The Curative Effect of Berberine Nanoparticles and Cisplatin Combination Therapies Against Hepatocarcinogenesis-Induced By N-Nitroso-Diethylamine In Male Rat


  • Nema Abdelhameed Mohamed Alexandria University, Egypt
  • Awatef Mohamed Ali Alexandria University, Egypt
  • Doaa Ahmed Ghareeb Alexandria University, Egypt
  • Adham Rashed Mohamed Alexandria University, Egypt
  • Yasmin Mohamed Elmokhtar Alexandria University, Egypt



Berberine Nanoparticles, Cisplatin, N-Nitrosodiethylamine, Liver, Rats


This study aimed to investigate whether berberine nanoparticles (BBR-NPs) and/or cisplatin supplementation could prevent hepatocarcinogenesis-induced by N-nitroso-diethylamine (DENA) in male rats. Male Wistar albino rats were divided into five groups; Group 1: Control; Group 2: DENA-CCl4; Group 3: DENA-CCl4+Cisplatin; Group 4: DENA-CCl4+BBR-NPs; Group 5: DENA-CCl4+Cisplatin+BBR-NPs. DENA-CCl4 significantly increase AST, ALT, ALP, LDH, GGT, AFP activities and total bilirubin, while, 5, NT,  total protein and albumin decreased. DENA-CCl4 treatment caused increment in MDA levels and reduction in SOD, CAT, GPx and GSH in liver tissues. Moreover, DENA-CCl4 increase the gene expression of ADAM17 and TNF-a however,  P53 was declined. In addition, DENA-CCl4 caused severe histopathological lesions in the liver tissue. Interestingly, administration of berberine nanoparticles alone or in combination with cisplatin improves the hepatocarcinogenesis induced by DENA-CCl4 on the physiological, biochemical, molecular and histological levels by decreasing oxidative stress and preserving gene expression of ADAM17, TNF-I and P53. The present findings suggest that BBR-NPs with cisplatin might offer a promising strategy for the prevention of liver cancer.


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Author Biographies

Nema Abdelhameed Mohamed, Alexandria University, Egypt

Zoology Department, Faculty of Science, Alexandria University, Egypt

Awatef Mohamed Ali, Alexandria University, Egypt

Zoology Department, Faculty of Science, Alexandria University, Egypt

Doaa Ahmed Ghareeb, Alexandria University, Egypt

Biological Sciences Department, Faculty of Science, Beirut Arab University, Biochemistry Department, Faculty
of Science, Alexandria University, Egypt

Adham Rashed Mohamed, Alexandria University, Egypt

Physiology Department, Faculty of Medicine, Alexandria University, Egypt

Yasmin Mohamed Elmokhtar, Alexandria University, Egypt

1Zoology Department, Faculty of Science, Alexandria University, Egypt


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How to Cite

Mohamed, N. A., Ali, A. M., Ghareeb, D. A., Mohamed, A. R., & Elmokhtar, Y. M. (2018). The Curative Effect of Berberine Nanoparticles and Cisplatin Combination Therapies Against Hepatocarcinogenesis-Induced By N-Nitroso-Diethylamine In Male Rat. JOURNAL OF ADVANCES IN BIOLOGY, 11, 2180–2200.