The Effect of Electroacupuncture on the Expression of p53 mRNA in Newborn Rats following Hypoxic-ischemic Encephalopathy
DOI:
https://doi.org/10.24297/jab.v8i3.4494Keywords:
Electroacupuncture, Hypoxic-ischemic Encephalopathy, Neuroprotective effect, Acupoints, p53, RatsAbstract
Aim: To explore the neuroprotective effects of electroacupuncture on the expression of p53 in newborn rats following hypoxic-ischemic encephalopathy (HIE).Method: The hypoxic-ischemic models were made in new-born SD rats aged 7 days by colligating bilateral common carotid artery(CCA). All rats were randomly divided into sham-surgery group, model group and electroacupuncture group (EA), and the successfully established rat models were subdivided in to 1d, 3d, 7d, 21d,three time-phases. The changes of morphology in the injured cortex were observed by pathology; the real-time PCR was used to determine the expression level of P53mRNA in the injured cortex. Results: HE and pathological staining indicated that the nerve cells are arranged in order, the structure is normal, and the cell outline and the nucleus are clear at 21d in EA group. The expression of p53mRNA in 1d, 3d, 7d, 21d, four points in EA group were significantly increased than those in model group (P<0.05). Conclusion: EA plays a neuroprotective effect through promoting the expression of p53mRNA in hypoxic-ischemic brain tissuein rats.Downloads
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References
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[8]ZhengGQ. Methodological standards for experimental research on stroke using scalp acupuncture[J]. Acupuncture and Electro-the rapeuticRes, 2009, 34(1-2): 1-13. [9] Yu SG, Guo Y. experimental acupuncture[M]. Shanghai Science and Technology Press, 2014 :145. [10]Dey A, Verma CS, Lane DP. Modulation of p53 degradation as a therapeutic approach[J]. Cancer, 2008, 98(1):4-8． [11]Merino D, Malkin D. p53 and hereditary cancer［J]. SubcellBiochem, 2014, 85(1): 1-16. [12]The same as [2] .
[13]Liu TY,Guo HQ, Zhu MY, et al. Research progress of mutant p53 and its synthetic lethal gene[J].Hereditas(Beijing), 2015, 37(4): 321-326.
[4]MacPherson H, Hammerschlag R. Acupuncture and the emerging evidence base: contrived controversy and rational debate[J]. Acupunct Meridian Stud, 2012,5(4):141-147.
[5]Fang Z, Ning J, Xiong C, et al. Effects of electroacupuncture at head points on the function of cerebral motor areas in stroke patients: a PET study[J]. Evid Based Complement Alternat Med,2012:902413. [6]Xu T, Xu NG, Yang ZH, et al. Neuropotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B [J]. Chin JIntegrMed, 2015,12(1):1-10.
[7]Rice JE, Vannucci RC, Brierley JB, et al. The influence of immaturity on hypoxic-ischemic brain damage in the rat [J].Annals of neurology, 1981,9(2):131-141.
[8]ZhengGQ. Methodological standards for experimental research on stroke using scalp acupuncture[J]. Acupuncture and Electro-the rapeuticRes, 2009, 34(1-2): 1-13. [9] Yu SG, Guo Y. experimental acupuncture[M]. Shanghai Science and Technology Press, 2014 :145. [10]Dey A, Verma CS, Lane DP. Modulation of p53 degradation as a therapeutic approach[J]. Cancer, 2008, 98(1):4-8． [11]Merino D, Malkin D. p53 and hereditary cancer［J]. SubcellBiochem, 2014, 85(1): 1-16. [12]The same as [2] .
[13]Liu TY,Guo HQ, Zhu MY, et al. Research progress of mutant p53 and its synthetic lethal gene[J].Hereditas(Beijing), 2015, 37(4): 321-326.
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Published
2015-12-16
How to Cite
Zhang, H., Li, M., & Sun, Q. (2015). The Effect of Electroacupuncture on the Expression of p53 mRNA in Newborn Rats following Hypoxic-ischemic Encephalopathy. JOURNAL OF ADVANCES IN BIOLOGY, 8(3), 1629–1635. https://doi.org/10.24297/jab.v8i3.4494
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