Extraction-Spectrophotometric Studies on the Ion-Pairing Between Some 2,3,5-Substituted Monotetrazolium Cations and Anions Deriving from 4-(2-Thiazolylazo)resorcinol or 4-(2-Pyridylazo)resorcinol

Abstract

The ion-pairing between some 2,3,5-substituted monotetrazolium cations (T⁺) and anions deriving from 4-(2-pyridylazo)resorcinol (PAR) or 4-(2-thiazolylazo)resorcinol (TAR) was studied by water-chloroform extraction and spectrophotometry. The following tetrazolium salts (TS) were used as a source of T⁺: i) 2,3,5-triphenyl-2H-tetrazolium chloride (TTC); ii) 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H-tetrazolium bromide (MTT); iii) 3-(2-naphtyl)-2,5-diphenyl-2H-tetrazolium chloride (Tetrazolium violet, TV); and iv) 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride (INT). The spectral characteristics of the extracted species were established at different pH and TS concentration. The composition and stability of the ion-pairs were determined at pH 9, where the extraction of neutral PAR (H₂PAR) and TAR (H₂TAR) species was negligible. The results showed that the ion-pairs can be expressed with the following formulae (T⁺)(HTAR^–) (where T⁺ = TT⁺, MTT⁺, TV⁺ or INT⁺), (T⁺)(HPAR^–) (where T+ = TT⁺, MTT⁺ or TV⁺) and [(INT⁺)(HPAR^–)]₂. Relationships involving the molecular masses of the ion-pairs (M_IP) or T⁺ (M_T+) and the values of the constants of association (b) or conditional molar absorptivities (e’) were examined, namely Log b = f(Log M_IP) and e’ = f(Log M_T+). Some practical aspects concerning the investigation of metal complexes with TS-PAR/TS-TAR were discussed.