Generation of hepatocyte from mouse mesenchymal stem cells in Vitro

Abstract

This  study  was designed  to  generate  hepatocyte  cells  from  mesenchymal  stem  cells (MSCs) isolated from mouse bone marrow via using culturing protocols for this goal. Cells of MSCs were isolated by collecting the thigh bone of 50 albino mice, both femur and tibia were collected and cells were flushed from bones and MSCs have been isolated based on the ability of MSCs to adhere on plastic surfaces. MSCs reactivity to CD45, CD90, CD34 and CD105 were tested by immunocytochemistry. Isolated MSCs exhibited positive reactivity  towars CD105 and CD90 cell surface markers. MSCs  were negative for the hematopoietic surface markers including CD34  and CD45. Differentiation into hepatocyte was  induced by adding hepatogenic specific growth factors (Hepatocytes growth factors  (HGF), Fibroblast growth factor4 (FGF4), oncostatin M and dexamethasone) to the differentiation medium. Cells were examined after differentiation protocol and characterized using immunocytochemistry analysis for Albumin and alpha fetoprotein. Positive results were observed  indicating the potential  ability of the isolated bone marrow MSCs to differentiate into Hepatocytes.