The influence of picroside II on the expressions of NSE, S100B and MBP in cerebral ischemia of rats

Abstract

The aim is to study the neuroprotection and optimize the therapeutic dose and time window of picroside II by orthogonal test in cerebral ischemic injury in rats. The forebrain ischemia models were established by bilateral common carotid artery occlusion (BCCAO) methods. The successful models were randomly grouped according to orthogonal experimental design and treated by injecting picroside II intraperitonenally with different dose at different ischemic time. The concents of neuron-specific enolase (NSE), neuroglial marker protein S100B and myelin basic protein (MBP) in brain tissue were determined by enzyme linked immunosorbent assay to evaluated the therapeutic effect of picroside II in cerebral ischemic injury. The Results proved that the best therapeutic time window and dose of picroside II in cerebral ischemic injury were (1) ischemia 1.5h with 10mg/kgbody weight according to the concentration of NSE in brain tissue; (2) ischemia 1.5h with 20mg/kg according to the concentration of S100B; and (3) ischemia 1.5h with 10mg/kg according to the concentration of MBP. It is Concluded that the optimized therapeutic dose and time window is injecting picroside II intraperitonenally with 10-20mg/kg body weight at ischemia 1.5h in cerebral ischemic injury in rats according to the principle of lowest therapeutic dose with longest time window.