Utility of Certain Nucleophilic Aromatic Substitution Reactions for the Assay of Ethamsylate in its Dosage forms and in Presence of its Degradation Product

Authors

  • F. Belal University of Mansoura , 35516, Mansoura
  • M. Sharaf EL-Din University of Mansoura, 35516, Mansoura
  • M. M. Tolba University of Mansoura , 35516, Mansoura
  • H. Alaa University of Mansoura , 35516, Mansoura

DOI:

https://doi.org/10.24297/jac.v7i1.974

Keywords:

Ethamsylate, hydroquinone, spectrophotometry, naphthoquinone, dinitrofluorobenzene

Abstract

The study represents the first report on the development of spectrophotometric methods for determination of ethamsylate (EST) in the presence of hydroquinone as an impurity and/or acidic degradation product. The proposed methods are based on the reaction of EST through it,s secondary amino group either with 1,2-naphthoquinone-4-sulphonate sodium (NQS) at pH 10.7 or 2,4-dinitrofluorobenzene (DNFB) at pH 9.3 to form orange and yellow colored reaction products peaking at 478 and 387 nm for methods (I) and (II), respectively. The different experimental parameters affecting the development and stability of the reaction products in methods (I) and (II) were carefully studied and optimized. The absorbance-concentration plots were rectilinear over the concentration ranges of 2-30 and 2-14 µg mL−1 for methods (I) and (II), respectively. The lower detection limits were 0.13 and 0.19 µg mL−1 and the lower quantitation limits were 0.44 and 0.63 µg mL−1 for methods (I) and (II), respectively. Both methods were successfully applied to commercial ampoules and tablets.

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Author Biographies

F. Belal, University of Mansoura , 35516, Mansoura

Department of Analytical Chemistry, Faculty of Pharmacy

M. Sharaf EL-Din, University of Mansoura, 35516, Mansoura

Department of Analytical Chemistry, Faculty of Pharmacy

M. M. Tolba, University of Mansoura , 35516, Mansoura

Department of Analytical Chemistry, Faculty of Pharmacy

H. Alaa, University of Mansoura , 35516, Mansoura

Department of Analytical Chemistry, Faculty of Pharmacy

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Published

2011-04-19

How to Cite

Belal, F., EL-Din, M. S., Tolba, M. M., & Alaa, H. (2011). Utility of Certain Nucleophilic Aromatic Substitution Reactions for the Assay of Ethamsylate in its Dosage forms and in Presence of its Degradation Product. JOURNAL OF ADVANCES IN CHEMISTRY, 7(1), 1223–1239. https://doi.org/10.24297/jac.v7i1.974

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