TY - JOUR AU - Mohamed, Nema Abdelhameed AU - Ali, Awatef Mohamed AU - Ghareeb, Doaa Ahmed AU - Mohamed, Adham Rashed AU - Elmokhtar, Yasmin Mohamed PY - 2018/04/30 Y2 - 2024/03/28 TI - The Curative Effect of Berberine Nanoparticles and Cisplatin Combination Therapies Against Hepatocarcinogenesis-Induced By N-Nitroso-Diethylamine In Male Rat JF - JOURNAL OF ADVANCES IN BIOLOGY JA - JAB VL - 11 IS - SE - Articles DO - 10.24297/jab.v11i1.7207 UR - https://rajpub.com/index.php/jab/article/view/7207 SP - 2180-2200 AB - <p>This study aimed to investigate whether berberine nanoparticles (BBR-NPs) and/or cisplatin supplementation could prevent hepatocarcinogenesis-induced by N-nitroso-diethylamine (DENA) in male rats. Male <em>Wistar</em> albino rats were divided into five groups; Group 1: Control; Group 2: DENA-CCl<sub>4</sub>; Group 3: DENA-CCl<sub>4</sub>+Cisplatin; Group 4: DENA-CCl<sub>4</sub>+BBR-NPs; Group 5: DENA-CCl<sub>4</sub>+Cisplatin+BBR-NPs. DENA-CCl<sub>4</sub> significantly increase AST, ALT, ALP, LDH, GGT, AFP activities and total bilirubin, while, 5<sup>, </sup>NT,&nbsp; total protein and albumin decreased. DENA-CCl<sub>4</sub> treatment caused increment in MDA levels and reduction in SOD, CAT, GPx and GSH in liver tissues. Moreover, DENA-CCl<sub>4</sub> increase the gene expression of ADAM17 and TNF-a however, &nbsp;P53 was declined. In addition, DENA-CCl<sub>4</sub> caused severe histopathological lesions in the liver tissue. Interestingly, administration of berberine nanoparticles alone or in combination with cisplatin improves the hepatocarcinogenesis induced by DENA-CCl<sub>4</sub> on the physiological, biochemical, molecular and histological levels by decreasing oxidative stress and preserving gene expression of ADAM17, TNF-I and P53. The present findings suggest that BBR-NPs with cisplatin might offer a promising strategy for the prevention of liver cancer.</p> ER -