Determination of Glibenclamide By Analytical Spectrophotometry
Keywords:Derivative spectrophotometry (1DS)., Zero spectrophotometry (ZS), Glibenclamide (GB)
Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λmax = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R2 = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively. First derivative spectrophotometric (1DS) method was applied for the determination of (GB) in the presence (MET). (GB) was determined at 317 nm (1D317). Linearity ranges were (4 – 240) μg/mL for (GB). Regression analysis showed a good correlation coefficients R2 = 0.999914. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.60 μg/mL and 1.83 μg/mL for (GB).
The proposed zero spectrophotometry method was applied to analysis individual (GB), and the derivative (1D317) method was applied to analysis (GB) individually or combined with (MET) in Syrian trademark drugs.
The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.
Eapen C, Prasanth V, Rai A. Development of UV Spectrometric Method of Glibenclamide (Glyburide) in Bulk and Pharmaceutical Formulations. 2012, 4(1):356-360.
British Pharmacopoeia 2009.
Belal F, Sharaf El-Din M, Aly F, Hefnawy M, El-Awady M. Spectrophotometric Analysis of a Mixture of Glyburide and Metformin HCl in Pharmaceutical Preparations. Scholars Research Library. 2011, 3(1):53-64.
Dudhe P.B, Kamble M.C, Van S, Rajpurohit V.J, Komerwar A, Gondane S.J. Development and Validation of a Spectrophotometric Method for Glibenclamide in Bulk and Tablet Dosage Forms. International Journal of PharmTech Research. 2016, 9(2): 19-23.
Bila A, Rehman K, Sajid Hamid Akash M, Hussain K, Ibrahim M, Hussan S. Development and Validation of Analytical Method for Qualitative and Quantitative Determination of Glibenclamide in Different Brands of Tablet Dosage form Using UV-Visible Spectroscopy. Molecular and Genetic Medicine, 2013, 7(3):3-6.
Umadevi N, SudheerBabu I, Mounika CH. Spectrophotometric Method Development and Validation for The Estimation of Glibenclamide in Pure Form and in Dosage Form. International Journal of Pharmaceutical Sciences and Research ,2014, 5(11) : 4915-4918.
Seema M, KhedekarB, Amnerkar D, UV Spectrophotometric Absorption Correction Method for The Simultaneous Estimation of Pioglitazone HCl, Metformin HCl and Glibenclamide in Multicomponent Formulation. International Journal of Analytical and Bioanalytical Chemistry. 2013, 3(1):18-22.
Annapurna M, Prathyusha P, Saranya S. Development and Validation of Spectrophotometric Methods for The Determination of Glibenclamide. Acta Scientific Pharmaceutical Sciences. 2019, 3(8):110-115.
Patil M, Chilkawar N, Panchal V. Validated Simple and Sensitive UV Spectrophotometric Method for Estimation of Glibenclamide in Bulk and Pharmaceutical Formulations. World Journal of Pharmacy and Pharmaceutical Sciences. 2013, 2(5):2627-2634.
Khalaf K, Hassen P. Spectrofluorimetric Method for The Determination of Glibenclamide in Pharmaceutical Formulations. Baghdad Science Journal, 2012, 9(2): 296-300.
Mohd A.B, Swathimatyam P, Padmanabha A, Shastri N, Diwan P. Development and Validation of Glibenclamide in Nanoemulsion Formulation by Using RP-HPLC. Journal of Pharmaceutical and Biomedical Sciences. 2011, 8(8): 1-5.
Jayanthi M, Thirunavukkarasu S.V, Nagarajan V, Elangovan S, Raja S. Development and Validation of RP-HPLC Method for Determination of Glibenclamide in Pharmaceutical Dosage Forms. International Journal of Chem tech Research. 2012, 4(2): 593-601.
Prashanth S, Pradeep Kumar Y, Madhu B. Development and Validation of HPLC Method for The Determination of
Glibenclamide in Rat Serum. International Journal of Pharma and Bio Sciences. 2011, 2(1): 478-488.
Josc R, Valdes S, Esther G. Determination of Glibenclamide in Human Plasma by Solid-Phase Extraction and High-Performance Liquid Chromatography. Journal of Chromatography. 1996, 682: 364-370.
Havele S, Dhaneshwar R. Determination of Glibenclamide in Tablets by Densitometric HPTLC. Scholars Research Library. 2010, 2(4): 440-446.
Malgundkar S, saira M .Validated HPTLC Method For Simultaneous Determination of Metformin Hydrochloride and Glibenclamide in Combined Dosage Form. IOSR Journal of Pharmacy and Biological Sciences. 2014, 9(2):54-59. Pharmacopeia USP 2011.
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