Ameliorative effect of vitamin C on cypermethrin-induced hepatotoxicity and renal malfunction of adult male rats.

Nessrin  Kheirallah; Hussein K.  Hussein; Horeya Abd El  Gawad; Basma  Youssef

doi:10.24297/jab.v14i.9007

Authors

Nessrin Kheirallah Zoology Department, Faculty of Science, Alexandria University, Alexandria- Egypt
Hussein K. Hussein Zoology Department, Faculty of Science, Alexandria University, Alexandria- Egypt
Horeya Abd El Gawad Zoology Department, Faculty of Science, Alexandria University, Alexandria- Egypt.
Basma Youssef Zoology Department, Faculty of Science, Alexandria University, Alexandria- Egypt.

DOI:

https://doi.org/10.24297/jab.v14i.9007

Keywords:

kidney function , liver enzymes, antioxidant parameters, vitamin C, cypermethrin

Abstract

Cypermethrin is a widely used insecticide belongs to type II pyrethroids. It is highly used in developing countries to control many species of insects as it plays an important role in pest control. Vitamin C is an important intracellular antioxidant against insecticides. The aim of the present study is to analyze the toxic effects of cypermethrin (CYP) and the positive effect of vitamin C (VC) with graded doses (0 CYP, 200 VC, 12 CYP and 12 CYP+200 VC mg/kg body weight of male rats/day) for 30 days on liver and kidney functions. Moreover, antioxidant enzymes, oxidative stress markers, glycogen and glucose levels and also serum lipid profile were studied. Also, this study aimed to evaluate the possible protective role of vitamin C against cypermethrin toxicity

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References

Abdou H.M., Hussien H.M. Yousef M.I. (2012). Deleterious effects of cypermethrin on rat liver and kidney: protective role of sesame oil. J. Environ. Sci. Health, Part B: Pesticides, Food Contaminants and Agricultural Wastes, 47 (4), 306-14.

https://doi.org/10.1080/03601234.2012.640913

PMid:22428892

Afolabia O.k., Aderibigbe F.A., Folarin D.T., Arinola A., Wusu A.D. Heliyon E. (2019). Oxidative stress and inflammation following sub-lethal oral exposure of cypermethrin mitigating potential of epicatechin, Heliyon, 5:1-7.

https://doi.org/10.1016/j.heliyon.2019.e02274

PMid:31440603 PMCid:PMC6700339

Ambali S.F., Abubakar A.T., Shittu, M., Yaqub L.S., Anafi S.B. Abdullahi A. (2010). Chlorpyrifos-induced alteration of hematological parameters in Wistar rats: Ameliorative effect of Zinc. J. Environ. Toxicol., 4:55-

https://doi.org/10.3923/rjet.2010.55.66

Anand S.S., Bruckner J.V., Haines W.T., Muralidhara S., Fisher J.W., Padilla S. (2006). characterization of deltamethrin metabolism by rat plasma and liver microsomes. Toxicol Appl. Pharmacol., 212:156-166.

https://doi.org/10.1016/j.taap.2005.07.021

PMid:16169030

Annae R., Creppy E.E. (2001). Lipid peroxidation as pathway of aluminium cytotoxicity in human skin fibroblast cultures: prevention by superoxide dismutase+catalase and vitamin E and C. Hum. Exp. Toxicol., 20:477-81

https://doi.org/10.1191/096032701682693053

PMid:11776410

Arrigoni o., De Tulio M.C. (2002). "Ascorbic acid: much more than just an antioxidant" Biochim. Biophys. Acta., 1569,1-9.

https://doi.org/10.1016/S0304-4165(01)00235-5

Aslam F., Khan A., Khan M.Z., Sharaf S., Gul S.T., Saleemi M.K. (2010). Toxico-pathological changes induced by cypermethrin in broiler chicks: Their attenuation with Vitamin E and selenium. Exp. Toxicol. Pathol., 62: 441-450.

https://doi.org/10.1016/j.etp.2009.06.004

PMid:19581076

Atessahin A., Yilmaz S., Karahan I., Tasmedir P.B. (2005). "The effects of vitamin E and selenium on cypermethrin induced oxidative stress in rats". Turk. J.Vet. Anim. Sci, 29: 385-91.

Attia A.M., Nasr H.M. (2009). Evaluation of protective effect of omega-3 fatty acids and selenium on paraquat intoxicated rats. Slovak J. Anim. Sci, 42: 180-187.

Badgujar N.N., pawar G.A., chandratre A., Telang A., sharma F. (2015). Induced oxidative stress in kidney and brain of mice :protective effect of vitamin E and vitamin C, pestic: Biochem. Physiol.,118: 10-18.

https://doi.org/10.1016/j.pestbp.2014.10.013

PMid:25752424

Bartels H., Bohmer M., Heierli C. (1972). serum creatinine determination without protein precipitation clinical chimica acta: Int J clinical chem, 37:193-197.

https://doi.org/10.1016/0009-8981(72)90432-9

Bashandy S.A., Alwasel, S.H. (2011). "Carbon Tetrachlo-ride-Induced Hepatotoxicity and Nephrotoxicity in Rats: Protective Role of Vitamin C,"J. Pharmacol. Toxicol., 6:(3), 283-292.

https://doi.org/10.3923/jpt.2011.283.292

Bernet D., Schmidt H., Wahli T., Burkhardt-Holm P. (2001). Effluent from a sewage treatment works causes changes in serum biochemistry of brown trout (SalmotruttaL.). Ecotoxicol. Environ. Saf, 48:140-147.

https://doi.org/10.1006/eesa.2000.2012

PMid:11161688

Bessoy O.A., Lowry O.H., Bruck M.J. (1946). A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. J. Biot chem, 164: 321-329.

https://doi.org/10.1016/S0021-9258(18)43072-4

Bhanu A.P., Deepak M. (2015). Impact of cypermethrin on biochemical aspects of clinical importance in the blood of freshwater fish Cyprinus carpio. J. Entmol. Zool. Stud. 3 (1): 126-128.

Bhatti G. K., Sidhu I.P.S., Saini N. K., Puar S.K., Singh G., Bhatti J.S. (2014). Ameliorative role of melatonin against cypermethrin induced hepatotoxicity and impaired antioxidant defense system in Wistar rats. J. Environ. Sci., 8 (1).39-48.

https://doi.org/10.9790/2402-08163948

Bhushan B., Pande S., Saxena N., and Saxena P. (2013). Serum biochemical responses under stress of cypermethrin in albino rat. Environ. Expe. Biol, 11: 81-89.

Bishop M.L., Michael L., Edward P., Fody Larry E. (2000). Clinical Chemistry: Principles, Procedures, Correlat ions (4thed.). Philadelphia, PA: Lippincott Williams & Wilkins Sigma Technical Bulletin No. 540.

El-Gohary M., Awara W.M., Nassar S., Hawas S. (1999). Deltamethrin-induced testicular apoptosis in rats: the protective effect of nitric oxide synthase inhibitor. Toxicol, 132:1-8.

https://doi.org/10.1016/S0300-483X(98)00114-0

Eraslan G., Kanbur M., Silici S., Altinordulu S., Karabacak, M. (2008). Effecs of Cypermethrin on Some Biochemical Changes in Rats: The Protective Role of Protective Role of Propolis. Faculty of Veterinary Medicine, 57 (5). 453-460.

https://doi.org/10.1538/expanim.57.453

PMid:18946182

Essawy A.E., Abdel-Wahab W.M., Sadek I. A., Khamis O.M. (2018). Dual protective effect of ginger and rosemary extrac against CCl4-induced hepatotoxicity in rats. Environ. Sci. Pollut. Res. Int., 25(20).19510-19517.

https://doi.org/10.1007/s11356-018-2129-5

PMid:29730760

Fawcett J.K., Soctt J.E. (1960). A rapid and precise method for the determination of urea. J. clin. pathol, 13(2),156-159.

https://doi.org/10.1136/jcp.13.2.156

PMid:13821779 PMCid:PMC480024

Garba S.H., Adelaiye A.B., Mshelia L.Y. (2007). Histopathological and biochemical changes in the rats' kidney following exposure to a pyrethroid based mosquito coil. J Appl. Sci. Res., 3:1788-1793.

Gaziano J.M., Glynn W.G., Christen T., Kurth C., Belanger, J., MacFadyen V., Bubes J.E., Manson H.D., Sesso J.E., Jomova K., Vondrakova D., Lawson M., valko M. (2010). Metals, oxidative stress and neurodegenerative disorders. Molec. Cell. Biochem. ,345(1-2),91-104.

https://doi.org/10.1007/s11010-010-0563-x

PMid:20730621

Ghassemi F., Nael M.K., Danshvar M. (2015). The effects of Deltamethrin on liver function in female rats. WALIA journal 31(S1): 54-58.

Goel A., Dani V., Dhawan D.K. (2005). Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos-induced toxicity. Chemico-Biological Interactions, 156(2-3),131-140.

https://doi.org/10.1016/j.cbi.2005.08.004

PMid:16169541

Goldberg D.M. Spooner R.J. (1983) Assay of Glutathione Reductase. In: Methods of Enzymatic Analysis, 3rd Edition, Vol. 3, Verlog Chemie, Deerfiled Beach, 258-265.

Gonzalez-Romo P., Sanchez-Nieto S., Gavilanes-Ruiz M. (1992). A modified colorimetric method for the determination of orthophosphate in the presence of high ATP concentrations. Anal. biochem., 200:235-238.

https://doi.org/10.1016/0003-2697(92)90458-J

Gordon M.H. (2012). Significance of Dietary Antioxidants for Health. Int. J. Mol. Sci., 13:173-179.

https://doi.org/10.3390/ijms13010173

PMid:22312245 PMCid:PMC3269679

Gornall A.J., Bardawill, C.J., David, M.M. (1949). Determination of serum proteins by means of the biuret reaction. J. Biol. Chem.,177:571-66.

https://doi.org/10.1016/S0021-9258(18)57021-6

Grewal K.K., Sandhu G.S., RanjitKaur Brar R.S., Sandhu, H.S. (2010). Toxic impacts of cypermethrin on behavior and histology of certain tissues of albino rats. Toxicol. Int. 17, 94-98.

https://doi.org/10.4103/0971-6580.72679

PMid:21170254 PMCid:PMC2997464

Howell D.C. (1995). fundamental statistics for the behavioral sciences 93rd ed.) Duxbury press. An Imprint of wads worth publishing company Belmont. California. 163-166.

Huang Y.L., Yu F.Z., Si Y.W., Shi N. (2007). related factor 2 activation and hemeoxygenase-1 induction by tert-butylhydroquinone protect against-deltamethrin-mediated oxidative stress in pc12 cells. Chem. Res. Toxicol., 20:1242-51.

https://doi.org/10.1021/tx700076q

PMid:17676812

Hussein H.K., Elnaggar M.H., Al-Dailamy, J.M. (2012). Protective role of Vitamin C against hepatorenal toxicity of fenvalerate in male rats. Global Advanced Research J. Environ. Sci. Toxicol., 1(4). 060-065.

Hussien H.M., Abdou H.M., Yousef M.I. (2013). Cypermethrin induced damage in genomic DNA and histopathological changes in brain and haematotoxicity in rats: The protective effect of sesame oil. Brain Res. Bull., 92 76-83.

https://doi.org/10.1016/j.brainresbull.2011.10.020

PMid:22085743

Kalender S., Kalender Y., Durak D., Ogutcu A., Uzunhisarcikli M., Sitki Cevrimli B., Yildirim, M. (2007). Methyl parathion induced nephrotoxicity in male rats and protective role of vitamins C and E. Pes Biochem. Physiol, 88(2). 213-218.

https://doi.org/10.1016/j.pestbp.2006.11.007

Kalender S., Ogutcu A., Uzunhisaracikli M., Acikzoz F., Durak D., Ulusoy Y., Kalender,Y.(2005). Diazonin induced hepatotoxicity and protective effect of vitamins E on some biochemical indices and ultra-structural changes. Toxicol., 21: 197-206.

https://doi.org/10.1016/j.tox.2005.03.007

PMid:15925023

Kashif S.M., Zaidi R., Banu N. (2004). Antioxidant potential of vitamins A, E and C in modulating oxidative stress in rat brain. Clinica Chimica Acta, 340(1-2):229-33.

https://doi.org/10.1016/j.cccn.2003.11.003

PMid:14734217

Khurshid A. (2003). Cypermethrin, a Pyrethroid Insecticide Induces Teratological and Biochemical changes in Young Chick Embryos. Pak. J.Biol. Sci.,6:1698-1705Medicine, 6th edition Mosby-Year Book Inc., St Louis., pp: 309-330

https://doi.org/10.3923/pjbs.2003.1698.1705

Kocaman A.Y., Topaktas M. (2009). The in vitro genotoxic effects of acommercial formulation of alpha-cypermethrin in human peripheral blood lymphocytes. Environ. Mol. Mutagen., 50: 27-36.

https://doi.org/10.1002/em.20434

PMid:19031422

Korkmaz N., Cengiz EI., Unlu E., Uysal E., Yanar M. (2009). Cypermethrin induced histopathological and biochemical changes in Nile tilapia (Oreochromis niloticus) and the protective and recuperative effect of ascorbic acid. Environ. Toxicol. Pharmacol, 28(2).198-205.

https://doi.org/10.1016/j.etap.2009.04.004

PMid:21784003

Kulpmann W.R. (1991). Reference methods for the determination of sodium, potassium, pH and blood gases with ion-selective electrodes. Eur. J. Clin. Chem. Clin. Biochem., 29(4). 263-7.

https://doi.org/10.1515/cclm.1991.29.4.263

PMid:1868132

Kurowska E.M., Spence J.D., Jordan J., Wetmore S., Freeman D.J., Piche L.A., Serratore P. (2000). HDLcholesterol raising effect of orange juice in subjects with hypercholesterolemia. Am. J.Clin. Nutr., 72:1095-1100.

https://doi.org/10.1093/ajcn/72.5.1095

PMid:11063434

Lamberth C., Jeanmart S., Luksch T., and Plant A. (2013). "Current Challenges and Trends in the Discovery of Agrochemicals". Sci. 341 (6147). 742-6.

https://doi.org/10.1126/science.1237227

PMid:23950530

Latchoumycandane C and Mathur PP (2002). Induction of oxidative stress in the rat testis after short-term exposure to the organochlorine pesticide methoxychlor. Arch Toxicol.,76:692-8.

https://doi.org/10.1007/s00204-002-0388-9

PMid:12451445

Lidova A.S., kouba A., and velisek j. (2016). The effect of cypermethrin on oxidative stress and antioxidant biomarkers in marbled crayfish (procambarus fallax f.virginalis), Neuroendocrinol. Lett.37,53-59.

Manna S., Bhattacharyya D., Mandal T.K., Das, S. (2004). Repeated dose toxicity of alfa-cypermethrin in rats. J. Vet. Sci. 5: 241-245.

https://doi.org/10.4142/jvs.2004.5.3.241

PMid:15365239

Manna s., Bhattacharyya D., Mandal T.K., Das S. (2006). sub-chronic toxicity study of alfa-cypermethrin in rats. Iranian J. Pharmacol. :u pt 5:163-6.

Moshage H., Kok B., Huizenga J.R. (1995). Nitritc and nitratc determination in plasma: A critical evaluation. Clin. chem. ,41:892-896.

https://doi.org/10.1093/clinchem/41.6.892

PMid:7768008

Nair R.R., Abraham M.J., Nair N.D., Lalithakunjamma C.R., Aravindakshan, C.M. (2010). Hematological and biochemical profile in sublethal toxicity of cypermethrin in rats. Int. J. Biol. Med. Res.1:211-214.

https://doi.org/10.4103/2231-0738.84211

Newairy A. A., Abdou H.M. (2013). Effect of propolis consumption on hepatotoxicity and brain damage in male rats exposed to chlorpyrifos. African J. Biotech., 12 (33). 5232-5243.

https://doi.org/10.5897/AJB12.2797

Nicholas V., carroll R., Longley W., Joseph H.R. (1956). The determination of glycogen in liver and muscle by use of anthron reagent. J. biol. Chem., 220:583-593.

https://doi.org/10.1016/S0021-9258(18)65284-6

Nishikimi M., Roa N.A.,Yogi, K. (1972). The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem. Biophysical. Res. Communications, 46(2), 849 - 854.

https://doi.org/10.1016/S0006-291X(72)80218-3

Okawa H., Ohishi N., Yagi K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical. Biochem., 95:351-358.

https://doi.org/10.1016/0003-2697(79)90738-3

Padayatty S.J., Katz A., Wang Y., Eck P., Kwon O., Lee J.H (2003). Vitamin C as an antioxidant: evaluation of its role in disease prevention. Jam Coll Nutr22(1).18-35.

https://doi.org/10.1080/07315724.2003.10719272

PMid:12569111

Patterson R.A., Horsley E.T., Leake D.S. (2003). The prooxidant and antioxidant properties of human serum ultra filtrates toward LDL: important role of uric acid. J Lipid Res., 44:512-521.

https://doi.org/10.1194/jlr.M200407-JLR200

PMid:12562831

Rajeshkumar N.V., Kuttan R. (2003). Modulation of carcinogenic response and antioxidant enzymes of rats administered with 1,2-dimethylhydrazine by picroliv. Cancer Lett,191:137-43.

https://doi.org/10.1016/S0304-3835(02)00203-3

Ravel R. (1995). Clinical Application of Laboratory Data, In: Clinical Laboratory

Reddy A.T., Ayyanna K., Yellamma K. (1991). Cypermethrin induced modulation in lipid metabolism of freshwater teleost, Tilapia mossambica. Biochem Int, Mar, 23(5).963-7.

Rosalki S.B., Foo A.Y., Burlina A., prellwitz W., Stieber p., Neumeier D., Bodenmuller H. (1993). Multicenter evaluation of Iso-ALP test kit for measurement of bone alkalin phosphatase activity in serum and plasma. Clinical chem. ,39(4),648-652

https://doi.org/10.1093/clinchem/39.4.648

Sakr S.A., Albarakai Y. (2014). Effect of cinnamon on cypermethrin induced nephrotoxicity in albino rats. Int. J. Adv. Res. 2 (7). 578-586.

Sankar P., Telang A.G., Manimaran A. (2010). Curcumin protects against cypermethrin induced genotoxicity in rats. Environ. Toxicol. Pharmacol. 30,289-291.

https://doi.org/10.1016/j.etap.2010.07.005

PMid:21787662

Sankar P., Telang A.G., Manimaran A. (2011). Effect of piperine on cypermethrininduced oxidative damage in rats. J. Veterinar. Sci. Technol. 2, 105.

https://doi.org/10.4172/2157-7579.1000105

Sankar P., Telang A.G., Manimaran A. (2012). Protective effect of curcumin on cypermethrin-induced oxidative stress in Wistar rats. Exp. Toxicol. Pathol. 64 (5).487-93.

https://doi.org/10.1016/j.etp.2010.11.003

PMid:21130633

Saxena P., Saxena, A.K. (2010). Cypermethrin induced biochemical alteration in the blood of albino rats. Jordan J.Biol.sci.3:111-114.

Sharma R.K., Bhardwaj S. (2018). protective effects of tribulus terrestris and Vitamin C on Permethrin Induced Oxidative Stress in Goat Testis. (SJLS)., 3(3).322-328

Sharma P., Firdous S., Singh R. (2014). Neurotoxic effect of cypermethrin and protective role of resveratrol in Wistar rats. International J. of Nutrition, Pharmacol, Neurolo. Disease,s 4 (2), 104-111

https://doi.org/10.4103/2231-0738.129598

Matsumoto S., Häberle J., Kido J., Mitsubuchi H., Endo F., Nakamura K. (2019). Urea cycle disorders-update. Journal of Human Genetics, 64,833-847

https://doi.org/10.1038/s10038-019-0614-4

PMid:31110235

Shirzad H., Taji F., Rafieian-Kopaei M. (2011). Correlation between antioxidant activity of garlic extracts and WEHI-164 fibrosarcoma tumor growth in BALB/c mice. J. Med. Food, 14: 969-74.

https://doi.org/10.1089/jmf.2011.1594

PMid:21812650

Siman C.M., Eriksson U.J. (1997). Vitamin C supplementation of the maternal diet reduces the rate of malformation in the offspring of diabetic rats. Diabetologia, 40(12),1416-1424.

https://doi.org/10.1007/s001250050844

PMid:9447949

Singh A.K., Tiwari M.N., Prakash O., Singh M.P. (2012). A Current Review of Cypermethrin-Induced Neurotoxicity and Nigrostriatal Dopaminergic Neurodegeneration. Curr. Neuropharmacol.,10 (1). 64-71.

https://doi.org/10.2174/157015912799362779

PMid:22942879 PMCid:PMC3286848

Soliman M.M., Attia H.F., Abou El-Ella G.A. (2014). Genetic and histopathological alterations induced by cypermethrin in rat kidney and liver: Protection by sesame oil. Int. J. Immunopathol. Pharmacol, 1-13.

Tao T.Y., Wei L.Z., Yang Y., Tao, Z., Zhwo, Y. (2008). Affects of alpha and theta cypermethrin insecticide on transient outward potassium current in rat hippocampal CA3 neurons. Pesticide Biochem. Physiol., 90:1-7.

https://doi.org/10.1016/j.pestbp.2007.07.002

Tawfik M.S., Al-Badr N. (2012). Adverse Effects of Monosodium Glutamate on Liver and Kidney Functions in Adult Rats and Potential Protective Effect of Vitamins C and E. Food. Nutr. Sci. 3:651- 659.

https://doi.org/10.4236/fns.2012.35089

Thomas L. (1998). Alanine aminotransferase (ALT), Aspartate aminotransferase (AST). In: Thomas L. editor. Clinical laboratory diagnostics. 1st edition. TH-Books Verlagsgesellschaft: 55-56.

Tietz N.W. (1976). Fundamentals of Clinical Chemistry. W.B.Sanuders Co., Philadelphia, USA.

Tietz N. W. (1983). Clinical Guide to Laboratory Tests. W. B. Saunders Co.. teleost: A Review. Zool. Pol, 47(3/4). 49-64.

Tiwari M.N., Singh A.K., Israr A., Upadhyay G., Singh D., Patel D.K., Singh C., Prakash O., Singh M.P. (2010). Effects of cypermethrin on monoamine transporters, xenobiotic metabolizing enzymes and lipid peroxidation in the rat nigrostriatal system. Free Radic. Res, 44: 1416-1424.

https://doi.org/10.3109/10715762.2010.512041

PMid:20815782

Trinder P. (1969). Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor, Ann. Clin. Biochem, 6: 24-25.

https://doi.org/10.1177/000456326900600108

Vaithinathan S., Saradha B., Mathur P.P. (2009). Methoxychlor-induced alteration in the levels of HSP70 and clusterin is accompanied with oxidative stress in adult rat testis. J Biochem Mol Toxicol., 23:29-35

https://doi.org/10.1002/jbt.20262

PMid:19202561

Veerappan M., Hwang I., Pandurangan M. (2012). Effect of cypermethrin,carbendazim and their combination on male albino rat serum. Int. J. Clin. Exp. Pathol, 93: 361-369.

https://doi.org/10.1111/j.1365-2613.2012.00828.x

PMid:22974217 PMCid:PMC3444992

Yaji A.J., Auta J., Oniye S.J., Adakole J.A., Usman J.I. (2011). Effects of cypermethrin on Behaviour and biochemical indices of Freshwater fish Oreochromis niloticus. Electronic journal of Environment, Agriculture and food chemistry,10 (2), 1927-34.

Yousef, M.I., El-Deerdash F.M., Kamel K.I., Al- Salhen K.S. (2003). Changes in some haematological and biochemical indices of rabbits induced by is flavones and cypermethrin. Toxicol.189:223-234.

https://doi.org/10.1016/S0300-483X(03)00145-8