Phytochemical screening and anticancer activity of leaf extracts of Physalis minima
DOI:
https://doi.org/10.24297/jns.v3i2.3971Keywords:
Physalis minima, Anticancer activity, SRB assay, MTT assay, PhytochemicalsAbstract
Physalis minima a medicinally important plant of the family Solanaceae has been screened for its anticancer activity. The results of preliminary phytochemical screening of the leaf extract revealed the presence of flavonoids, alkaloids, tannins, saponins, steroids, cardiac glycosides, reducing sugars and terpenoids. Determination of total phenolic contents revealed that methanolic extract showed 78.3 mg/g of phenolic compounds. Evaluation of total flavonoid content showed 61.3 mg/g of flavonoid. It was found that the % viability of HeLa cell line & Hep2 cell line are 80% & 71.8% respectively. The percentage of growth inhibition of methanolic extract in SRB assay was found to be increase with increasing concentration against both HeLa and Hep2cell lines (68 and 58% respectively). The methanolic extract showed the strongest growth inhibitory effect on both HeLa and Hep2 cell lines (85 and 73% respectively) in MTT assay. Flavonoids have been isolated and purified from the methanolic leaf extract.
Downloads
References
[2] Eva JM, Angel GL, Laura P, Ignacio A, Antonia C, Federico GA, BMC Cancer 2006, 6(1), 119.
[3] Hartwell JL, Lawrence, Massachusetts, 1982, 709 pp.
[4] The Wealth of India, National Institute of Science Communication and Information Resources, New Delhi, 2003, pp, 307-308.
[5] Kirtikar KR, Basu BD. Indian Medicinal Plants, 2008, 3, pp, 1766-1767.
[6] Parmar C, Kaushal MK, Kalyani Publishers, New Delhi, 1982, pp, 62-65.
[7] Sinha SC, Ray AB, J Indian Chem Soc, 1988, 65, 740-741.
[8] Vipin P, Ashok A, Indian J Trad Knowledge, 2010, 9(3), 519-521.
[9] Vardhana R, Sarup and Sons, 2008, p,270.
[10 ]Zakaria M, Mohamad MA, ITNM, 2010, pp, 175-185.
[11] Raju VS, Reddy CS, Rajarao KG, Acta Phytotaxonomica Sinica, 2007, 45(2), 239-245.
[12] Anisuzzaman M, Rahman AHMM, Harun-Or-Rashid M, Naderuzzaman ATM, Islam AKMR, J Appl Sci Res, 2007, 3(7), 519-530.
[13] Khandelwal KR, Practical Pharmacognosy, 2004, pp, 149-156.
[14] Singleton VL, Rossi JA, American Chemical Society Symposium series, 1965, 26, 47-70.
[15] Zhishen J, Mengcheng T, Jianming W, Food Chem, 1999, 64, pp. 555-559.
[16] Phillips HJ, Terryberry JE, Cell Research, 1957, 13, 341-347.
[17] Masters RW, Animal cell culture, 2000, p, 1 - 3.
[18] Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Journal National Cancer Institute, 1990, 82(13), 1107-1112.
[19] Masters RW, Animal cell culture, 2000, p, 202-203.
[20] Mosmann T, Journal of Immunology Methods, 1983, 65, 55-63.
[21] Masters RW, Animal cell culture, 2000, p, 207.
[22] Jainu M, Devi CS, Journal of Ethnopharmacology, 2006, 104, 156-63.
[23] Shishodia S, Aggarwal BB, Oncogene , 2006, 25, 1463-73.
[24] Heinrich M, Bremner P, Curr Drug Targets, 2006, 7, 239.
[25] Larson RA, Phytochem, 1988, 27, 969- 978.
[26] Tanaka M, Kuei CW, Nagashima Y, Taguchi T, Nippon Suisan Gakkaishi, 1998, 54, 1409 -1414.
[27] Leong OK, Tengku Muhammad TS, Sulaiman SF, Evid-Based Complent Altern Med eCAM, 2009, p, 1-10.
[28] Leong OK, Tengku Muhammad TS, Integr Cancer Ther, 2010, 9(1), 73-83.
[29] Leong OK, Tengku Muhammad TS, Sulaiman SF, J Ethnopharmacol, 2010, 28(1-2), 92-99.
[30] Daya L Chothani, Vaghasiya HU, Indian Journal of Natural Products and Resources, 2012, 3(4), 477-482.
Downloads
Published
How to Cite
Issue
Section
License
All articles published in Journal of Advances in Linguistics are licensed under a Creative Commons Attribution 4.0 International License.
