Determination of Glibenclamide By Analytical Spectrophotometry

Authors

  • Saad Antakli Department of Chemistry, Faculty of Science, University of Aleppo, Syria
  • Leon Nejem Department of Chemistry, Faculty of Science, University of Aleppo, Syria
  • Monzer Alraii Department of Chemistry, Faculty of Science, University of Aleppo, Syria

DOI:

https://doi.org/10.24297/jac.v18i.8963

Keywords:

Derivative spectrophotometry (1DS)., Zero spectrophotometry (ZS), Glibenclamide (GB)

Abstract

Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λmax = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R2 = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively.  First derivative spectrophotometric (1DS) method was applied for the determination of (GB) in the presence (MET). (GB) was determined at 317 nm (1D317). Linearity ranges were (4 – 240) μg/mL for (GB). Regression analysis showed a good correlation coefficients R2 = 0.999914. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.60 μg/mL and 1.83 μg/mL for (GB).

The proposed zero spectrophotometry method was applied to analysis individual (GB), and the derivative (1D317) method was applied to analysis (GB) individually or combined with (MET) in Syrian trademark drugs.

The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.

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Published

2021-04-24

How to Cite

Antakli, S. ., Nejem, L. ., & Alraii, M. . (2021). Determination of Glibenclamide By Analytical Spectrophotometry. JOURNAL OF ADVANCES IN CHEMISTRY, 18, 40–48. https://doi.org/10.24297/jac.v18i.8963

Issue

Vol. 18 (2021)

Section

Articles

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Copyright (c) 2021 Saad Antakli, Leon Nejem, Monzer Alraii

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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