Purification and Characterization of Pectinase from Bacillus licheniformis obtained from a Cassava Waste Dump

Authors

  • Joshua Osuigwe Madu
  • Raphael Emuebie Okonji
  • Nkem Torimiro
  • Femi Kayode Agboola

DOI:

https://doi.org/10.24297/jab.v8i3.4552

Keywords:

pectinase, pectin, Bacillus licheniformis, purification

Abstract

This study investigated the properties of pectinase from Bacillus licheniformis. The pectinase secreted by the microorganism was purified using ion exchange and gel filtration chromatography on DEAE-Sephadex and Sephadex G-200 respectively. A purification fold of 10.5, yield of 23.9% and specific activity of 9.47 U/mg was obtained. The characteristics of the enzyme included a native molecular weight of 38 kDa, pH optimum 9.0, temperature stability between 35 and 45ºC; temperature optimum 50°C. Inhibition of the enzyme was observed with metal and chemicals, such as Ba2+, Al3+, Mg2+, EDTA, benzoic acid, tannic acid, citric acid and sodium oxalate, however the enzyme activity was enhanced in the presence of K+ and Na+. Other enzymatic properties was Km values of 1.37, 1.62, and 9.09 mg/ml for polygalacturonic acid, 7.8% methylated pectin and 67% methylated pectin respectively The pectinase produced have some desirable characteristics that could be utilised in many industrial processes.

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Author Biographies

Joshua Osuigwe Madu

Department of Biochemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria and Department of Chemistry, School of Arts and Sciences American University of Nigeria Yola

Raphael Emuebie Okonji

Department of Biochemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife

Nkem Torimiro

Department of Microbiology, Faculty of Science, Obafemi Awolowo University, Ile-Ife

Femi Kayode Agboola

Department of Biochemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife

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Published

2016-01-26

How to Cite

Madu, J. O., Okonji, R. E., Torimiro, N., & Agboola, F. K. (2016). Purification and Characterization of Pectinase from Bacillus licheniformis obtained from a Cassava Waste Dump. JOURNAL OF ADVANCES IN BIOLOGY, 8(3), 1685–1695. https://doi.org/10.24297/jab.v8i3.4552

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Articles