ACE gene polymorphism and serum ACE level with Progression of Nephropathy in Type 2 Diabetic Patients

Authors

  • Olfat Gamil Shaker Cairo University
  • Manal Fouad Ismail Cairo University
  • Esmat Ashour National Research Centre, Dokki, Giza, Egypt
  • Heba Mourad Yousif Cairo University
  • Mie Afify National Research Centre, Dokki, Giza
  • Weaam Gouda National Research Centre, Dokki, Giza

DOI:

https://doi.org/10.24297/jac.v9i3.1009

Keywords:

Diabetic nephropathy(DN), Type 2 diabetes mellitus (T2DM), Angiotensin-converting enzyme(ACE), Gene polymorphism, Serum ACE levels

Abstract

Background. One of the most common complications of diabetes mellitus (DM) is diabetic nephropathy (DN).  Angiotensin- converting enzyme (ACE) gene was the first candidate gene of renin-angiotensin system (RAS) for predisposition to DN.

Objective. Investigation whether the ACE insertion/deletion (I/D) polymorphism is associated with Egyptian type 2 diabetic patients (T2DM) with nephropathy. In addition, the study investigated the relationship between variants of ACE I/D gene polymorphism and serum ACE level and the progression of nephropathy in Egyptian T2DM patients.

Methods.  A total of 85 T2DM patients (45 with nephropathy and 40 without nephropathy) besides 45 healthy (non-diabetic) age-matched subjects were recruited in this study for comparison. The (I/D) polymorphism of the ACE gene was investigated using PCR and serum ACE levels were determined using ELISA.

Results. The frequency of ACE DD genotype and D allele was significantly higher in DN patients when compared to control healthy subjects and diabetic patients without nephropathy. In addition our results showed a significant association between DD genotype of ACE gene and elevated serum ACE level.

Conclusion. The present study showing a strong association between the D allele and/or DD homozygous of ACE gene and diabetic patients developed nephropathy. In addition, individuals with D allele have higher levels of serum ACE compared to those having I allele. ACE gene polymorphism and serum ACE level may serve as a susceptibility biomarker for nephropathy in type 2 diabetic patients. 

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Author Biographies

Olfat Gamil Shaker, Cairo University

Medical Biochemistry Dept., Faculty of Medicine

Manal Fouad Ismail, Cairo University

Biochemistry Dept., Faculty of Pharmacy

Esmat Ashour, National Research Centre, Dokki, Giza, Egypt

Biochemistry Dept.

Heba Mourad Yousif, Cairo University

Internal Medicine Dept., Faculty of Medicine

Mie Afify, National Research Centre, Dokki, Giza

Biochemistry Dept.

Weaam Gouda, National Research Centre, Dokki, Giza

Biochemistry Dept.

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Published

2013-12-01

How to Cite

Shaker, O. G., Ismail, M. F., Ashour, E., Yousif, H. M., Afify, M., & Gouda, W. (2013). ACE gene polymorphism and serum ACE level with Progression of Nephropathy in Type 2 Diabetic Patients. JOURNAL OF ADVANCES IN CHEMISTRY, 9(3), 2023–2032. https://doi.org/10.24297/jac.v9i3.1009

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