Antioxidant and Free Radical Scavenging Potential of Allium hookeri Thwaites Roots Extract Studied Using In Vitro Models
Aim; To study antioxidant and free radical scavenging potential of Allium hookeri methanol roots extract (MREAH) using different in vitro models
Materials and Methods; Different in vitro models such as lipid peroxidation, metal chelation and reducing potential assays were used to determine antioxidant potential and 1, 1-diphenyl-2-picryl-hydrazil (DPPH,) superoxide (O2.-), hydrogen peroxide (H2O2), nitric oxide (NO.) and hydroxyl radical (HO.) radical scavenging assays for free radical scavenging abilities of MREAH.
Results; MREAH recorded concentration dependent effect on inhibition of lipid perodixation (IC50= 90.15 ± 1.20Î¼g/ml), effective metal chelation (IC50=62.45 ± 1.27Î¼g/ml) and higher reducing potential (ODmax=1.12 ± 0.20). MREAH could efficiently scavenged DPPH (IC50 = 58.23±1.39Î¼g/ml), O2.- (IC50=140.30 ± 2.49Î¼g/ml), H2O2 (IC50=123.86 ± 3.00 Î¼g/ml), NO . (IC50 = 86.36 ± 2.01 Î¼g/ml) and HO. (IC50 = 90.10 ± 1.80 Î¼g/ml) radicals in dose dependent manner. The presence of polyphenol, flvanoids and ascorbic acids in the amounts 40.60 ± 1.10 mg/ml gallic acid equivalent-polyphenols and 28.44 ± 0.90 mg/ml quercetin equivalent-flavanoids and 24.20 ± 1.10 mg/ml ascorbic acid respectively may be attributed for these activities obtained per se in MREAH
Conclusion; These observations reveal antioxidant and free radical scavenging potentials of MREAH that support their therapeutic used with consequent health benefits.
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