Genetic distinction among cultivars of date palm using some molecular markers

Abstract

The experiment was carried out in the laboratories of biological techniques in the Department of Livestock, Agriculture College, Basrah University. The Randomly Amplified Polymerase Chain Reaction technique was used with four primers to find out the exactration DNA and the percentage of genetic divergence in six cultivars of date palm (Al_khasab, Allylwi,Bent Alsabaa, Dekal Ahmer, Alfersee and AlKhdrawee).Also, the study involved isolating DNA from the newly grown leaves of the study's date palm cultivars, and showing differences among the amplified pieces for each cultivar by deporting samples to the Electrophorses in the RAPD interactions .The results stated that there are considerable genetic differences among the cultivars of the current study. This was in terms of both the number of amplified bands and their molecular weight according to the primer used. The total number of the bands used was 64 for all primers. Moreover, the cultivars showed huge variations except ,Bent Alsabaa and Dekal Ahmer which stood at 75% and Dekal Ahmer and AlKhdrawee stood at 66.7%.The data was then collected and analyses, and the cultivars were classified into two separate groups. The cultivars Dekal Ahmer and Allylwi were in the same group where the former recorded less distance. Finally, the study concluded that the types are separated from each other reflected by phenotypic differences among them.