Chlorophylla Fluorescence Imaging Technique for Fresh Quality Assessment of Tomato and Pepper FruitsStoredUnder Different Conditions
DOI:
https://doi.org/10.24297/jaa.v3i3.6570Keywords:
Tomato, Pepper, Chlorophyll fluorescence imaging, Fruit freshness, Chilling, Heat, Wet, StorageAbstract
The objective of this study was to find a rapid determination of the freshness of tomato (Solanum lycopersicum L.) and pepper (Capsicum annuum L.)fruits using portable chlorophyll fluorescence imaging instrument. To assess the fresh quality of tomato and pepper fruits, an imaging anlaysis of the photochemical responses of pericarp or exocarp of tomato and pepper fruits was performed with fruits preserved under the different storage conditions. The observed chlorophyll imaging photos were numerically transformed to the photochemical parameters on the basis of chlorophyll fluorescence.The storage conditions for fruits were regulated as follows; room temperature (control), heat (42°C), wet (25°C and 80% relative humidity), and chilling (4°C) conditions.
Chlorophyll fluorescence imaging (CFI) method showed that the decrease in Fv/Fmratios of pepper fruits was lower at room temperatureand under wet condition than the other conditions. Although Fv/Fmratios and ΦPSII values in tomato fruits showed low fluorescence responses, the changing patterns have permitted to determine th freshness. In heat condition, the photochemical parameters calculated from the images of Fv/Fm ratios, ΦPSII and non-photoquenching (NPQ) were also available to determine the freshness of fruits. In our study, it was clearly indicated that the chillingcondition was the suitable condition for thelong storage of tomato and pepper fruits. The CFI analysis is applicable as a rapid screening method for the determination of freshness of tomato and pepper fruits.
Downloads
References
Brodersen, K.P., Pedersen, O., Walker, I.R., Jensen, M.T., 2008. Respiration of midges (Diptera; Chironomidae) in British Columbian lakes: oxy?regulation, temperature and their role as palaeo?indicators. Freshwater Biology 53, 593-602.
Baker, N.R., 2008. Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu. Rev. Plant Biol. 59, 89-113.
Baker, N.R., Rosenqvist, E., 2004. Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. Journal of Experimental Botany 55, 1607-1621.
Strasser, R.J., Tsimilli-Michael, M., Srivastava, A., 2004. Analysis of the chlorophyll a fluorescence transient. Springer.
Yoo, S.Y., Ferrah, S., Kim, T.W., 2014. Chlorophyll fluorescence imaging analysis for fresh quality assessment of apple and kiwi fruits preserved under different storage conditions. International Journal of Advanced Information Science and Technology 29, 60-68.
Falkowski, P.G., Raven, J.A., 2013. Aquatic photosynthesis. Princeton University Press.
Ogweno, J.-O., Song, X.-S., Hu, W.-H., Shi, K., Zhou, Y.-H., Yu, J.-Q., 2009. Detached leaves of tomato differ in their photosynthetic physiological response to moderate high and low temperature stress. Scientia Horticulturae 123, 17-22.
Wahid, A., Gelani, S., Ashraf, M., Foolad, M.R., 2007. Heat tolerance in plants: an overview. Environmental and Experimental Botany 61, 199-223.
Chen, L.-S., Cheng, L., 2009. Photosystem 2 is more tolerant to high temperature in apple (Malus domestica Borkh.) leaves than in fruit peel. Photosynthetica 47, 112-120.
Chen, L.-S., Li, P., Cheng, L., 2009. Comparison of thermotolerance of sun-exposed peel and shaded peel of ‘Fuji’apple. Environmental and Experimental Botany 66, 110-116.
Chen, L.-S., Li, P., Cheng, L., 2008. Effects of high temperature coupled with high light on the balance between photooxidation and photoprotection in the sun-exposed peel of apple. Planta 228, 745-756.
Laval-Martin, D., Farineau, J., Diamond, J., 1977. Light versus dark carbon metabolism in cherry tomato fruits I. Occurrence of photosynthesis. Study of the intermediates. Plant Physiology 60, 872-876.
Rachmilevitch, S., DaCosta, M., Huang, B., 2006. Physiological and biochemical indicators for stress tolerance. Plant–environment interactions. 3rd ed. CRC Press, Boca Raton, FL, 321-356.
Nedbal, L., Whitmarsh, J., 2004. Chlorophyll fluorescence imaging of leaves and fruits, Chlorophyll a Fluorescence. Springer, pp 389-407.
Barbagallo, R.P., Oxborough, K., Pallett, K.E., Baker, N.R., 2003. Rapid, noninvasive screening for perturbations of metabolism and plant growth using chlorophyll fluorescence imaging. Plant Physiology 132, 485-493.
Genty, B., Briantais, J.-M., Baker, N.R., 1989. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta (BBA)-General Subjects 990, 87-92.
Genty, B., Wonders, J., Baker, N.R., 1990. Non-photochemical quenching of Fo in leaves is emission wavelength dependent: consequences for quenching analysis and its interpretation. Photosynthesis Research 26, 133-139.
Gorbe, E., Calatayud, A., 2012. Applications of chlorophyll fluorescence imaging technique in horticultural research: A review. Scientia Horticulturae 138, 24-35.
Lavi, N., Tadmor, Y., Meir, A., Bechar, A., Oren-Shamir, M., Ovadia, R., Reuveni, M., Nahon, S., Shlomo, H., Chen, L., Levin, I., 2009. Characterization of the intense pigment tomato genotype emphasizing targeted fruit metabolites and chloroplast biogenesis. J Agric Food Chem 57, 4818-4826.
Stadnichuk, I.N., Lukashev, E.P., Elanskaya, I.V., 2009. Fluorescence changes accompanying short-term light adaptations in photosystem I and photosystem II of the cyanobacterium Synechocystis sp. PCC 6803 and phycobiliprotein-impaired mutants: State 1/State 2 transitions and carotenoid-induced quenching of phycobilisomes. Photosynth Res 99, 227-241.
Goedheer, J.C., van der Tuin, A.K., 1967. Decline in bacteriochlorophyll fluorescence induced by carotenoid absorption. Biochim Biophys Acta 143, 399-407.
Kleinegris, D.M., van Es, M.A., Janssen, M., Brandenburg, W.A., Wijffels, R.H., 2010. Carotenoid fluorescence in Dunaliella salina. J Appl Phycol 22, 645-649.
Björkman, O., Demmig, B., 1987. Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins. Planta 170, 489-504.
Johnson, G., Young, A., Scholes, J., Horton, P., 1993. The dissipation of excess excitation energy in British plant species, Plant, Cell &Environment, 16, 673-679.
Fracheboud, Y., Haldimann, P., Leipner, J., Stamp, P., 1999. Chlorophyll fluorescence as a selection tool for cold tolerance of photosynthesis in maize (Zea mays L.). Journal of Experimental Botany 50, 1533-1540.
Ottander, C., Hundal, T., Andersson, B., Huner, N.P., Öquist, G., 1993. Photosystem II reaction centres stay intact during low temperature photoinhibition. Photosynthesis Research 35, 191-200.
Havaux, M., Strasser, R.J., Greppin, H., 1991. A theoretical and experimental analysis of the qP and qN coefficients of chlorophyll fluorescence quenching and their relation to photochemical and nonphotochemical events. Photosynthesis Research 27, 41-55.
Baker, N.R., 1991. A possible role for photosystem II in environmental perturbations of photosynthesis. Physiologia Plantarum 81, 563-570.
Downloads
Published
How to Cite
Issue
Section
License
All articles published in Journal of Advances in Linguistics are licensed under a Creative Commons Attribution 4.0 International License.
