TY - JOUR AU - zaki, sahar PY - 2016/03/16 Y2 - 2024/03/28 TI - Biogenic synthesis of Iron oxide nanoparticles via optimization of nitrate reductase Enzyme using statistical experimental design JF - JOURNAL OF ADVANCES IN BIOTECHNOLOGY JA - JBT VL - 5 IS - 2 SE - Articles DO - 10.24297/jbt.v5i2.1575 UR - https://rajpub.com/index.php/jbt/article/view/1575 SP - 667-684 AB - <p>Statistically designed experiments were proceeded to evaluate nutritional and environmental parameters that affect nitrate reductase enzyme (NR) activity in <a title="Go to alignment for Achromobacter xylosoxidans strain NSBx.33(HE2) 16S ribosomal RNA gene, partial sequence" href="https://blast.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_324035673"><em>Achromobacter sp.</em><strong> KT735046</strong></a>. Plackett-Burman design was performed for screening and identifying efficiently the significance of 15 culture conditions influencing NR activity. FeCl<sub>3&nbsp;</sub>·6H<sub>2</sub>O, Na<sub>2</sub>MoO<sub>4&nbsp;</sub>·2H<sub>2</sub>O and CuSO<sub>4</sub>.5H<sub>2</sub>O were the most significant variables positively influencing, whereas pH was the most significant negative contributors. The optimal levels of significant factors were further predicted from five level factorial designs, Central Composite Design (CCD). The optimum parameter values were CuSO<sub>4</sub>.5H<sub>2</sub>O (60.375) mg/l, FeCl<sub>3</sub>·6H<sub>2</sub>O (240 mg /L), pH (6.135) and Na<sub>2</sub>MoO4·2H<sub>2</sub>O (150 mg /L). The biogenic Iron oxide nanoparticles "IONPs" were characterized; the dark brown IONPs exhibit maximum absorption from 400 to 464 nm, XRD reveals crystallite rhombohedral hematite, EDX confirms presence of 62% of iron, TEM illustrates formation of tiny IONPs, 1.4 and 2.8 nm in size, ξ potential and PDI recorded -42.9 mV and 0.268 respectively exhibiting high stability and monodispersed with no agglomeration by the help of biomolecules. This study conclude that <a title="Go to alignment for Achromobacter xylosoxidans strain NSBx.33(HE2) 16S ribosomal RNA gene, partial sequence" href="https://blast.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_324035673"><em>Achromobacter</em> sp.</a> KT735046 consider being biofactory for synthesis nanoparticles by the help of NR enzyme and it may be the first time to optimize <a title="Go to alignment for Achromobacter xylosoxidans strain NSBx.33(HE2) 16S ribosomal RNA gene, partial sequence" href="https://blast.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_324035673"><em>Achromobacter</em> sp.</a> KT735046 for IONPs biosynthesis using statistical designs.&nbsp;</p> ER -