Anti-Diabetic Activity Of Aqueous Extract Of Pithecellobium Dulce Benth Fruit Peel On Streptozotocin Induced Diabetic Rats
DOI:
https://doi.org/10.24297/jac.v12i15.1920Keywords:
Pithecellobium dulce fruit peel, anti-diabetic activity, streptozotocin, glibenclamideAbstract
The decoction of the Pithecellobium dulce fruit peel has been used for the control of diabetes mellitus in the traditional method adopted by the local people of northwest region of Tamil Nadu, India. The present study was carried out to investigate the anti-diabetic potential of aqueous extract of Pithecellobium dulce fruit peel by its oral administration (200 mg/kg) to streptozotocin-induced diabetic rats. The levels of blood glucose, urine sugar, glycosylated hemoglobin, glucose-6-phosphatase, fructose-1,6-bisphosphatase, total cholesterol, triglycerides, aspartate transaminase, alanine transaminase, alkaline phosphatase and reduced glutathione were increased significantly whereas the levels of plasma insulin, hexokinase, protein, liver glycogen, reduced glutathione, superoxide dismutase, catalase and glutathione peroxidase were decreased in streptozotocin-induced diabetic rats and it was normalized after treatment of aqueous extract. Glibenclamide was used as the standard drug. These outcomes suggest that the aqueous extract possesses anti-diabetic activity and supports the traditional use of the Pithecellobium dulce fruit peel decoction as hypoglycemic agent.
Downloads
Download data is not yet available.
References
1. ADA. Diagnosis and classification of diabetes mellitus. American Diabetes Association. Diabetes Care 2009; 32: 62-67.
2. Adewole SO, Caxton-Martins EA, Ojewole JA. Protective effect of quercetin on the morphology of pancreatic b-cells of streptozotocin-treated diabetic rats. Afr J Tradit Complement Altern Med. 2007; 4(1): 64 -74.
3. Alarcon-Aguilar FJ, Roman-Ramos R, Perez-Gutierrez S, Aguilar-Contreras A, Contreras-Weber CC, Flores-Saenz JL. Study of the anti-hyperglycemic effect of plants used as antidiabetics. J Ethnopharmacol. 1998; 61: 101–10.
4. Ali MS, Azhar I, Ahmad F, Ahmad AU, Smanghani KU. Antimicrobial screening of Mimosaceous plants. Pharm Biol. 2001; 39(1): 43-46.
5. Ananthan R, Latha M, Ramkumar K, Pari L, Baskar C, Bai V. Effect of Gymnema montanum leaves on serum and tissue lipids in alloxan diabetic rats. Exp Diabetes Res. 2003; 4: 183-89.
6. Banarjee, A. Studies on alkylated resin from Pithecellobium dulce. J Indian Chem Soc. 2005; 82: 186-87.
7. Baquer NZ, Gupta D, Raju J. Regulation of metabolic pathways in liver and kidney during experimental diabetes: effects of antidiabetic compounds. Indian J Clin Bioche. 1998; 13(2): 63–80.
8. Barrera NL, Bautista BS, Bravo LL, Bermudez TK, Garcia SF, Jimenez EM, Reyes CR. Antifungal activity against postharvest fungi by extracts and compounds of Pithecellobium dulce seeds (huamuchil). Acta Horticulturae. 2003; 628(2): 761-66.
9. Bautista BS, Garcia DE, Barrera NL, Reys CR, Wilson CL. Seasonal evaluation of the postharvest fungicidal activity of powders and extracts of huamuchil (Pithecellobium dulce): Action against Botrytis cinerea, Penicillium digitatum and Rhizopus stolonifer of straw berry fruit. Postharvest Biol Technol. 2003; 29: 81-92.
10. Bhargva Krishna, P., Gupta, M.B. and Chittranjan, R. Anti inflammatory activity of Saponins and other Natural Products. Indian J Med Res. 1970; 58: 724-30.
11. Bopanna KN, Kannan J, Sushma G, Balaraman R. Antidiabetic and antihyperlipidemic effects of neem seed kernel powder on alloxan diabetic rabbits. Indian J Pharmacol. 1997; 29: 162–67.
12. Delgado VF, Lopez VHE, Valdes RS, ¬Labra B, Chagolla LA, Lopez VEJ. Isolation and properties of a Kunitz-type protein inhibitor obtained from Pithecellobium dulce seeds. J Agri Food Chem. 2004; 52(20): 6115-21.
13. Devi M, Latha P. Antidiabetic effect of methanolic extract of Decalepis hamiltonii root (Wight and Arn) in normal and alloxan induced diabetic rats. J Pharm Res. 2013; 6: 166-72.
14. Felig P, Wahren J, Sherwin R, Palaiologos G. Amino acid and protein metabolism in diabetes mellitus. Arch Int Med. 1977; 137(4): 507-13.
15. Foster LB, Dunn RT. Stable reagents for determination of serum triglycerides by colorimetric Hantzsch condensation method. Clin Chem. 1973; 18: 338-40.
16. Gancedo JM, Gancedo C. Fructose-1,6-bisphosphatase, phosphofructo kinase and glucose-6-phophate dehydrogenase from fermenting and non-fermenting yeast. Arch Microbiol. 1971; 76: 132-38.
17. Gannon MC, Nuttall FQ. Effect of feeding, fasting, and diabetes on liver glycogen synthase activity, protein, and mRNA in rats. Diabetologia. 1997; 40: 758-63.
18. Gupta BL, Nehal M, Baquer NZ. Effect of experimental diabetes on the activities of hexokinase, glucose-6-phosphate dehydrogenase and catecholamines in rat erythrocytes of different ages. Indian J Exp Biol. 1997; 35: 792–95.
19. Henry RR. Thiazolidinedione. Endocrinol Metab Clin North Am. 1997; 26: 553-73.
20. Hultcrantz R, Glaumann H, Lindberg G, Nilsson LH. Liver investigation in 149 asymptomatic patients with moderately elevated activities of serum aminotransferase. Scand J Gastroenterol. 1986; 21(1): 109-13.
21. Ikino H, Kobayashi M, Suzuki Y, Konno C. Mechanism of hypoglycemic activity of aconitan A, a glycan from Aconitum carmichaelii roots. J Ethnopharmacol. 1989: 25: 295–304.
22. Jiang ZY, Woollard ACS, Wolff SP. Hydrogen peroxide production during experimental protein glycation. FEBS Lett. 1990; 268(1): 69–71.
23. Kaleem M, Asif M, Ahmed Q, Bano B. Antidiabetic and Antioxidant activity of Annona squamosa extract in streptozotocin induced diabetic rats. Singapore Med J. 2006; 47: 670-75.
24. Kameswara Rao B, Kesavulu MM, Appa Rao C. Evaluation of antidiabetic effect of Momordica cymbalaria hook fruit in alloxan diabetic rats. Fitoterapia, 2003; 74: 7-13.
25. Khan IA, Clark AM, McChesney JD. Antifungal activity of a new triterpenoid glycoside from Pithecellobium racemosum (M.). Pharm Res. 1997; 14(3): 358-61.
26. Kim MJ, Yoo KH, Kim JH, Seo YT, Ha BW, Kho JH, Shin YG, Chung CH. Effect of pinitol on glucose metabolism and adipocytokines in uncontrolled type 2 diabetes. Diabetes Res Clin Pract. 2007; 77(Suppl 1): S247-51.
27. King EJ, Armstrong AR. Determination of serum and bile phosphatase activity. Canad Med Assoc J. 1934; 31: 56-63.
28. King J. Practical Clinical Enzymology. D van Nostrand Co, Philadelphia, London. 1965; p. 1-301.
29. Kumar PA, Haseeb A, Suryanarayana P. Elevated expression of a A- and a-B crystallins in streptozotocin-induced diabetic rat. Arch Biochem Biophys. 2005; 444: 77–83.
30. Kumar S, Boulton AJ, Beck-Nielsen H, Berthezene F, Muggeo M, Persson B. Troglitazone Study Group, Troglitazone, an insulin action enhancer, improves metabolic control in NIDDM patients. Diabetologia. 1996; 39: 701-09.
31. Larcan A, Lambert H, Laprevote-Heully MC, Delorme N. Light and electron microscopic study of hepatic lesions in the course of hyperlactatemia in diabetic patients. Diabete Metab. 1979; 5(2): 103-12.
32. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin’s Phenol reagent. J Biol Chem. 1951; 193: 265-75.
33. Megala J, Geetha A. Free radical scavenging and H+,K+-ATPase inhibition activities of P. dulce. Food Chem. 2010; 121: 1120-28.
34. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase, J Biol Chem. 1972; 247(10): 3170–75.
35. Mohamed AK, Bierhaus A, Schiekofer S, Tritschler H, Ziegler R, Nawroth PP. The role of oxidative stress and NF-ðœ…B activation in late diabetic complications. Biofactors, 1999; 10(2-3): 157–67.
36. Morales MA, Jobbagy A, Terenzi HF. Mutations affecting accumulation of glycogen in Neurospora crassa. News Lett., 1973; 20: 24-25.
37. Moron MS, Depierre JN, Mannervik B. Levels of glutathione, glutathione reductase and glutathione-S-transferase activity in rat lung and liver. Biochim Biophys Acta. 1979; 582: 67–78.
38. Mule VS, Potdar VH, Jadhav SD, Disouza J. Neuropharmacological profile of aqueous and ethanolic extracts of P. dulce leaves in mice. Res J Pharm Pharmacodyn. 2011; 3(1): 27-30.
39. Murray RK, Granner DK, Mayes PA, Rodwell VW. Harper’s Biochemistry (25th ed.). Stanford, Connecticut: Appleton and Lange, 2000, pp. 610–17.
40. Nayak SS, Pattabiraman TN. A new colorimetric method for the estimation of glycosylated haemoglobin. Clin Chim Acta. 1981; 109: 267-74.
41. Osadebe PO, Omeje EO, Uzor PF, David EK, Obiorah DC. Seasonal variation for the antidiabetic activity of Loranthus micranthus methanol extract. Asian Pac J Trop Med. 2010; 3(3): 196–99.
42. Pal PB, Pal S, Manna P, Sil PC. Traditional extract of Pithecellobium dulce fruits protects mice against CCl4 induced renal oxidative impairment and necrotic cell death. Pathophysiol. 2012; 19: 101-14.
43. Palsamy P, Subramanian S. Resveratrol, a natural phytoalexin, normalizes hyperglycemia in streptozotocin-nicotinamide induced experimental diabetic rats. Biomed Pharmacother. 2008; 62: 598–605.
44. Parekh AC, Jung DH. Cholesterol determination with ferric acetate-uranium acetate and sulfuric acid-ferrous sulfate reagents. Anal Chem. 1970; 42: 1243-47.
45. Pithayanukul R, Ruenraroengsak P, Bavovada R, Pakmanee N, Suttisri R, Saenoon S. Inhibition of Naja kaouthia venom activities by plant polyphenols. J. Ethanopharmacol. 2005; 97(3): 527-33.
46. Pushparaj P, Tan CH, Tan BKH. Effects of Averrhoa bilimli leaf extract on blood glucose and lipids in streptozotocin diabetic rats. J Ethnopharmacol. 2000; 72: 69–76.
47. Rao GN, Nagender A, Sathyanarayana A, Rao G. Preparation, chemical composition and storage studies of qumachil P. dulce aril powder. J Food Sci Technol. 2011; 48(1) 90-95.
48. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalo acetic acid, glutamic pyruvate transaminase. Am J Clin Pathol. 1957; 28: 56-63.
49. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical role as a component of glatathione peroxidase, Science. 1973; 179(4073): 588–90.
50. Saravanan G, Ponmurugan P, Senthil Kumar GP, Rajarajan T. Modulatory effect of S-allylcysteine on glucose metabolism in streptozotocin induced diabetes rats. J Func. Foods. 2009; 1: 336-40.
51. Sedlak J, Lindsay RH. Estimation of the total, protein bound, and nonprotein sulfhydryl groups in tissue with Ellmans reagent. Anal Biochem. 1968; 25: 192-205.
52. Shanmugakumaran SD, Amerjothy S, Balakrishna K. Pharmacognostical, Antibacterial and antifungal potentials of the leaf extracts of Pithecellobium dulce Benth. Phcog Mag. 2006; 72: 163-67.
53. Sharma S, Choudhary M, Bhardwaj S, Choudhary N, Rana AC. Hypoglycemic potential of alcoholic root extract of Cassia occidentalis Linn in streptozotocin induced diabetes in albino mice. Bull Fac Pharm Cairo Univ. 2014; 52: 211-17.
54. Singh M, Khatoon S, Singh S, Kumar V, Rawaat AKS, Mehrotra S. Antimicrobial screening of ethnobotanically important stem bark of medicinal plants. Pharmacog Res. 2010; 2(4): 254-57.
55. Sugumaran M, Vetrichelvan T, Darline Quine S. Free radical scavenging activity of folklore: P. dulce Benth leaves. Ethnobot Leaflets, 2008a; 12: 446-51.
56. Sugumaran M, Vetrichelvan T, Darline Quine S. Locomotor activity of leaf extracts of P. dulce Benth. Ethnobot Leaflets. 2008b; 12: 490-93.
57. Sugumaran M, Vetrichelvan T, Darline Quine, S. Anti-diabetic potential of aqueous and alcoholic leaf extracts of Pithecellobium dulce. Asian J Research Chem. 2009; 2(1): 83-85.
58. Sukantha TA, Subashini KS, Ravindran NT, Balashanmugam P. Evaluation of in vitro antioxidant and antibacterial activity of Pithecellobium dulce Benth fruit peel. Int J Curr Res. 2011; 3(11): 378-82.
59. Sukantha TA, Subashini KS, Ravindran NT. Antibacterial activity of selected medicinal plant in traditional treatment of wound infection in Southeast India. Int J Pharm Pharm Sci. 2014; 6(11): 511-13.
60. Sundarrajan T, Rajkumar T, Udhayakumar E, Arunachalam G. Hypolipidemic activity of Pithecellobium dulce in Triton Wr-1339 induced hyperlipidemic activity in rats. Int J Chem Pharm Sci. 2010; 1(2): 50-53.
61. Takahara S, Hamilton HB, Nell JV. Hypocatalasemia: a new genetic carrier state, J Clin Invest. 1960; 39: 610–619.
62. Trinder P. Determination of blood glucose using an oxidaseperoxidase system with a non-carcinogenic chromogen. J Clin Pathol. 1969; 22: 158–61.
63. Venkateswaran S, Pari L. Effect of Coccinia indica on blood glucose, insulin and hepatic key enzymes in experimental diabetes. Pharmaceut. Biol. 2002; 40: 165–70.
64. Wolff SP, Dean RT. Glucose autoxidation and protein modification. The potential role of autoxidative glycosylation in diabetes. Biochem J. 1987; 245(1): 243–50.
2. Adewole SO, Caxton-Martins EA, Ojewole JA. Protective effect of quercetin on the morphology of pancreatic b-cells of streptozotocin-treated diabetic rats. Afr J Tradit Complement Altern Med. 2007; 4(1): 64 -74.
3. Alarcon-Aguilar FJ, Roman-Ramos R, Perez-Gutierrez S, Aguilar-Contreras A, Contreras-Weber CC, Flores-Saenz JL. Study of the anti-hyperglycemic effect of plants used as antidiabetics. J Ethnopharmacol. 1998; 61: 101–10.
4. Ali MS, Azhar I, Ahmad F, Ahmad AU, Smanghani KU. Antimicrobial screening of Mimosaceous plants. Pharm Biol. 2001; 39(1): 43-46.
5. Ananthan R, Latha M, Ramkumar K, Pari L, Baskar C, Bai V. Effect of Gymnema montanum leaves on serum and tissue lipids in alloxan diabetic rats. Exp Diabetes Res. 2003; 4: 183-89.
6. Banarjee, A. Studies on alkylated resin from Pithecellobium dulce. J Indian Chem Soc. 2005; 82: 186-87.
7. Baquer NZ, Gupta D, Raju J. Regulation of metabolic pathways in liver and kidney during experimental diabetes: effects of antidiabetic compounds. Indian J Clin Bioche. 1998; 13(2): 63–80.
8. Barrera NL, Bautista BS, Bravo LL, Bermudez TK, Garcia SF, Jimenez EM, Reyes CR. Antifungal activity against postharvest fungi by extracts and compounds of Pithecellobium dulce seeds (huamuchil). Acta Horticulturae. 2003; 628(2): 761-66.
9. Bautista BS, Garcia DE, Barrera NL, Reys CR, Wilson CL. Seasonal evaluation of the postharvest fungicidal activity of powders and extracts of huamuchil (Pithecellobium dulce): Action against Botrytis cinerea, Penicillium digitatum and Rhizopus stolonifer of straw berry fruit. Postharvest Biol Technol. 2003; 29: 81-92.
10. Bhargva Krishna, P., Gupta, M.B. and Chittranjan, R. Anti inflammatory activity of Saponins and other Natural Products. Indian J Med Res. 1970; 58: 724-30.
11. Bopanna KN, Kannan J, Sushma G, Balaraman R. Antidiabetic and antihyperlipidemic effects of neem seed kernel powder on alloxan diabetic rabbits. Indian J Pharmacol. 1997; 29: 162–67.
12. Delgado VF, Lopez VHE, Valdes RS, ¬Labra B, Chagolla LA, Lopez VEJ. Isolation and properties of a Kunitz-type protein inhibitor obtained from Pithecellobium dulce seeds. J Agri Food Chem. 2004; 52(20): 6115-21.
13. Devi M, Latha P. Antidiabetic effect of methanolic extract of Decalepis hamiltonii root (Wight and Arn) in normal and alloxan induced diabetic rats. J Pharm Res. 2013; 6: 166-72.
14. Felig P, Wahren J, Sherwin R, Palaiologos G. Amino acid and protein metabolism in diabetes mellitus. Arch Int Med. 1977; 137(4): 507-13.
15. Foster LB, Dunn RT. Stable reagents for determination of serum triglycerides by colorimetric Hantzsch condensation method. Clin Chem. 1973; 18: 338-40.
16. Gancedo JM, Gancedo C. Fructose-1,6-bisphosphatase, phosphofructo kinase and glucose-6-phophate dehydrogenase from fermenting and non-fermenting yeast. Arch Microbiol. 1971; 76: 132-38.
17. Gannon MC, Nuttall FQ. Effect of feeding, fasting, and diabetes on liver glycogen synthase activity, protein, and mRNA in rats. Diabetologia. 1997; 40: 758-63.
18. Gupta BL, Nehal M, Baquer NZ. Effect of experimental diabetes on the activities of hexokinase, glucose-6-phosphate dehydrogenase and catecholamines in rat erythrocytes of different ages. Indian J Exp Biol. 1997; 35: 792–95.
19. Henry RR. Thiazolidinedione. Endocrinol Metab Clin North Am. 1997; 26: 553-73.
20. Hultcrantz R, Glaumann H, Lindberg G, Nilsson LH. Liver investigation in 149 asymptomatic patients with moderately elevated activities of serum aminotransferase. Scand J Gastroenterol. 1986; 21(1): 109-13.
21. Ikino H, Kobayashi M, Suzuki Y, Konno C. Mechanism of hypoglycemic activity of aconitan A, a glycan from Aconitum carmichaelii roots. J Ethnopharmacol. 1989: 25: 295–304.
22. Jiang ZY, Woollard ACS, Wolff SP. Hydrogen peroxide production during experimental protein glycation. FEBS Lett. 1990; 268(1): 69–71.
23. Kaleem M, Asif M, Ahmed Q, Bano B. Antidiabetic and Antioxidant activity of Annona squamosa extract in streptozotocin induced diabetic rats. Singapore Med J. 2006; 47: 670-75.
24. Kameswara Rao B, Kesavulu MM, Appa Rao C. Evaluation of antidiabetic effect of Momordica cymbalaria hook fruit in alloxan diabetic rats. Fitoterapia, 2003; 74: 7-13.
25. Khan IA, Clark AM, McChesney JD. Antifungal activity of a new triterpenoid glycoside from Pithecellobium racemosum (M.). Pharm Res. 1997; 14(3): 358-61.
26. Kim MJ, Yoo KH, Kim JH, Seo YT, Ha BW, Kho JH, Shin YG, Chung CH. Effect of pinitol on glucose metabolism and adipocytokines in uncontrolled type 2 diabetes. Diabetes Res Clin Pract. 2007; 77(Suppl 1): S247-51.
27. King EJ, Armstrong AR. Determination of serum and bile phosphatase activity. Canad Med Assoc J. 1934; 31: 56-63.
28. King J. Practical Clinical Enzymology. D van Nostrand Co, Philadelphia, London. 1965; p. 1-301.
29. Kumar PA, Haseeb A, Suryanarayana P. Elevated expression of a A- and a-B crystallins in streptozotocin-induced diabetic rat. Arch Biochem Biophys. 2005; 444: 77–83.
30. Kumar S, Boulton AJ, Beck-Nielsen H, Berthezene F, Muggeo M, Persson B. Troglitazone Study Group, Troglitazone, an insulin action enhancer, improves metabolic control in NIDDM patients. Diabetologia. 1996; 39: 701-09.
31. Larcan A, Lambert H, Laprevote-Heully MC, Delorme N. Light and electron microscopic study of hepatic lesions in the course of hyperlactatemia in diabetic patients. Diabete Metab. 1979; 5(2): 103-12.
32. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin’s Phenol reagent. J Biol Chem. 1951; 193: 265-75.
33. Megala J, Geetha A. Free radical scavenging and H+,K+-ATPase inhibition activities of P. dulce. Food Chem. 2010; 121: 1120-28.
34. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase, J Biol Chem. 1972; 247(10): 3170–75.
35. Mohamed AK, Bierhaus A, Schiekofer S, Tritschler H, Ziegler R, Nawroth PP. The role of oxidative stress and NF-ðœ…B activation in late diabetic complications. Biofactors, 1999; 10(2-3): 157–67.
36. Morales MA, Jobbagy A, Terenzi HF. Mutations affecting accumulation of glycogen in Neurospora crassa. News Lett., 1973; 20: 24-25.
37. Moron MS, Depierre JN, Mannervik B. Levels of glutathione, glutathione reductase and glutathione-S-transferase activity in rat lung and liver. Biochim Biophys Acta. 1979; 582: 67–78.
38. Mule VS, Potdar VH, Jadhav SD, Disouza J. Neuropharmacological profile of aqueous and ethanolic extracts of P. dulce leaves in mice. Res J Pharm Pharmacodyn. 2011; 3(1): 27-30.
39. Murray RK, Granner DK, Mayes PA, Rodwell VW. Harper’s Biochemistry (25th ed.). Stanford, Connecticut: Appleton and Lange, 2000, pp. 610–17.
40. Nayak SS, Pattabiraman TN. A new colorimetric method for the estimation of glycosylated haemoglobin. Clin Chim Acta. 1981; 109: 267-74.
41. Osadebe PO, Omeje EO, Uzor PF, David EK, Obiorah DC. Seasonal variation for the antidiabetic activity of Loranthus micranthus methanol extract. Asian Pac J Trop Med. 2010; 3(3): 196–99.
42. Pal PB, Pal S, Manna P, Sil PC. Traditional extract of Pithecellobium dulce fruits protects mice against CCl4 induced renal oxidative impairment and necrotic cell death. Pathophysiol. 2012; 19: 101-14.
43. Palsamy P, Subramanian S. Resveratrol, a natural phytoalexin, normalizes hyperglycemia in streptozotocin-nicotinamide induced experimental diabetic rats. Biomed Pharmacother. 2008; 62: 598–605.
44. Parekh AC, Jung DH. Cholesterol determination with ferric acetate-uranium acetate and sulfuric acid-ferrous sulfate reagents. Anal Chem. 1970; 42: 1243-47.
45. Pithayanukul R, Ruenraroengsak P, Bavovada R, Pakmanee N, Suttisri R, Saenoon S. Inhibition of Naja kaouthia venom activities by plant polyphenols. J. Ethanopharmacol. 2005; 97(3): 527-33.
46. Pushparaj P, Tan CH, Tan BKH. Effects of Averrhoa bilimli leaf extract on blood glucose and lipids in streptozotocin diabetic rats. J Ethnopharmacol. 2000; 72: 69–76.
47. Rao GN, Nagender A, Sathyanarayana A, Rao G. Preparation, chemical composition and storage studies of qumachil P. dulce aril powder. J Food Sci Technol. 2011; 48(1) 90-95.
48. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalo acetic acid, glutamic pyruvate transaminase. Am J Clin Pathol. 1957; 28: 56-63.
49. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical role as a component of glatathione peroxidase, Science. 1973; 179(4073): 588–90.
50. Saravanan G, Ponmurugan P, Senthil Kumar GP, Rajarajan T. Modulatory effect of S-allylcysteine on glucose metabolism in streptozotocin induced diabetes rats. J Func. Foods. 2009; 1: 336-40.
51. Sedlak J, Lindsay RH. Estimation of the total, protein bound, and nonprotein sulfhydryl groups in tissue with Ellmans reagent. Anal Biochem. 1968; 25: 192-205.
52. Shanmugakumaran SD, Amerjothy S, Balakrishna K. Pharmacognostical, Antibacterial and antifungal potentials of the leaf extracts of Pithecellobium dulce Benth. Phcog Mag. 2006; 72: 163-67.
53. Sharma S, Choudhary M, Bhardwaj S, Choudhary N, Rana AC. Hypoglycemic potential of alcoholic root extract of Cassia occidentalis Linn in streptozotocin induced diabetes in albino mice. Bull Fac Pharm Cairo Univ. 2014; 52: 211-17.
54. Singh M, Khatoon S, Singh S, Kumar V, Rawaat AKS, Mehrotra S. Antimicrobial screening of ethnobotanically important stem bark of medicinal plants. Pharmacog Res. 2010; 2(4): 254-57.
55. Sugumaran M, Vetrichelvan T, Darline Quine S. Free radical scavenging activity of folklore: P. dulce Benth leaves. Ethnobot Leaflets, 2008a; 12: 446-51.
56. Sugumaran M, Vetrichelvan T, Darline Quine S. Locomotor activity of leaf extracts of P. dulce Benth. Ethnobot Leaflets. 2008b; 12: 490-93.
57. Sugumaran M, Vetrichelvan T, Darline Quine, S. Anti-diabetic potential of aqueous and alcoholic leaf extracts of Pithecellobium dulce. Asian J Research Chem. 2009; 2(1): 83-85.
58. Sukantha TA, Subashini KS, Ravindran NT, Balashanmugam P. Evaluation of in vitro antioxidant and antibacterial activity of Pithecellobium dulce Benth fruit peel. Int J Curr Res. 2011; 3(11): 378-82.
59. Sukantha TA, Subashini KS, Ravindran NT. Antibacterial activity of selected medicinal plant in traditional treatment of wound infection in Southeast India. Int J Pharm Pharm Sci. 2014; 6(11): 511-13.
60. Sundarrajan T, Rajkumar T, Udhayakumar E, Arunachalam G. Hypolipidemic activity of Pithecellobium dulce in Triton Wr-1339 induced hyperlipidemic activity in rats. Int J Chem Pharm Sci. 2010; 1(2): 50-53.
61. Takahara S, Hamilton HB, Nell JV. Hypocatalasemia: a new genetic carrier state, J Clin Invest. 1960; 39: 610–619.
62. Trinder P. Determination of blood glucose using an oxidaseperoxidase system with a non-carcinogenic chromogen. J Clin Pathol. 1969; 22: 158–61.
63. Venkateswaran S, Pari L. Effect of Coccinia indica on blood glucose, insulin and hepatic key enzymes in experimental diabetes. Pharmaceut. Biol. 2002; 40: 165–70.
64. Wolff SP, Dean RT. Glucose autoxidation and protein modification. The potential role of autoxidative glycosylation in diabetes. Biochem J. 1987; 245(1): 243–50.
Downloads
Published
2016-08-01
How to Cite
Sukantha, T., Sripathi, S. K., & Ravindran, N. T. (2016). Anti-Diabetic Activity Of Aqueous Extract Of Pithecellobium Dulce Benth Fruit Peel On Streptozotocin Induced Diabetic Rats. JOURNAL OF ADVANCES IN CHEMISTRY, 12(15), 4807–4815. https://doi.org/10.24297/jac.v12i15.1920
Issue
Section
Articles
License
All articles published in Journal of Advances in Linguistics are licensed under a Creative Commons Attribution 4.0 International License.
