@article{Antakli_Nejem_Alraii_2021, title={Determination of Glibenclamide By Analytical Spectrophotometry}, volume={18}, url={https://rajpub.com/index.php/jac/article/view/8963}, DOI={10.24297/jac.v18i.8963}, abstractNote={<p>Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λ<sub>max</sub> = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R<sup>2</sup> = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively.  First derivative spectrophotometric (1DS) method was applied for the determination of (GB) in the presence (MET). (GB) was determined at 317 nm (1D317). Linearity ranges were (4 – 240) μg/mL for (GB). Regression analysis showed a good correlation coefficients R<sup>2</sup> = 0.999914. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.60 μg/mL and 1.83 μg/mL for (GB).</p> <p>The proposed zero spectrophotometry method was applied to analysis individual (GB), and the derivative (<sup>1</sup>D<sub>317</sub>) method was applied to analysis (GB) individually or combined with (MET) in Syrian trademark drugs.</p> <p>The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.</p>}, journal={JOURNAL OF ADVANCES IN CHEMISTRY}, author={Antakli, Saad and Nejem, Leon and Alraii, Monzer}, year={2021}, month={Apr.}, pages={40–48} }