Effect of Micronutrients on The Two Qatari Date Palm Cultivars Shishi and Lulu Through in Vitro Technic


  • Sara Aqeel Ministry of Municipality and Environment
  • Rehab A. Sidky Ministry of Municipality and Environment




In Vitro, Elongation Stage, Paclobutrazol, Copper Sulphate, Iron, Date Palm, Cultivars, Biodiversity


Plant tissue responds to nutrient media due to the plant cultivars genetic diversity. The objective of this research is to determine the effects of micronutrients on date palm growth and which of these micronutrients are critical for improving growth combined with Paclobutrazol or without on in vitro of elongation stage. In vitro growth of two cultivars were determined by varying five treatments that included minor salts (EDTA-chelated iron, CuSo4,) alone or combined with Paclobutrazol. The effects of these five treatments on plantlet length, trunk thickness, rooting number and Chlorophyll A, B was investigated. The results obtained after three months showed that, increasing copper sulphate on MS elongation media combined with Paclobutrazol was more effective for increasing the trunk thickness as well as rooting number compared with normal MS level. While poor response was found when Fe was increased in the chelated form of Fe-EDTA on MS elongation media.  For cultivars, Shishi gave the highest plantlets length as well as root number. The maximum content of chlorophylls A and B were observed when copper sulphate was increased on MS elongation media with cultivar Lulu. The survival rate after 3 months was related to the medium used during the elongation phase, the survival of plantlets reached to 98.66% with cultivar lulu at the acclimatization stage.


Download data is not yet available.

Author Biographies

Sara Aqeel, Ministry of Municipality and Environment

Agriculture Research Department, Ministry of Municipality and Environment. Qatar

Rehab A. Sidky, Ministry of Municipality and Environment

Agriculture Research Department, Ministry of Municipality and Environment. Qatar


Jaradat, A.A. 2011. Biodiversity of Date Palm. Cover and Soil Sciences, [Ed.-], in Encyclopedia of Life Support Systems (EOLSS), Developed under the Auspices of the UNESCO, Eolss Publishers, Oxford, UK.

Moursy, H.A. and M.M. Saker 1996. Date palm problems and need for biotechnology. Fifth International Conference on Desert Development, Texas Tech University, August 12-17, 1996, USA.

AlKhateeb, A. A. and S. M. Alturki 2014. A comparison of liquid and semi-solid cultures on shoot multiplication and rooting of three date palm cultivars (Phoenix dactylifera L.) in vitro. Adv. Environ. Biol., 8(16), 263-269.

Mazri, M. A. and R. Meziani 2015. Micropropagation of Date Palm: A Review. Cell Dev Biol 4: 160

Leifert, C., K.P. Murphy and P.J. Lumsden 1995. Mineral and carbohydrate nutrition of plant cell and tissue cultures. Crit Rev Plant Sci 14(2):83–109

Ramage, C.M and R.R. Williams 2002. Mineral nutrition and plant morphogenesis. In vitro Cell Dev Biol Plant 38:116–124

Marschner, H. 1995. Mineral nutrition of higher plants. Academic Press, New York, pp 1–5.

Williams, R.R. 1993. Mineral nutrition in vitro—a mechanistic approach. Aust J Bot 41:237–251

Murashige, T. and F. Skoog 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–49.

Hussein, K., K.K. Sahadevan and N. Salim 2010. Bioaccumulation and release of mercury in Vigna mungo (L.) hepper seedlings. J. Stress Phys. Biochem., 6: 56-63.

Dunlap, J.R. and K.M. Robacker 1988. Nutrient salts promote light-induced degradation of indole-3-acetic acid in tissue culture. Plant Physiology 88:379–382.

Mohamed, M.A.H. and A.A. Alsadon 2011. Effects of vessel type and growth regulators on micropropagation of Capsicum annuum. - Biol. Plant. 55: 370-374.

Gilley, A. and R.A. Fletcher 1997. Relative efficacy of paclobutrazol, propiconazole and tetraconazole as stress protectants in wheat seedlings. Plant Growth Regulation 21: 169-175.

Kamountsis, A.P. and A.G. Chronopoulou-Sereli 1999. Paclobutrazol affects growth and flower bud production in gardenia under different light regimes. Hort. Sci. 34-4: 674-675.

Fernandes, J.A., L. Balenzategui, S. Banon, J.A. Franco 2004. Induction of drought tolerance by paclobutrazol and irrigation deficit in Phillyrea angustifolia during the nursery period. - Sci. Hort. 107: 277-283.

Aqeel, S. and K. Elmeer 2011. Effects of Casein Hydrolysates and Glutamine on Callus and Somatic Embryogenesis of Date Palm (Phoenix dactylifera L.). New York Science Journal, 4: 121-125.

Costache, M.A., G.H. Câmpeanu, G. Nea?? 2012. Studies concerning the extraction of chlorophyll and total carotenoids from vegetables. Romanian Biotechnological Letters, 17: 7702-7708.

Sidky, R.A., M. AL-Salahi and A. Al-Mahmoud 2017. Establishment of an efficient protocol for rooting and acclimatization of two Qatari date palm cultivars Shishi and Lulu. International Journal Advanced Biological Research, 7: 1-5.

Steel, R.G., J.H. Torrie and D. Dickey 1997. Principles and Procedures of Statistics: A Biometrical Approach. The McGraw-Hill Co Inc, New York.

Reed, B.M., S. Wada, J. DeNoma, and R. P. Niedz 2013. Improving in vitro mineral nutrition for diverse pear germplasm. In vitro Cell.Dev. Biol 22.

Nas, M. N. 2004. The effects of elevated myo-inositol and copper on morphogenetic response of hazelnut (Corylus spp.) explants. KSU J. Sci. Engin., 7(1): 116-119.

Joshi, A. and S. L. Kothari 2007. High copper levels in the medium improves shoot bud differentiation and elongation from the cultured cotyledons of Capsicum annuum

Pra?ak R. and J. Molas 2015. Effect of copper concentration on micro-propagation and accumulation of some metals in the Dendrobium kingianum Bidwill Orchid. J. Elem.20(3): 693-703.

Rafail, S. T., H. D. Gharbia and N. Y. Atheel 2012. In vitro morphogenetic response of apple (Malusdomestica Borkh.) and pear (Pyrus communis L.) to the elevated levels of copper and myo-inositol. Acta Agrobot. 65(3): 43-48.

Dahleen, L.S. 1995. Improved plant regeneration from barley callus cultures by increased copper levels. Plant Cell Tiss. Org. Cult., 43: 267-269. DOI: 10.1007/BF00039954.

Sidky, R.A., Z.E. Zaid and A. El-Bana 2007. Optimized protocol for in vitro rooting of date palm (Phoenix dactylifera L.). Proc Fourth Int Symp on Date Palm, King Faisal University, Al Hassa, Saudi Arabia, pp 454–467.

Marino, G. 1988. The effect of paclobutrazol on in vitro rooting, transplant establishment and growth of fruit plants. Plant Growth Regulation 7: 237-247

Browning, G., A. Kuden and P. Blanke 1992. Site of (2RS, 3RS) Paclobutrazol promotion of axillary flower initiation in Pear cv. Doyenne du Comice. J. Hort. Sci. 67 (1), 121–128.

Ghosh, A., J. Chikara, D.R. Chaudhary, A.R. Prakash, G. Boricha and A. Zala 2010. Paclobutrazol arrests vegetative growth and unveils unexpressed yield potential of Jatropha curca. J. Plant Growth Regul. 29, 307–315.

Ouzounidou, G., E.P. Eleftheriou and S. Karataglis 1992. Ecological and ultrastructural effects ofcopper in Thlaspi ochroleucum (Cruciferae). Canad. J. Bot., 70: 947-957

Molassiotis, A.N., K.N. Dimassi, I.N. Therios and G.R. Diamantidis 2003. Fe-EDDHA promotes rooting of rootstock GF-677 (Prunus amygdalus P. persica) explants in vitro. Biol. Plant 47:141–144.

AL-Mayahi, A.M. 2014. Effect of copper sulphate and cobalt chloride on growth of the in vitro culture tissues for date palm (phoenix dactylifera l.) cv. Ashgar. American Journal of Agricultural and Biological Sciences 9 (1): 6-18.

Vinod, K., G. Awasthi and P.K. Chauhan 2012. Cu and Zn tolerance and responses of the Biochemical and Physiochemical system of Wheat. J. Stress Physiol

Purnhauser, L. and G. Gyulai 1993. Effect of copper on shoot and root regeneration in wheat, triticale, rape and tobacco tissue cultures. Plant Cell, Tiss. and Org. Cult. 35:131-139.




How to Cite

Aqeel, S., & A. Sidky, R. (2019). Effect of Micronutrients on The Two Qatari Date Palm Cultivars Shishi and Lulu Through in Vitro Technic. JOURNAL OF ADVANCES IN AGRICULTURE, 10, 1692–1704. https://doi.org/10.24297/jaa.v10i0.8252