TY - JOUR AU - Aravanopoulos, Filippos A. (Phil) AU - Tchatchoua, Dorothy T. AU - Barbas, Evangelos PY - 2014/06/21 Y2 - 2024/03/28 TI - Micropropagation of Elite Genotypes of Castanea Sativa (MILL.) JF - JOURNAL OF ADVANCES IN BIOTECHNOLOGY JA - JBT VL - 3 IS - 2 SE - Articles DO - 10.24297/jbt.v3i2.1679 UR - https://rajpub.com/index.php/jbt/article/view/1679 SP - 200-209 AB - <p>Sprouted buds of eight mature plus-trees of C<em>astanea sativa</em> were collected from two natural populations (Mt. Paiko and Mt. Hortiatis, Greece). Explants were cultured on Murashige and Skoog (MS) medium (three concentrations of macro and micro-elements) combined with three concentrations of BAP (6-Benzylaminopurine). Results were evaluated based on three multiplication traits: (a) number of shoots produced, (b) length of the longest shoot segments and (c) percentage of responsive explants. A high genotype-dependant variation for the number of shoots produced per explant was detected. MS medium at full salt strength and 0.2 mg l<sup>-</sup><sup>1</sup> BAP provided the best results based on the multiplication traits measured. Elite mature tree P-2 proved to be highly favourable for multiplication. Rooting was attempted on ½ MS medium at five different NAA concentrations. Successful rooting (56%) was obtained by using 0.50 mg l<sup>-</sup><sup>1</sup> NAA in vermiculite medium after eight weeks. These results indicate that the P-2 genotype can be micropropagated from the protocol developed for ornamental purposes, clonal testing and tree improvement applications.</p> ER -