Laminaria Japonica polysaccharide promotes the expression of insulin receptor and reduces insulin resistance in diabetes mellitus mice

Authors

  • Li Xiao-dan Institute of Integrative Medicine, Qingdao University, Qingdao 266021
  • Ning Chi Institute of Integrative Medicine, Qingdao University, Qingdao 266021
  • Shuai Li School of Chemistry and Environment Engineering, Qingdao University, Qingdao 266071
  • Guo Yu-xuan Department of Oceanology, Hehai University, Nanjing 210098
  • Duan De-Lin Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071
  • Guo Yun-liang Institute of Integrative Medicine, Qingdao University, Qingdao 266021

DOI:

https://doi.org/10.24297/jab.v4i3.3747

Keywords:

LJPS, Type 2 Diabetes, Insulin, Insulin receptor, Mice

Abstract

The aim of experiment was to investigate the effect of Laminaria Japonica polysaccharide (LJPS) on the expression of insulin receptor (InsR) and its hypoglycemic activity in type 2 diabetes mellitus mice. Type 2 diabetes mellitus models were established by feeding high fatty forage and injecting alloxan in 40 healthy male mice. The LJPS was applied as additive in physiological saline to treat the mice by intragastric administraton. The levels of fasting blood glucose (FBG) were detected by automatic blood glucose device. The tissue morphology of brainstem, liver and pancreas were stained by Hematoxylin-eosin (HE) assay and observed under light microscopy. The expression of InsR was determined by immunohistochemisty and Western blot, and the expression of InsR mRNA was detected by RT-PCR. The results indicated after treated with LJPS, the serum fasting (FBG) levels were significantly decreased than those in model group (P<0.05). The morphology and structure of liver and pancreas tissue improved than those in model group. The expressions of InsR mRNA and protein were significantly higher than those in model group (P<0.05). These results suggest that LJPS could play a hypoglycemic effect by promoting the expression of InsR in liver and pancreatic tissue to lessen insulin resistance (IR).

Downloads

Download data is not yet available.

Downloads

Published

2014-05-04

How to Cite

Xiao-dan, L., Chi, N., Li, S., Yu-xuan, G., De-Lin, D., & Yun-liang, G. (2014). Laminaria Japonica polysaccharide promotes the expression of insulin receptor and reduces insulin resistance in diabetes mellitus mice. JOURNAL OF ADVANCES IN BIOLOGY, 4(3), 477–484. https://doi.org/10.24297/jab.v4i3.3747

Issue

Section

Articles