Antioxidant and Free Radical Scavenging Potential of Allium hookeri Thwaites Roots Extract Studied Using In Vitro Models

Abstract

Aim; To study antioxidant and free radical scavenging potential of Allium hookeri methanol roots extract (MREAH) using different in vitro models

Materials and Methods; Different in vitro models such as lipid peroxidation, metal chelation and reducing potential assays were used to determine antioxidant potential and 1, 1-diphenyl-2-picryl-hydrazil (DPPH,) superoxide (O₂^.-), hydrogen peroxide (H₂O₂), nitric oxide (NO^.) and hydroxyl radical (HO^.) radical scavenging assays for free radical scavenging abilities of MREAH.

Results; MREAH recorded concentration dependent effect on inhibition of lipid perodixation (IC₅₀= 90.15 ± 1.20μg/ml), effective metal chelation (IC₅₀=62.45 ± 1.27μg/ml) and higher reducing potential (OD_max=1.12 ± 0.20). MREAH could efficiently scavenged DPPH (IC₅₀ = 58.23±1.39μg/ml), O₂^.- (IC₅₀=140.30 ± 2.49μg/ml), H₂O₂ (IC₅₀=123.86 ± 3.00 μg/ml), NO ^.(IC₅₀ = 86.36 ± 2.01 μg/ml) and HO^.(IC₅₀ = 90.10 ± 1.80 μg/ml) radicals in dose dependent manner. The presence of polyphenol, flvanoids and ascorbic acids in the amounts 40.60 ± 1.10 mg/ml gallic acid equivalent-polyphenols and 28.44 ± 0.90 mg/ml quercetin equivalent-flavanoids and 24.20 ± 1.10 mg/ml ascorbic acid respectively may be attributed for these activities obtained per se in MREAH

Conclusion; These observations reveal antioxidant and free radical scavenging potentials of MREAH that support their therapeutic used with consequent health benefits.