaCl stress by triadimefon in peanut seedlings, Plant Growth Regul. 22: 157-162.

Mwai, G. N. (2001). Growth response of Spiderplant (Cleome gynandra L.) to salinity. Maseno University, Kenya. MSc thesis.

Netondo, G. W. (1999). The use of physiological parameters in screening for salt tolerance in sorghum {Sorghum bicolor L Moench} varieties grown in Kenya. Moi University, Kenya. PhD thesis

Nithila, S., Durga Devi, D., Velu, G., Amutha, R. and Rangaraju, G. (2013). Physiological Evaluation of Groundnut (Arachis hypogaea L.) Varieties for Salt Tolerance and Amelioration for Salt Stress, Res. J. Agriculture and Forestry Sci. 1(11): 1-8.

Pal and Pal, A. K., (2017). Physiological Basis of Salt Tolerance in Groundnut (Arachis hypogaea L.) International Journal of Current Microbiology and Applied Sciences. 6(9): 2157-2171

Venkateswarlu, B. and Ramesh, K. (1993). Cell membrane stability and biochemical response of cultured cells of groundnut under polyethylene glycol induced water stress. Plant-science Limerick, 90 (2): 179-185.

Yoshiba, Y., Kiyosue, T., Nakhashima, K., Yamaguchi-Shinozaki K. Y. and Shinozaki, K. (1997). Regulation of levels of proline as an osmolyte in plants under water stress. Plant Cell Physiol. 38(10): 1095-1102.




How to Cite

Jharna, D. E., & Samanta, S. C. (2021). Salt stress induced accumulation of biomolecules in groundnut genotypes. JOURNAL OF ADVANCES IN AGRICULTURE, 12, 11–21.