Markers characterizing corneal damage during aging of rat

Authors

  • Hassan I. H. El-Sayyad Mansoura University, Mansoura 002050 2254850
  • Ahmed A. El-Mansi Mansoura Univ., Egypt
  • Mona S Guida Mansoura University, Mansoura
  • Ezaldin AM Mohammed Omar Al- Mokhtar University

DOI:

https://doi.org/10.24297/jac.v11i5.4471

Keywords:

Aging, cornea, immunohistochemistry, flow cytometry, transmission electron microscopy.

Abstract

Aging is a biological phenomenon that involves an increase of oxidative stress associated with gradual degradation of the structure and function of the cornea. Gender differences and subsequent deterioration of cornea is an interesting topic, especially yet few data are available concerning the impact of age, especially on the corneal. One hundred male and female Wistar albino rats ages 3, 6, 18, 24, and 30 months (n=10 equal for male and female) were used. At the time interval, cornea were investigated by light and transmission electron microscopy (TEM), immunohistochemistry of caspase 3 (casp3), glial fibrillar acidic protein(GFAP) and CD45 and  flow cytometry of DNA, bcl-2-like protein 4 (BAX), transforming growth factor beta (TGF-β) and Cd45 (lymphocyte common antigen). Light and TEM investigation  revealed apparent deterioration of atrophy of corneal epithelium with vesicular vacuolar degeneration, hyalinization of stromal collagen fibrils and swelling and degeneration of the endothelial lining the descemet's membrane. There was apparent loss of keratocytes within corneal stroma. Immunohistochemistry of casp 3 and CD45 were markedly increased manifesting cell damage. GFAP showed apparent reduction of innervation of corneal stroma and endothelium layer. Flow cytometry of DNA, Bax and TGF revealed increased apoptic cell death of cornea of 30M-old rats. We concluded that aging contributed to an apparent increase of cellular damage of different corneal region associated with alterations of cell markers.

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Author Biographies

Hassan I. H. El-Sayyad, Mansoura University, Mansoura 002050 2254850

Department of Zoology, Faculty of Science

Ahmed A. El-Mansi, Mansoura Univ., Egypt

Zoology Dept. Faculty of Science

Mona S Guida, Mansoura University, Mansoura

Department of Pediatrics, Faculty of Medicine

Ezaldin AM Mohammed, Omar Al- Mokhtar University

Department of Zoology , Faculty Science

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Published

2015-04-18

How to Cite

El-Sayyad, H. I. H., El-Mansi, A. A., Guida, M. S., & Mohammed, E. A. (2015). Markers characterizing corneal damage during aging of rat. JOURNAL OF ADVANCES IN CHEMISTRY, 11(5), 3532–3539. https://doi.org/10.24297/jac.v11i5.4471

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Articles