Phytochemical screening and anticancer activity of leaf extracts of Physalis minima

Authors

  • H. N. Krishna Kumar Department of Studies in Biotechnology, Microbiology & Biochemistry Pooja Bhagavat Memorial Mahajana Education Centre, PG wing of SBRR Mahajana First Grade College, K.R.S. Road, Metagalli, Mysore-570 016, Karnataka
  • Jyoti Bala Chauh Department of Studies in Biotechnology, Microbiology & Biochemistry Pooja Bhagavat Memorial Mahajana Education Centre, PG wing of SBRR Mahajana First Grade College, K.R.S. Road, Metagalli, Mysore-570 016, Karnataka

DOI:

https://doi.org/10.24297/jns.v3i2.3971

Keywords:

Physalis minima, Anticancer activity, SRB assay, MTT assay, Phytochemicals

Abstract

Physalis minima a medicinally important plant of the family Solanaceae has been screened for its anticancer activity. The results of preliminary phytochemical screening of the leaf extract revealed the presence of flavonoids, alkaloids, tannins, saponins, steroids, cardiac glycosides, reducing sugars and terpenoids. Determination of total phenolic contents revealed that methanolic extract showed 78.3 mg/g of phenolic compounds. Evaluation of total flavonoid content showed 61.3 mg/g of flavonoid. It was found that the % viability of HeLa cell line & Hep2 cell line are 80% & 71.8% respectively. The percentage of growth inhibition of methanolic extract in SRB assay was found to be increase with increasing concentration against both HeLa and Hep2cell lines (68 and 58% respectively). The methanolic extract showed the strongest growth inhibitory effect on both HeLa and Hep2 cell lines (85 and 73% respectively) in MTT assay. Flavonoids have been isolated and purified from the methanolic leaf extract.

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Published

2016-10-31

How to Cite

Krishna Kumar, H. N., & Chauh, J. B. (2016). Phytochemical screening and anticancer activity of leaf extracts of Physalis minima. JOURNAL OF ADVANCES IN NATURAL SCIENCES, 3(2), 283–287. https://doi.org/10.24297/jns.v3i2.3971