An HPLC Method for the Determination of Saxagliptin in Human Plasma with Fluorescence Detection

Authors

  • Serife Evrim Kepekci Tekkeli Bezmialem Vakif University
  • Mustafa Volkan K?z?lta? Bezmialem Vakif University, 34093, Fatih- Istanbul
  • Demet Dincel Bezmialem Vakif University, 34093, Fatih- Istanbul

DOI:

https://doi.org/10.24297/jac.v5i3.2678

Keywords:

Fluorescamine, HPLC, pharmacokinetic, pre-column derivatization, saxagliptin, plasma

Abstract

A sensitive and selective HPLC method with fluorometric detection was developed for the determination of saxagliptin (SGX) in human plasma and applied to a pharmacokinetic study. SGX was precolumn derivatized with fluorescamine, and the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 1.3 ml/min. The method was based on the measurement of the derivative using fluorescence detection at 378 nm, with excitation at 463 nm. The calibration curve was linear over the range of 3.0“100.0 ng/ml. LOD and LOQ were found to be 0.15 and 0.5 ng/ml, respectively. Intraday and interday RSD values were less than 2.84%. The plasma concentration“time profile and pharmacokinetic parameters such as AUC0–t, AUC0–∞, Cmax, tmax, t1/2, were calculated according to the assays.

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Author Biographies

Serife Evrim Kepekci Tekkeli, Bezmialem Vakif University

Faculty of Pharmacy, Department of Analytical Chemistry

Mustafa Volkan K?z?lta?, Bezmialem Vakif University, 34093, Fatih- Istanbul

Faculty of Pharmacy, Department of Analytical Chemistry

Demet Dincel, Bezmialem Vakif University, 34093, Fatih- Istanbul

Faculty of Pharmacy, Department of Analytical Chemistry

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Published

2009-12-02

How to Cite

Kepekci Tekkeli, S. E., K?z?lta?, M. V., & Dincel, D. (2009). An HPLC Method for the Determination of Saxagliptin in Human Plasma with Fluorescence Detection. JOURNAL OF ADVANCES IN CHEMISTRY, 5(3), 810–818. https://doi.org/10.24297/jac.v5i3.2678

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