@article{Sarropoulou_Maloupa_2019, title={Micropropagation and ex situ conservation of Silene fabaria (L.) Sm. in Sibth. & Sm. subsp. domokina Greuter (Caryophyllaceae); an important endemic plant in Greece with medicinal and ornamental value}, volume={8}, url={https://rajpub.com/index.php/jbt/article/view/8062}, DOI={10.24297/jbt.v8i0.8062}, abstractNote={<p><em>Silene</em> species (Caryophyllaceae) are sources of important secondary metabolites with extensive use in traditional medicine and potential applications as ornamentals. The present study was conducted to assess the regeneration potential of <em>Silene fabaria</em> subsp. <em>domokina </em>to produce massive clonal <em>in vitro</em> plants. Two experiments were conducted. The basal culture medium used was the MS. In the first experiment, the effect of 3 cytokinins; BA, KIN and 2-ip applied alone and in combination with 3 auxins; IBA, NAA and IAA was studied. In the second experiment, the effect of 3 auxins; IBA, NAA and IAA, each applied in 3 different concentrations (0.1, 0.25 and 0.5 mg/l) was studied. Shoot proliferation 100%, highest shoot proliferation rate (4.83) and shoot number (3.67) were achieved with 0.25 mg/l BA and 0.1 mg/l IAA (5 weeks). IAA at 0.5 mg/l was the most effective in stimulating shoot elongation (80.63 mm). Rooting 100% was obtained with 0.1 mg/l IBA yielding 7.3 roots 22.91 mm long (4 weeks). <em>In vitro</em> plants were successfully acclimatized with 92.31% survival rate. This study is the first micropropagation report of <em>S. fabaria</em> subsp. <em>domokina</em> that could be exploited for rapid, large-scale production and future germplasm maintenance of this valuable prioritized species-subspecies.</p>}, journal={JOURNAL OF ADVANCES IN BIOTECHNOLOGY}, author={Sarropoulou, Virginia and Maloupa, Eleni}, year={2019}, month={Feb.}, pages={1044–1057} }