In vitro Antidiabetic, anti-obesity and antioxidant proprities of Rosemary extracts

Authors

  • Manel Ben Ali Laboratory of chemistry of natural substances, University of Sfax, Faculty of Sciences of Sfax, 3000, P.B. “1171”, Sfax
  • Kais Mnafgui Laboratory of Animal Ecophysiology, University of Sfax, Faculty of Sciences of Sfax, 3052, P.B. “95”, Sfax
  • Abdelfattah Feki Laboratory of Animal Ecophysiology, University of Sfax, Faculty of Sciences of Sfax, 3052, P.B. “95”, Sfax
  • Mohamed Damak Laboratory of chemistry of natural substances, University of Sfax, Faculty of Sciences of Sfax, 3000, P.B. “1171”, Sfax
  • Noureddine Allouche Faculty of Sciences of Sfax, University of Sfax TUNISIA.

DOI:

https://doi.org/10.24297/jac.v10i2.5497

Keywords:

Rosemary, diabetes, α-amylase, lipase, essential oil, oil stabilization

Abstract

Diabetes mellitus is a serious health problem worldwide that has adverse and long-lasting consequences for individuals, families, and communities. Hence, this study sought to investigate the inhibitory potential of rosemary extracts on key-enzymes related to diabetes such as α-amylase and pancreatic lipase activities, as well as to assess their antioxidant properties in vitro. The IC50 values of Rosemary essential oil, ethyl acetate and methanolic extracts against α-amylase were 28.36, 34.11 and 30.39 µg/mL respectively, and those against pancreatic lipase were 32.25, 36.64 and 34.07 µg/mL, suggesting strong anti-diabetic and anti-obesity effects of Rosemary. The methanolic extract was found to be the highest in levels of phenolic (282.98 µgGAE/mg extract) and flavonoids (161.05 µg QE /mg extract) contents as well as in the antioxidant activity (IC50 = 15.82 µg/mL) as compared to other extracts ethyl acetate (IC50 = 32.23 µg/mL) and essential oil  (IC50 = 96.12 µg/mL).

Antioxidant efficacy of Rosemary extracts has been estimated in the stabilization of sunflower oil (SFO) at three different concentrations, i.e. 200 (SFO-200), 500 (SFO-500) and 1000 ppm (SFO-1000). Results showed the highest efficiency of SFO-1000.

The results obtained in this study demonstrated for the first time that Rosemary is a potent source of natural inhibitors of α-amylase and pancreatic lipase with powerful antioxidants proprieties that might be used in the food stabilization and the prevention of diabetes and obesity complications as a complementary pharmacological drug.

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Author Biography

Noureddine Allouche, Faculty of Sciences of Sfax, University of Sfax TUNISIA.

Department of chemistry

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Published

2014-06-30

How to Cite

Ali, M. B., Mnafgui, K., Feki, A., Damak, M., & Allouche, N. (2014). In vitro Antidiabetic, anti-obesity and antioxidant proprities of Rosemary extracts. JOURNAL OF ADVANCES IN CHEMISTRY, 10(2), 2305–2316. https://doi.org/10.24297/jac.v10i2.5497

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