Cell Imaging with Fluorescent Bi-Metallic Nanoparticles
DOI:
https://doi.org/10.24297/jac.v11i4.6694Keywords:
Nanoparticles of noble metals· Fluorescence ·Toxicity of nanoparticles· Yeast Hansenula polymorpha ·Cell imaging .Abstract
Last decades various imaging techniques have been applied in biological and biomedical research, such as magnetic resonance imaging, different types of tomography, fluorescence/bioluminescence, ultrasound, as well as multimodality approaches. Fluorescence imaging, especially in combination with nanoscale materials, is a very prospective tool for experiments in vivo and clinical applications due to its high temporal and spatial resolutions. Fluorescent nanoparticles (NPs), having ability to interact with biomolecules both on the surface of and inside the cells, may revolutionize the cell imaging approaches for diagnostics and therapy. In our investigation we report about new method of cell imaging with fluorescent bi-metallic NPs synthesized by chemical reduction of the relevant ions. As the model of living organism, the cells of yeast Hansenula polymorpha were used. All NPs in minimal concentration (up to 0.05 mM) was proved to be non-toxic for yeast cells. The NPs and NPs-modified cells were characterized with the methods of UV-VIS spectroscopy, scanning electron microscopy, atom force microscopy, transmission electron microscopy and fluorescence microscopy. The bimetallic NPs, possessing the stable fluorescence in solution and inside the cells, allow to observe the phenomenon of NPs transferring from parental to daughter cells through at least three generations followed by releasing from the modified cells. The fluorescent NPs synthesized being small, non-toxic and fluorescent was shown to be perspective tool for cell imaging.
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References
[1] Key, J., Leary J.F. 2014. Nanoparticles for multimodal in vivo imaging in nanomedicine. Int. J. Nanomedicine. 9, 711-726.
[2] Boisselier, E., Astruc, D. 2009. Gold nanoparticles in nanomedicine: preparations, imaging, diagnostics, therapies and toxicity. Chem. Soc. Rev. 38, 1759–1782.
[3] Bao, G., Mitragotri, S., Tong, S. 2013. Multifunctional nanoparticles for drug delivery and molecular imaging. Annu Rev. Biomed. En. 15, 253-282.
[4] Kreuter, J. 2014. Nanoparticulate systems for brain delivery of drugs. Adv. Drug Deliv. Rev. 71, 2–14.
[5] Swierczewska, M., Lee, S., Chen, X. 2011. Inorganic nanoparticles for multimodal molecular imaging. Mol. Imaging. 10(1), 3-16.
[6] Zhu, Y., Hong, H., Xu, Z.P., Li, Z., Cai, W. 2013. Quantum dot-based nanoprobes for in vivo targeted imaging. Curr. Mol. Med. 13(10), 1549-1567.
[7] Gowda, R., Jones, N.R., Banerjee, S., Robertson, G.P. 2013. Use of nanotechnology to develop multi-drug ihibitors for cancer therapy. J. Nanomed. Nanotechnol. 4(6): doi:10.4172/2157-7439.1000184
[8] Guo, S., Wang, E. 2011. Noble metal nanomaterials: controllable synthesis and application in fuel cells and analytical sensors. Nano Today. 6, 240-264.
[9] You, H., Yang, S., Ding, B., Yang, H. 2013. Synthesis of colloidal metal and metal alloy nanoparticles for electrochemical energy applications. Chem. Soc.
Rev. 42, 2880-2904.
[10] Mahmood Aliofkhazraei. 2014. Modern electrochemical methods in nano, surface and corrosion science. ISBN 978-953-51-1586-1, 350 p, InTech, June 11, 2014 doi: 10.5772/57202.
[11] Garcia-Torres, J., Vallés, E., Gómez, E. 2010. Synthesis and characterization of Co@Ag core-shell nanoparticles. J. Nanopart. Res. 12, 2189–2199.
[12] Xue, C., Millstone, J.E., Li, S., Mirkin, C.A. 2007. Plasmon-driven synthesis of triangular core-shell nanoprisms from gold seeds. Angew. Chem. Int. Ed. 46, 8436–8439.
[13] Blosi, M., Albonetti, S., Ortelli, S., Costa, L. 2014. Green and easily scalable microwave synthesis of noble metal nanosols (Au, Ag, Cu, Pd) usable as catalysts. New J. Chem. 38, 1401-1409.
[14] Zhao, J., Hu, W., Li, H., Ji, M., Zhao, C., Wanq, Z., Hu, H. 2015. One-step green synthesis of a ruthenium/graphene composite as a highly efficient catalyst. RSC Adv. 5, 7679-7686.
[15] Mittal, A.K., Chisti, Y., Banerjee. U.C. 2013. Synthesis of metallic nanoparticles using plant extracts. Biotechnology Advances. 31(2), 346-356.
[16] Nadagouda, M. N. 2012. Green synthesis of nanocrystals and nanocomposites. In: Kolesnikov N (ed) Modern aspects of bulk crystal and thin film preparation. InTech http://www.intechopen.com/books/modern-aspects-of-bulk-crystal-and-thin-film-preparation/green-synthesis-of-nanocrystals-and-nanocomposites.
[17] Bohren, C., Huffman, D. 1983. Absorption and scattering of light by small particles. Wiley, New York.
[18] Zhu, J., Wang, Y., Huang, L., Lu, Y. 2004. Resonance light scattering characters of core-shell structure of Au-Ag nanoparticles. Phys. Lett. A. 323, 455–459.
[19] Mulvaney, P., Giersig, M., Henglein, A. 1993. Electrochemistry of multilayer colloids: preparation and absorption spectrum of gold-coated silver particles. J Phys. Chem. 97, 7061–7064.
[20] Lee, A., Baddeley, C., Hardacre, C., Ormerod, R.M., Lambert, R.M., Schmid, G., West, H. 1995. Structural and catalytic properties of novel Au/Pd bimetallic colloid particles: EXAFS, XRD, and acetylene coupling. J. Phys. Chem. 99, 6096–6102.
[21] Knauer, A., Thete, A., Li, S., Romanus, H., Csáki, A., Fritzsche, W., Köhler, J. M. 2011. Au/Ag/Au double shell nanoparticles with narrow size distribution obtained by continuous micro segmented flow synthesis. Chem Eng J 166:1164–1171.
[22] Yang, Y., Shi, J.L., Kawamura, G., Nogami. M. 2008. Preparation of Au-Ag, Ag-Au core-shell bimetallic nanoparticles for surface-enhanced Raman scattering. Scripta Mater. 58, 862–865.
[23] Rodriguez-Gonzalez, B., Burrows, A., Watanabe, M., Kiely, C.J., Liz Marzan, L.M. 2005. Multishell bimetallic AuAg nanoparticles: synthesis, structure and optical properties. J. Mater. Chem. 15, 1755–1759.
[24] Cao, Y.W., Jin, R., Mirkin, C.A. 2001. DNA-modified core-shell Ag/Au nanoparticles. J. Am. Chem. Soc. 123, 7961–7962.
[25] Rivas, L., Sanchez-Cortes, S., Garcia-Ramos, J.V., Morcillo, G. 2000. Mixed silver/gold colloids: a study of their formation, morphology, and surface-enhanced Raman activity. Langmuir. 16, 9722–9728.
[26] Mohamed, M.B., Volkov, V., Link, S., El-Sayed, M.A. 2000. The lighting gold nanorods: fluorescence enhancement of over a million compared to the gold metal. Chem. Phys. Lett. 317, 517–523.
[27] Wilcoxon, J. P.; Martin, J. E.; Parsapour, F.; Wiedenman, B.; Kelley, D. F. 1998. Photoluminescence from Nanosize Gold Clusters. J. Chem. Phys. 108, 9137–9143. [28] Jian, Z., Xiang, Z., Yongchang, W. 2005. Electrochemical synthesis and fluorescence spectrum properties of silver nanospheres. Microelectron. Eng. 77, 58–62. [29] Mooradian, A. 1969. Photoluminescence of metals. Phys Rev Lett 22, 185–187.
[30] Liu, M., Guyot-Sionnest, P. 2004. Synthesis and optical characterization of Au/Ag core/shell nanorods. J. Phys. Chem. B. 108, 5882–5888.
[31] Zhang, L., Wang, E. 2014. Metal nanoclusters: new fluorescent probes for sensors and bioimaging. Nano Today. 9, 132–157.
[32] Bamrungsap, S., Zhao, Z., Chen, T., Wang, L., Li, C., Fu, T., Tan, W. 2012. Nanotechnology in therapeutics: a focus on nanoparticles as a drug delivery system. Nanomedicine. 7, 1253–1271.
[33] Connor, E., Mwamuka, J., Gole, A., Murphy, C., Wyatt, M. 2005. Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity. Small. 1, 325–327.
[34] Sezer, A.D. Application of nanotechnology in drug delivery. ISBN 978-953-51-1628-8, 552 p. InTech, Chapters published July 25, 2014. DOI:10.5772/57028.
[35] Akhter, S., Ahmad, M.Z., Ahmad, F.J., Storm, G., Kok, R.J. 2012. Gold nanoparticles in theranostic oncology: current state-of-the-art. Expert. Opin. Drug Deliv. 9, 1225–1243.
[36] Lynch, I., Weiss, C., Valsami-Jones, E. 2014. A strategy for grouping of nanomaterials based on key physico-chemical descriptors as a basis for safer-by-design NMs. Nano Today. 9, 266–270.
[37] Lemire, J.A., Harrison, J.J., Turner, R.J. 2013. Antimicrobial activity of metals: Mechanisms, molecular targets and applications. Nature reviews/Microbiology.
11, 371–384.
[38] Kuhn, D.A., Vanhecke, D., Michen, B., Blank, F., Gehr, P., Petri-Fink, A., Rothen-Rutishauser, B. 2014. Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages. Beilstein J. Nanotechnol. 5, 1625–1636.
[39] Mironava, T., Hadjiargyrou, M., Simon. M., Jurukovski, V., Rafailovich, M.H. 2010. Gold nanoparticles cellular toxicity and recovery: Effect of size, concentration and exposure time. Nanotoxicology. 4, 120–137.
[40] Hoet, P.H., Nemery, B., Napierska, D. 2013. Intracellular oxidative stress caused by nanoparticles: What do we measure with the dichlorofluorescein assay? Nano Today. 8, 223–227.
[41] Wang, L., Clavero, C., Huba, Z., Carroll, E. E., Carpenter, D. Gu, Lukaszew, R. A. 2011. Plasmonics and enhanced magneto-optics in core-shell Co-Ag nanoparticles. Nano Lett 11:1237–1240
[42] Stasyuk, N., Serkiz, R., Mudry, S., Gayda, G., Zakalskiy, A., Koval'chuk, Y., Gonchar, M. 2011. Recombinant human arginase I immobilized on gold and silver nanoparticles: preparation and properties. Nanotechnology Development 1, 11-15
[43] Synenka, M.M., Stasyuk, N.Ye., Semashko, T.V., Gayda, G.Z., Mikchailova, R.V., Gonchar M.V. 2014. Immobilization of oxidoreductases at gold and silver nanoparticles. Studia Biologica 8:5–16 (in Ukrainian).
[44] Stasyuk, N., Gayda, G., Gonchar, M. 2014. L-Arginine-selective microbial amperometric sensor based on recombinant yeast cells over-producing human liver arginase I. Sens Actuators B. 204, 515–521.
[45] Harriman, A. 1990. Bimetallic Pt–Au colloids as catalysts for photochemical dehydrogenation. J. Chem. Soc. Chem. Commun. 1, 24-26.
[46] Mallik, K., Mandal, M., Pradhan, N., Pal, T. 2001. Seed mediated formation of bimetallic nanoparticles by UV Irradiation: a photochemical approach for the preparation of “core-shell†type structures. Nano Lett. 1, 319–322
[47] Vodnik, V.V., Bozanic, D.K., Bibic, N., Šaponjić, Z.V., Nedeljković, J.M. 2008. Optical properties of shaped silver nanoparticles. J. Nanosci. Nanotechnol. 8,3511-3515. [48] Cao-Milán, R., Liz-Marzán, L.M. 2014. Gold nanoparticle conjugates: recent advances toward clinical applications. Expert Opin. Drug Deliv. 11, 741-752
[2] Boisselier, E., Astruc, D. 2009. Gold nanoparticles in nanomedicine: preparations, imaging, diagnostics, therapies and toxicity. Chem. Soc. Rev. 38, 1759–1782.
[3] Bao, G., Mitragotri, S., Tong, S. 2013. Multifunctional nanoparticles for drug delivery and molecular imaging. Annu Rev. Biomed. En. 15, 253-282.
[4] Kreuter, J. 2014. Nanoparticulate systems for brain delivery of drugs. Adv. Drug Deliv. Rev. 71, 2–14.
[5] Swierczewska, M., Lee, S., Chen, X. 2011. Inorganic nanoparticles for multimodal molecular imaging. Mol. Imaging. 10(1), 3-16.
[6] Zhu, Y., Hong, H., Xu, Z.P., Li, Z., Cai, W. 2013. Quantum dot-based nanoprobes for in vivo targeted imaging. Curr. Mol. Med. 13(10), 1549-1567.
[7] Gowda, R., Jones, N.R., Banerjee, S., Robertson, G.P. 2013. Use of nanotechnology to develop multi-drug ihibitors for cancer therapy. J. Nanomed. Nanotechnol. 4(6): doi:10.4172/2157-7439.1000184
[8] Guo, S., Wang, E. 2011. Noble metal nanomaterials: controllable synthesis and application in fuel cells and analytical sensors. Nano Today. 6, 240-264.
[9] You, H., Yang, S., Ding, B., Yang, H. 2013. Synthesis of colloidal metal and metal alloy nanoparticles for electrochemical energy applications. Chem. Soc.
Rev. 42, 2880-2904.
[10] Mahmood Aliofkhazraei. 2014. Modern electrochemical methods in nano, surface and corrosion science. ISBN 978-953-51-1586-1, 350 p, InTech, June 11, 2014 doi: 10.5772/57202.
[11] Garcia-Torres, J., Vallés, E., Gómez, E. 2010. Synthesis and characterization of Co@Ag core-shell nanoparticles. J. Nanopart. Res. 12, 2189–2199.
[12] Xue, C., Millstone, J.E., Li, S., Mirkin, C.A. 2007. Plasmon-driven synthesis of triangular core-shell nanoprisms from gold seeds. Angew. Chem. Int. Ed. 46, 8436–8439.
[13] Blosi, M., Albonetti, S., Ortelli, S., Costa, L. 2014. Green and easily scalable microwave synthesis of noble metal nanosols (Au, Ag, Cu, Pd) usable as catalysts. New J. Chem. 38, 1401-1409.
[14] Zhao, J., Hu, W., Li, H., Ji, M., Zhao, C., Wanq, Z., Hu, H. 2015. One-step green synthesis of a ruthenium/graphene composite as a highly efficient catalyst. RSC Adv. 5, 7679-7686.
[15] Mittal, A.K., Chisti, Y., Banerjee. U.C. 2013. Synthesis of metallic nanoparticles using plant extracts. Biotechnology Advances. 31(2), 346-356.
[16] Nadagouda, M. N. 2012. Green synthesis of nanocrystals and nanocomposites. In: Kolesnikov N (ed) Modern aspects of bulk crystal and thin film preparation. InTech http://www.intechopen.com/books/modern-aspects-of-bulk-crystal-and-thin-film-preparation/green-synthesis-of-nanocrystals-and-nanocomposites.
[17] Bohren, C., Huffman, D. 1983. Absorption and scattering of light by small particles. Wiley, New York.
[18] Zhu, J., Wang, Y., Huang, L., Lu, Y. 2004. Resonance light scattering characters of core-shell structure of Au-Ag nanoparticles. Phys. Lett. A. 323, 455–459.
[19] Mulvaney, P., Giersig, M., Henglein, A. 1993. Electrochemistry of multilayer colloids: preparation and absorption spectrum of gold-coated silver particles. J Phys. Chem. 97, 7061–7064.
[20] Lee, A., Baddeley, C., Hardacre, C., Ormerod, R.M., Lambert, R.M., Schmid, G., West, H. 1995. Structural and catalytic properties of novel Au/Pd bimetallic colloid particles: EXAFS, XRD, and acetylene coupling. J. Phys. Chem. 99, 6096–6102.
[21] Knauer, A., Thete, A., Li, S., Romanus, H., Csáki, A., Fritzsche, W., Köhler, J. M. 2011. Au/Ag/Au double shell nanoparticles with narrow size distribution obtained by continuous micro segmented flow synthesis. Chem Eng J 166:1164–1171.
[22] Yang, Y., Shi, J.L., Kawamura, G., Nogami. M. 2008. Preparation of Au-Ag, Ag-Au core-shell bimetallic nanoparticles for surface-enhanced Raman scattering. Scripta Mater. 58, 862–865.
[23] Rodriguez-Gonzalez, B., Burrows, A., Watanabe, M., Kiely, C.J., Liz Marzan, L.M. 2005. Multishell bimetallic AuAg nanoparticles: synthesis, structure and optical properties. J. Mater. Chem. 15, 1755–1759.
[24] Cao, Y.W., Jin, R., Mirkin, C.A. 2001. DNA-modified core-shell Ag/Au nanoparticles. J. Am. Chem. Soc. 123, 7961–7962.
[25] Rivas, L., Sanchez-Cortes, S., Garcia-Ramos, J.V., Morcillo, G. 2000. Mixed silver/gold colloids: a study of their formation, morphology, and surface-enhanced Raman activity. Langmuir. 16, 9722–9728.
[26] Mohamed, M.B., Volkov, V., Link, S., El-Sayed, M.A. 2000. The lighting gold nanorods: fluorescence enhancement of over a million compared to the gold metal. Chem. Phys. Lett. 317, 517–523.
[27] Wilcoxon, J. P.; Martin, J. E.; Parsapour, F.; Wiedenman, B.; Kelley, D. F. 1998. Photoluminescence from Nanosize Gold Clusters. J. Chem. Phys. 108, 9137–9143. [28] Jian, Z., Xiang, Z., Yongchang, W. 2005. Electrochemical synthesis and fluorescence spectrum properties of silver nanospheres. Microelectron. Eng. 77, 58–62. [29] Mooradian, A. 1969. Photoluminescence of metals. Phys Rev Lett 22, 185–187.
[30] Liu, M., Guyot-Sionnest, P. 2004. Synthesis and optical characterization of Au/Ag core/shell nanorods. J. Phys. Chem. B. 108, 5882–5888.
[31] Zhang, L., Wang, E. 2014. Metal nanoclusters: new fluorescent probes for sensors and bioimaging. Nano Today. 9, 132–157.
[32] Bamrungsap, S., Zhao, Z., Chen, T., Wang, L., Li, C., Fu, T., Tan, W. 2012. Nanotechnology in therapeutics: a focus on nanoparticles as a drug delivery system. Nanomedicine. 7, 1253–1271.
[33] Connor, E., Mwamuka, J., Gole, A., Murphy, C., Wyatt, M. 2005. Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity. Small. 1, 325–327.
[34] Sezer, A.D. Application of nanotechnology in drug delivery. ISBN 978-953-51-1628-8, 552 p. InTech, Chapters published July 25, 2014. DOI:10.5772/57028.
[35] Akhter, S., Ahmad, M.Z., Ahmad, F.J., Storm, G., Kok, R.J. 2012. Gold nanoparticles in theranostic oncology: current state-of-the-art. Expert. Opin. Drug Deliv. 9, 1225–1243.
[36] Lynch, I., Weiss, C., Valsami-Jones, E. 2014. A strategy for grouping of nanomaterials based on key physico-chemical descriptors as a basis for safer-by-design NMs. Nano Today. 9, 266–270.
[37] Lemire, J.A., Harrison, J.J., Turner, R.J. 2013. Antimicrobial activity of metals: Mechanisms, molecular targets and applications. Nature reviews/Microbiology.
11, 371–384.
[38] Kuhn, D.A., Vanhecke, D., Michen, B., Blank, F., Gehr, P., Petri-Fink, A., Rothen-Rutishauser, B. 2014. Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages. Beilstein J. Nanotechnol. 5, 1625–1636.
[39] Mironava, T., Hadjiargyrou, M., Simon. M., Jurukovski, V., Rafailovich, M.H. 2010. Gold nanoparticles cellular toxicity and recovery: Effect of size, concentration and exposure time. Nanotoxicology. 4, 120–137.
[40] Hoet, P.H., Nemery, B., Napierska, D. 2013. Intracellular oxidative stress caused by nanoparticles: What do we measure with the dichlorofluorescein assay? Nano Today. 8, 223–227.
[41] Wang, L., Clavero, C., Huba, Z., Carroll, E. E., Carpenter, D. Gu, Lukaszew, R. A. 2011. Plasmonics and enhanced magneto-optics in core-shell Co-Ag nanoparticles. Nano Lett 11:1237–1240
[42] Stasyuk, N., Serkiz, R., Mudry, S., Gayda, G., Zakalskiy, A., Koval'chuk, Y., Gonchar, M. 2011. Recombinant human arginase I immobilized on gold and silver nanoparticles: preparation and properties. Nanotechnology Development 1, 11-15
[43] Synenka, M.M., Stasyuk, N.Ye., Semashko, T.V., Gayda, G.Z., Mikchailova, R.V., Gonchar M.V. 2014. Immobilization of oxidoreductases at gold and silver nanoparticles. Studia Biologica 8:5–16 (in Ukrainian).
[44] Stasyuk, N., Gayda, G., Gonchar, M. 2014. L-Arginine-selective microbial amperometric sensor based on recombinant yeast cells over-producing human liver arginase I. Sens Actuators B. 204, 515–521.
[45] Harriman, A. 1990. Bimetallic Pt–Au colloids as catalysts for photochemical dehydrogenation. J. Chem. Soc. Chem. Commun. 1, 24-26.
[46] Mallik, K., Mandal, M., Pradhan, N., Pal, T. 2001. Seed mediated formation of bimetallic nanoparticles by UV Irradiation: a photochemical approach for the preparation of “core-shell†type structures. Nano Lett. 1, 319–322
[47] Vodnik, V.V., Bozanic, D.K., Bibic, N., Šaponjić, Z.V., Nedeljković, J.M. 2008. Optical properties of shaped silver nanoparticles. J. Nanosci. Nanotechnol. 8,3511-3515. [48] Cao-Milán, R., Liz-Marzán, L.M. 2014. Gold nanoparticle conjugates: recent advances toward clinical applications. Expert Opin. Drug Deliv. 11, 741-752
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Published
2015-03-09
How to Cite
Stasyuk, N. Y., Gayda, G. Z., Serkiz, R. J., & Gonchar, M. V. (2015). Cell Imaging with Fluorescent Bi-Metallic Nanoparticles. JOURNAL OF ADVANCES IN CHEMISTRY, 11(4), 3499–3511. https://doi.org/10.24297/jac.v11i4.6694
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