TY - JOUR AU - Wang, Junwei AU - Zhao, Ge AU - Gao, Yubin AU - Xu, Hefei AU - Mohamed, Lounis AU - Zhao, Jianmei AU - Gai, Wenyan AU - Zou, Ming AU - Cui, Zhigang AU - Yan, Shigan AU - Wang, Juan AU - Qu, Zhina PY - 2019/09/20 Y2 - 2024/03/28 TI - Virulence and Antimicrobial Characteristics of Escherichia Coli Isolated from Diseased Chickens in China and Algeria JF - JOURNAL OF ADVANCES IN AGRICULTURE JA - JAA VL - 10 IS - SE - Articles DO - 10.24297/jaa.v10i0.8425 UR - https://rajpub.com/index.php/jaa/article/view/8425 SP - 1821-1833 AB - <p>To reveal and compare the prevalence of pathotypes and virulence genes, as well as antimicrobial resistance and genotyping of poultry<em> E. coli</em> isolates from China and Algeria. Pathotype and seven virulence genes were tested by PCR, susceptibility to antimicrobials was evaluated using broth microdilution method, and genotyping was analyzed by PFGE. Six isolates were identified as pathogenic <em>E. coli</em>. Virulence gene testing showed that the frequency of<em> ompT, iss, fimC, iroN, hlyF</em> and<em> iutA</em> was high in the isolates from Shandong, Shanxi, Jiangsu and Xinjiang province, while<em> stx2</em> was detected in only two isolates from Shandong, and <em>stx2, iss, fimC, hlyF </em>and <em>iutA</em> could not be detected in Tibet isolates. Importantly, nearly all isolates from Algeria carried seven virulence genes. Drug resistance testing of 141 strains showed that 98.2% (109/111) of the isolates from China and all isolates (30/30) from Algeria resist to more than three classes of antimicrobials. The PFGE genotyping of 157 isolates yielded 134 types, demonstrated a high level of genetic diversity among these isolates. Thus, the poultry<em> E. coli</em> from both China and Algeria exhibited either high frequencies of antimicrobial resistance or high rates of virulence genes carrying.</p> ER -