Picroside II Could Protect The Cerebral Ischemic Injury by Reducing The Content of Free Radical and Enhancing The Activity of Antioxidase in Rats
DOI:
https://doi.org/10.24297/jab.v3i2.6560Keywords:
Cerebral ischemia; Free radicals; Anti-oxidase; Picroside II; Therapeutic dose; Time window; RatsAbstract
The aim is to optimize the anti-oxidation and the drug dose and medication time of picroside II by orthogonal test in cerebral ischemia in rats. The forebrain ischemia models were established by bilateral common carotid artery occlusion (BCCAO) methods. The successful models were randomly grouped according to orthogonal experimental design and injected picroside II intraperitonenally with different dose at different ischemic time for treatment. The contents of malondialdehyde (MDA), nitric oxide (NO) and hydrogenperoxide (H2O2) in brain tissue were respectively determined by thiobarbituric acid assay, nitratase reduase assay and chemiluminescence immunoassay. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSHPx) and catalase (CAT) in brain tissue were respectively determined by xanthinoxidase assay, chemical colorimetry and chemiluminescence immunoassay. The results indicated that the best therapeutic time window and dose of picroside II in cerebral ischemic reperfusion injury was (1) ischemia 1.5h with 10mg/kg, 1.5h with 20mg/kg and 1.5h with 20mgkg body weight according to the contents of MDA, NO and H2O2 in brain tissue; (2) ischemia 1.5h with 20mg/kg, 2.0h with 20mg/kg and 1.5h with 10mg/kg body weight according to the activities of SOD, GSHPx and CAT in brain tissue. From the principle of lowest therapeutic dose with longest time window, the optimized therapeutic dose and time window is injecting picroside II intraperitonenally with 10-20mg/kg at 1.5-2.0h after cerebral ischemic injury.
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[1] Ito, Y., Ohkubo, T., Asano, Y., Hattori, K., Shimazu, T., Yamazato, M., Nagoya, H., Kato, Y., Araki, N. Nitric oxide production during cerebral ischemia and reperfusion in eNOS and nNOS-knockout mice. J Curr Neurovasc Res, 2010, 7, 23-31.
[2] Choi, D.W. Glutamate Neurotoxicity and Disease of the Nervous System. Neurol, 1998, 1, 623-634.
[3] Brown, G.C. Nitric oxide and neuronal death. Nitric Oxide, 2010, 23, 153-165.
[4.]Perluigi M, Di Domenico F, Giorgi A, Schininà ME, Coccia R, Cini C, Bellia F, Cambria MT, Cornelius C, Butterfield DA, Calabrese V. Redox proteomics in aging rat brain: involvement of mitochondrial reduced glutathione status and mitochondrial protein oxidation in the aging process. J Neurosci Res, 2010, 88(16): 3498-3507.
[5] Rey B, Roussel D, Teulier L, Eyenga P, Degletagne C, Belouze M, Duchamp C. Functional argument for the existence of an avian nitric oxide synthase in muscle mltochondria: effect of cold acclimation. FEBS Lett, 2011, 585(1):173-177.
[6] Asano H, Horinouchi T, Mai Y, Sawada O, Fujii S, Nishiya T, Minami M, Katayama T, Iwanaga T, Terada K, Miwa S. Nicotine- and tar-free cigarette smoke induces cell damage through reactive oxygen species newly generated by PKC-dependent activation of NADPH oxidase. J Pharmacol Sci, 2012, 118(2):275-87.
[7] Yan TH, Jia Y, Yang W, Wang QJ. The protective effect of rhodiola glucoside in focal cerebral ischemia/ reperfusion injury in rats. Chin Pharmacol Bull, 2008, 24(11):1521-1524.
[8] Wang JY, Yu R, Yao MH. The protective effect of rhodiola glucoside in cerebral ischemia reperfusion injury in rats. J Chin med, 2010, 25 (3):456-459.
[9] Lukic-Panin V, Deguchi K, Yamashita T, Shang J, Zhang X, Tian F, Liu N, Kawai H, Matsuura T, Abe K. Free radical scavenger edaravone administration protects against tissue plasminogen activator induced oxidative stress and blood brain barrier damage. Curr Neurovasc Res, 2010, 7(4):319-329.
[10] Noh SJ, Lee SH, Shin KY, Lee CK, Cho IH, Kim HS, Suh YH. SP-8203 reduces oxidative stress via SOD activity and behavioral deficit in cerebral ischemia. Pharmacol Biochem Behav, 2011, 98(1):150-154.
[11] Raza SS, Khan MM, Ashafaq M, Ahmad A, Khuwaja G, Khan A, Siddiqui MS, Safhi MM, Islam F. Silymarin protects neurons from oxidative stress associated damages in focal cerebral ischemia: a behavioral, biochemical and immunohistological study in Wistar rats. J Neurol Sci, 2011, 309(1-2): 45-54.
[12] Tseng YT, Hsu YY, Shih YT, Lo YC. Paeonol attenuates microglia-mediated inflammation and oxidative stressinduced neurotoxicity in rat primary microglia and cortical neurons. Shock, 2012, 37(3): 312-318.
[13] Heeba GH, El-Hanafy AA. Nebivolol regulates eNOS and iNOS expressions and alleviates oxidative stress in cerebral ischemia/reperfusion injury in rats. Life Sci, 2012, 90(11-12):388-395.
[14] Tomatsuri N, Yoshida N, Takagi T, Katada K, Isozaki Y, Imamoto E, Uchiyama K, Kokura S, Ichikawa H, Naito Y, Okanoue T. Edavavone, a newly developed radical scavenger protects against ischemia-reperfusion injury of the small intestine in rats. Int J Mol Med, 2004, 13(1):105-107.
[15] Gargouri B, Mansour RB, Abdallah FB, Elfekih A, Lassoued S, Khaled H. Protective effect of quercetin against oxidative stress caused by dimethoate in human peripheral blood lymphocytes. Lipids Health Dis, 2011, 10(2):149-152.
[16] Li P, Matsunaga K, Yamakuni T, Ohizumi Y. Potentiation of nerve growth factor-action by picrosides I and II, natural iridoids, in PC12 cells. Eur J Pharmacol, 2000, 406(2):203-208.
[17] Li P, Matsunaga K, Yamakuni T, Ohizumi Y. Picrosides Iand II selective enhancers of the mitogen-activated protein kinase-dependent signaling pathway in the action of neuritogenic substances on PC12D cells. Life Sci, 2002, 71(15):1821-1835.
[18] Guo MC, Cao Y, Liu JW. The protective effect of picroside II on PC12 cell injury induced by glutamic acid. Chin J Clin Pharm Ther, 2007,12(4):440-443.
[19] Liu JW, Yu YJ, Zheng PY, Zhang XD, Li T, Guo MC. Synergistic protective effect of picroside II and NGF on PC12 cells against oxidative stress induced by H2O2. Pharmacol Rep, 2007, 59(5):573-579.
[20] Li Z, Xu XY, Li Q, Zhang MZ, Shen W. Protective mechanisms of picroside on AQP4 in rat model of MCAO/R. Neural Regen Res, 2010, 5(6):411-416. [21] Li Q, Li Z, Xu XY, Guo YL, Du F. Neuroprotective properties of picroside II in rat model of focal cerebral ischemia. Int J Mol Sci, 2010, 11(11):4580-4590.
[22] Pei HT, Su X, Zhao L, Li HY, Guo YL, Zhang MZ, Xin H. Primary study for the therapeutic dose and Time window of picrosideâ…¡ in treating cerebral ischemic injury in rats. Int J Mol Sci, 2012, 13(3):2551-2562.
[23] Márquez-MartÃn A, Jiménez-Altayó F, Dantas AP, Caracuel L, Planas AM, Vila E.Middle cerebral artery alterations in a rat chronic hypoperfusion model. J Appl Physiol, 2012, 112(3):511-518.
[24] Li DL, Wang XY, Han H, Zhao HG, The effect of progesterone to SOD and GSH-Px in hypoxic ischemic newborn rats. Chin Pharmacol Bull, 2007, 23(2):276-277.
[25] Moralà G, LetechipÃa-Vallejo G, López-Loeza E, Montes P, Hernández-Morales L, Cervantes M. Post ischemic administration of progesterone in rats exerts neuroprotective effects on the hippocampus. Neurosic Lett, 2005, 382 (3):286-290.
[26] Chen HB, Chen TP, Hou GF, Chen XY, ChengSH, Chen XR. The neuroprotective effect of progesterone on the concentration inositol triphosphate in cerebral ischemia/reperfusion in rats. Acta Universitatis Medicinalis Aahui, 2012, 47(5):511-512.
[27] Zhang QL, Sun YB, Wang HY, Song SJ, Bai B. The effect of salvia miltiorrhiza bge to mitochondria damage and cell apoptosis in cerebral ischemic injury in rats. Chin J Pathophysiol, 2010, 26(4):725-729.
[28] Huang JY, Sun JN, Mei SC, Huang JM. The protective effect of bilobalide B in cerebral ischemic injury in rats. Chin Pharmacol Bull, 2008, 24(2):269-272.
[29] Guo C, Tong L, Xi M, Yang H, Dong H, Wen A. Neuroprotective effect of calycosin on cerebral ischemia and reperfusion injury in rats. J Ethnopharmacol, 2012, 144(3):768-774.
[30 Sun YX, Tang Y, Wu AL, Liu T, Dai XL, Zheng QS, Wang ZB. Neuroprotective effect of liquiritin against focal cerebral ischemia/reperfusion in mice via its antioxidant and antiapoptosis properties. J Asian Nat Prod Res, 2010, 12(12): 1051-1060.
[31] Zhao DM, Zhang ZQ, Duan YX, Zhang BZ, Liu QS. The protection research of picrosides II to nerve injury in cerebral ischemia/reperfusion in rats. International Journal of Pharmacy Research, 2010, 37(6):461-468. [32] Sun L, Li XD, Wang L, Qin LH, Guo YL, Zhou Z. The Anti-oxidant effect and the possible mechanism of picrosideâ…¡ in cerebral ischemia reperfusion injury in rats. Neural Regen Res, 2011, 6(15):1141-1146.
[2] Choi, D.W. Glutamate Neurotoxicity and Disease of the Nervous System. Neurol, 1998, 1, 623-634.
[3] Brown, G.C. Nitric oxide and neuronal death. Nitric Oxide, 2010, 23, 153-165.
[4.]Perluigi M, Di Domenico F, Giorgi A, Schininà ME, Coccia R, Cini C, Bellia F, Cambria MT, Cornelius C, Butterfield DA, Calabrese V. Redox proteomics in aging rat brain: involvement of mitochondrial reduced glutathione status and mitochondrial protein oxidation in the aging process. J Neurosci Res, 2010, 88(16): 3498-3507.
[5] Rey B, Roussel D, Teulier L, Eyenga P, Degletagne C, Belouze M, Duchamp C. Functional argument for the existence of an avian nitric oxide synthase in muscle mltochondria: effect of cold acclimation. FEBS Lett, 2011, 585(1):173-177.
[6] Asano H, Horinouchi T, Mai Y, Sawada O, Fujii S, Nishiya T, Minami M, Katayama T, Iwanaga T, Terada K, Miwa S. Nicotine- and tar-free cigarette smoke induces cell damage through reactive oxygen species newly generated by PKC-dependent activation of NADPH oxidase. J Pharmacol Sci, 2012, 118(2):275-87.
[7] Yan TH, Jia Y, Yang W, Wang QJ. The protective effect of rhodiola glucoside in focal cerebral ischemia/ reperfusion injury in rats. Chin Pharmacol Bull, 2008, 24(11):1521-1524.
[8] Wang JY, Yu R, Yao MH. The protective effect of rhodiola glucoside in cerebral ischemia reperfusion injury in rats. J Chin med, 2010, 25 (3):456-459.
[9] Lukic-Panin V, Deguchi K, Yamashita T, Shang J, Zhang X, Tian F, Liu N, Kawai H, Matsuura T, Abe K. Free radical scavenger edaravone administration protects against tissue plasminogen activator induced oxidative stress and blood brain barrier damage. Curr Neurovasc Res, 2010, 7(4):319-329.
[10] Noh SJ, Lee SH, Shin KY, Lee CK, Cho IH, Kim HS, Suh YH. SP-8203 reduces oxidative stress via SOD activity and behavioral deficit in cerebral ischemia. Pharmacol Biochem Behav, 2011, 98(1):150-154.
[11] Raza SS, Khan MM, Ashafaq M, Ahmad A, Khuwaja G, Khan A, Siddiqui MS, Safhi MM, Islam F. Silymarin protects neurons from oxidative stress associated damages in focal cerebral ischemia: a behavioral, biochemical and immunohistological study in Wistar rats. J Neurol Sci, 2011, 309(1-2): 45-54.
[12] Tseng YT, Hsu YY, Shih YT, Lo YC. Paeonol attenuates microglia-mediated inflammation and oxidative stressinduced neurotoxicity in rat primary microglia and cortical neurons. Shock, 2012, 37(3): 312-318.
[13] Heeba GH, El-Hanafy AA. Nebivolol regulates eNOS and iNOS expressions and alleviates oxidative stress in cerebral ischemia/reperfusion injury in rats. Life Sci, 2012, 90(11-12):388-395.
[14] Tomatsuri N, Yoshida N, Takagi T, Katada K, Isozaki Y, Imamoto E, Uchiyama K, Kokura S, Ichikawa H, Naito Y, Okanoue T. Edavavone, a newly developed radical scavenger protects against ischemia-reperfusion injury of the small intestine in rats. Int J Mol Med, 2004, 13(1):105-107.
[15] Gargouri B, Mansour RB, Abdallah FB, Elfekih A, Lassoued S, Khaled H. Protective effect of quercetin against oxidative stress caused by dimethoate in human peripheral blood lymphocytes. Lipids Health Dis, 2011, 10(2):149-152.
[16] Li P, Matsunaga K, Yamakuni T, Ohizumi Y. Potentiation of nerve growth factor-action by picrosides I and II, natural iridoids, in PC12 cells. Eur J Pharmacol, 2000, 406(2):203-208.
[17] Li P, Matsunaga K, Yamakuni T, Ohizumi Y. Picrosides Iand II selective enhancers of the mitogen-activated protein kinase-dependent signaling pathway in the action of neuritogenic substances on PC12D cells. Life Sci, 2002, 71(15):1821-1835.
[18] Guo MC, Cao Y, Liu JW. The protective effect of picroside II on PC12 cell injury induced by glutamic acid. Chin J Clin Pharm Ther, 2007,12(4):440-443.
[19] Liu JW, Yu YJ, Zheng PY, Zhang XD, Li T, Guo MC. Synergistic protective effect of picroside II and NGF on PC12 cells against oxidative stress induced by H2O2. Pharmacol Rep, 2007, 59(5):573-579.
[20] Li Z, Xu XY, Li Q, Zhang MZ, Shen W. Protective mechanisms of picroside on AQP4 in rat model of MCAO/R. Neural Regen Res, 2010, 5(6):411-416. [21] Li Q, Li Z, Xu XY, Guo YL, Du F. Neuroprotective properties of picroside II in rat model of focal cerebral ischemia. Int J Mol Sci, 2010, 11(11):4580-4590.
[22] Pei HT, Su X, Zhao L, Li HY, Guo YL, Zhang MZ, Xin H. Primary study for the therapeutic dose and Time window of picrosideâ…¡ in treating cerebral ischemic injury in rats. Int J Mol Sci, 2012, 13(3):2551-2562.
[23] Márquez-MartÃn A, Jiménez-Altayó F, Dantas AP, Caracuel L, Planas AM, Vila E.Middle cerebral artery alterations in a rat chronic hypoperfusion model. J Appl Physiol, 2012, 112(3):511-518.
[24] Li DL, Wang XY, Han H, Zhao HG, The effect of progesterone to SOD and GSH-Px in hypoxic ischemic newborn rats. Chin Pharmacol Bull, 2007, 23(2):276-277.
[25] Moralà G, LetechipÃa-Vallejo G, López-Loeza E, Montes P, Hernández-Morales L, Cervantes M. Post ischemic administration of progesterone in rats exerts neuroprotective effects on the hippocampus. Neurosic Lett, 2005, 382 (3):286-290.
[26] Chen HB, Chen TP, Hou GF, Chen XY, ChengSH, Chen XR. The neuroprotective effect of progesterone on the concentration inositol triphosphate in cerebral ischemia/reperfusion in rats. Acta Universitatis Medicinalis Aahui, 2012, 47(5):511-512.
[27] Zhang QL, Sun YB, Wang HY, Song SJ, Bai B. The effect of salvia miltiorrhiza bge to mitochondria damage and cell apoptosis in cerebral ischemic injury in rats. Chin J Pathophysiol, 2010, 26(4):725-729.
[28] Huang JY, Sun JN, Mei SC, Huang JM. The protective effect of bilobalide B in cerebral ischemic injury in rats. Chin Pharmacol Bull, 2008, 24(2):269-272.
[29] Guo C, Tong L, Xi M, Yang H, Dong H, Wen A. Neuroprotective effect of calycosin on cerebral ischemia and reperfusion injury in rats. J Ethnopharmacol, 2012, 144(3):768-774.
[30 Sun YX, Tang Y, Wu AL, Liu T, Dai XL, Zheng QS, Wang ZB. Neuroprotective effect of liquiritin against focal cerebral ischemia/reperfusion in mice via its antioxidant and antiapoptosis properties. J Asian Nat Prod Res, 2010, 12(12): 1051-1060.
[31] Zhao DM, Zhang ZQ, Duan YX, Zhang BZ, Liu QS. The protection research of picrosides II to nerve injury in cerebral ischemia/reperfusion in rats. International Journal of Pharmacy Research, 2010, 37(6):461-468. [32] Sun L, Li XD, Wang L, Qin LH, Guo YL, Zhou Z. The Anti-oxidant effect and the possible mechanism of picrosideâ…¡ in cerebral ischemia reperfusion injury in rats. Neural Regen Res, 2011, 6(15):1141-1146.
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Published
2014-01-09
How to Cite
Yang, G., Zhang, R., & Li, X. (2014). Picroside II Could Protect The Cerebral Ischemic Injury by Reducing The Content of Free Radical and Enhancing The Activity of Antioxidase in Rats. JOURNAL OF ADVANCES IN BIOLOGY, 3(2), 219–226. https://doi.org/10.24297/jab.v3i2.6560
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