Comparison of Real time PCR with traditional tools for diagnosis of Trichomonas vaginalis in patient with vaginal discharge at Kut city, Iraq

Authors

  • Abdulsadah Abdulabbas Rahi College of Science, Wasit University, Kut, Iraq
  • Magda A Ali College of Medicine, Wasit University, Kut, Iraq
  • Ali Khamesipour Center of Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran

DOI:

https://doi.org/10.24297/jab.v5i3.5563

Keywords:

T.vaginalis, Vaginal discharge, Human., Real Time PCR

Abstract

Trichomoniasis is a sexually transmitted disease (STD) and most common form of STD which is caused by Trichomonas vaginalis. This is a descriptive analytical study. Vaginal swabs were collected from sixty female patients with suspected Trichomonas vaginalis infection aged 14-53 years old during September 2013 to March 2014 who attended the Gynecology Clinic of Kut city, Iraq. Two swabs were collected from each woman; one for wet mount microscopic examination and the other for Real Time PCR technique. Wet-mount microscopy were positive for T. vaginalis in 5/60 cases (8.33%) and positive Real-time PCR 13/60 (21.67%). The present study revealed that the highest incidence of T.vaginalis infection occurs in age group(24-33) years with the percentage of (46.1%). The sensitivity of Real-time PCR was 100% compared with 38.5% for wet-mount microscopy. The aim of the study was to compare a Real-time PCR assay with wet-mount examination for the detection of T. vaginalis.

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Author Biographies

Abdulsadah Abdulabbas Rahi, College of Science, Wasit University, Kut, Iraq

Department of  Biology,

Magda A Ali, College of Medicine, Wasit University, Kut, Iraq

Department of Microbiology,

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Published

2014-09-12

How to Cite

Rahi, A. A., Ali, M. A., & Khamesipour, A. (2014). Comparison of Real time PCR with traditional tools for diagnosis of Trichomonas vaginalis in patient with vaginal discharge at Kut city, Iraq. JOURNAL OF ADVANCES IN BIOLOGY, 5(3), 697–702. https://doi.org/10.24297/jab.v5i3.5563

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Section

Articles